Organotypic Endothelial Cell Surface Molecules Mediate Organ Preference of Metastasis

1992 ◽  
pp. 439-454 ◽  
Author(s):  
Bendicht U. Pauli ◽  
Robert C. Johnson ◽  
Marwan E. El-Sabban
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Cell Surface Molecules ◽  
Endothelial Cell Surface ◽  
Surface Molecules ◽  
Organ Preference

Sickle reticulocytes adhere to VCAM-1

Blood ◽  
1995 ◽  
Vol 85 (1) ◽  
pp. 268-274 ◽  
Author(s):  
BE Gee ◽  
OS Platt
Keyword(s):  
Endothelial Cell ◽  
Transferrin Receptor ◽  
Cell Adhesion Molecules ◽  
Low Density ◽  
Cell Surface Molecules ◽  
Cos Cells ◽  
Endothelial Cell Surface ◽  
Surface Molecules ◽  
Endothelial Cell Adhesion Molecules ◽  
Endothelial Cell Adhesion

Adherence of sickle (SS) erythrocytes to endothelial cells represents interactions between red blood cell (RBC) and endothelial cell surface molecules. To enhance our understanding of the ligands involved, we transfected COS cells with the cDNAs of two endothelial cell adhesion molecules, VCAM-1 and E-selectin, and measured the binding of normal and sickle RBCs after static incubation. The percentage of COS cells with rosettes (five or more adherent RBCs) was determined. Normal RBCs did not adhere to VCAM-1-transfected COS cells. Unfractionated SS RBCs formed rosettes on the VCAM-1-transfected COS cells (mean +/- SD, 5.85% +/- 4.98%). Low-density SS RBCs were more adherent than high-density SS RBCs (P < .005). The adherent SS RBCs were a young reticulocyte population, staining positively for transferrin receptor. Another measure of reticulocyte age, RNA content, also correlated with adherence. SS RBC binding was specific--inhibitable by antibodies to either VCAM-1 or the alpha 4 integrin chain of VLA-4. In contrast, there was no significant adherence of normal or SS RBCs to E-selectin- transfected COS cells. These results suggest that young reticulocytes bind to endothelial cell VCAM-1 via VLA-4 integrin.

scholarly journals Cell surface molecules that bind fibronectin's matrix assembly domain

1991 ◽  
Vol 266 (15) ◽  
pp. 9697-9702 ◽  
Author(s):  
A.H. Limper ◽  
B.J. Quade ◽  
R.M. LaChance ◽  
T.M. Birkenmeier ◽  
T.S. Rangwala ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Surface Molecules ◽  
Matrix Assembly ◽  
Surface Molecules

Cell surface molecules (NCAM and LI) in intrastriatal transplants of embryonic mesencephalon in rats

Neuroscience ◽  
1996 ◽  
Vol 73 (1) ◽  
pp. 161-169 ◽  
Author(s):  
G. Gopinath ◽  
V. Sable ◽  
K. Sailaja ◽  
P.N. Tandon
Keyword(s):  
Cell Surface ◽  
Cell Surface Molecules ◽  
Surface Molecules
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