Phage Display Breast Carcinoma cDNA Libraries: Isolation of Clones Which Specifically Bind to Membrane Glycoproteins, Mucins, and Endothelial Cell Surface

2000 ◽  
Author(s):  
Fumiichiro Yamamoto
Keyword(s):  
Endothelial Cell ◽  
Breast Carcinoma ◽  
Cell Surface ◽  
Phage Display ◽  
Cdna Libraries ◽  
Membrane Glycoproteins ◽  
Endothelial Cell Surface

scholarly journals Surface charge distribution on the endothelial cell of liver sinusoids.

1984 ◽  
Vol 99 (2) ◽  
pp. 639-647 ◽  
Author(s):  
L Ghitescu ◽  
A Fixman
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Liver Sinusoid ◽  
Electron Microscopic ◽  
Coated Pits ◽  
Endothelial Cell Surface ◽  
Liver Sinusoids ◽  
Surface Charge Distribution ◽  
Cationic Residues

The topography of the charged residues on the endothelial cell surface of liver sinusoid capillaries was investigated by using electron microscopic tracers of different size and charge. The tracers used were native ferritin (pl 4.2-4.7) and its cationized (pl 8.4) and anionized (pl 3.7) derivatives, BSA coupled to colloidal gold (pl of the complex 5.1), hemeundecapeptide (pl 4.85), and alcian blue (pl greater than 10). The tracers were either injected in vivo or perfused in situ through the portal vein of the mouse liver. In some experiments, two tracers of opposite charge were sequentially perfused with extensive washing in between. The liver was processed for electron microscopy and the binding pattern of the injected markers was recorded. The electrostatic nature of the tracer binding was assessed by perfusion with high ionic strength solutions, by aldehyde quenching of the plasma membrane basic residues, and by substituting the cell surface acidic moieties with positively charged groups. Results indicate that the endothelial cells of the liver sinusoids expose on their surface both cationic and anionic residues. The density distribution of these charged groups on the cell surface is different. While the negative charge is randomly and patchily scattered all over the membrane, the cationic residues seem to be accumulated in coated pits. The charged groups co-exist in the same coated pit and bind the opposite charged macromolecule. It appears that the fixed positive and negative charges of the coated pit glycocalyx are mainly segregated in space. The layer of basic residues is located at 20-30-nm distance of the membrane, while most of the negative charges lie close to the external leaflet of the plasmalemma.

The ultrastructural basis of endothelial cell surface functions

Biorheology ◽  
1984 ◽  
Vol 21 (1-2) ◽  
pp. 155-170 ◽  
Author(s):  
Una S. Ryan ◽  
James W. Ryan
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Endothelial Cell Surface

scholarly journals Leukocyte adhesion is governed by endolysosomal two pore channel 2 (TPC2)

2021 ◽  
Author(s):  
Jonas Goretzko ◽  
Nicole Heitzig ◽  
Katharina Thomas ◽  
Einar Kleinhans Krogsaeter ◽  
Johannes Nass ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Leukocyte Adhesion ◽  
Leukocyte Recruitment ◽  
Pore Channel ◽  
Initial Contact ◽  
Surface Receptors ◽  
Endothelial Cell Surface ◽  
Late Endosomes ◽  
Endothelial Functions

In response to pro-inflammatory challenges including pathogenic attack and tissue damage, the endothelial cell surface is rearranged to present leukocyte-engaging cell surface receptors. The initial contact needed for leukocyte tethering and rolling is mediated via adhesion demand-driven exocytosis of Weibel-Palade bodies (WPB) that contain the leukocyte receptor P-selectin together with the stabilizing co-factor CD63. We found that diminished expression of the endolysosomal non-selective cation channel TPC2 or inhibition of TPC2-mediated Ca2+-release via trans-Ned 19 led to reduced endolysosomal Ca2+ efflux, and blocked transfer of CD63 from late endosomes/lysosomes (LEL) to WPB, and a concomitant loss of P-selectin on the endothelial cell surface. Accordingly, P-selectin-mediated leukocyte recruitment to trans-Ned 19-treated HUVEC under flow was significantly reduced without disturbing VWF exocytosis. Our findings establish the endolysosome-related TPC2 Ca2+ channel as a key element in the maintenance of proper endothelial functions and a potential pharmacological target in the control of inflammatory leukocyte recruitment.

scholarly journals An Inhibitor of the F1 Subunit of ATP Synthase (IF1) Modulates the Activity of Angiostatin on the Endothelial Cell Surface

2004 ◽  
Vol 27 (6) ◽  
pp. S7 ◽  
Author(s):  
Nick Burwick ◽  
Miriam Wahl ◽  
Daniel Kenan ◽  
Salvatore Pizzo
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Atp Synthase ◽  
Endothelial Cell Surface

Plasma and endothelial cell-surface adhesion molecules and cardiopulmonary bypass

2000 ◽  
Vol 9 (3) ◽  
pp. A185
Author(s):  
Michael P. Vallely ◽  
Paul G. Bannon ◽  
Clifford F. Hughes ◽  
Leonard Kritharides
Keyword(s):  
Cardiopulmonary Bypass ◽  
Endothelial Cell ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Surface Adhesion ◽  
Endothelial Cell Surface
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