Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes

2015 ◽  
pp. 43-46 ◽  
Author(s):  
Handan Yavuz ◽  
Nilay Bereli ◽  
Fatma Yılmaz ◽  
Canan Armutcu ◽  
Adil Denizli
Keyword(s):  
Human Plasma ◽  
Antibody Purification ◽  
Affinity Membranes

Antibody purification from human plasma by metal-chelated affinity membranes

2011 ◽  
Vol 123 (6) ◽  
pp. 3476-3484 ◽  
Author(s):  
Handan Yavuz ◽  
Nilay Bereli ◽  
Canan Armutçu ◽  
Fatma Yılmaz ◽  
Adil Denizli
Keyword(s):  
Human Plasma ◽  
Antibody Purification ◽  
Affinity Membranes

Metal-Chelating Nanopolymers for Antibody Purification from Human Plasma

2012 ◽  
Vol 168 (6) ◽  
pp. 1528-1539 ◽  
Author(s):  
Deniz Aktaş Uygun ◽  
R. Hilal Şenay ◽  
Ceren Türkcan ◽  
Sinan Akgöl ◽  
Adil Denizli
Keyword(s):  
Human Plasma ◽  
Antibody Purification ◽  
Metal Chelating

Performance of Protein-A-Based Affinity Membranes for Antibody Purification

2011 ◽  
Vol 22 (17) ◽  
pp. 2325-2341 ◽  
Author(s):  
Lokman Uzun ◽  
Deniz Türkmen ◽  
Veyis Karakoç ◽  
Handan Yavuz ◽  
Adil Denizli
Keyword(s):  
Protein A ◽  
Antibody Purification ◽  
Affinity Membranes

Performances of an Artificial Reagent for the One-stage Factor IX Assay

1975 ◽  
Vol 33 (03) ◽  
pp. 547-552 ◽  
Author(s):  
L Meunier ◽  
J. P Allain ◽  
D Frommel
Keyword(s):  
Human Plasma ◽  
Factor Ix ◽  
Severe Haemophilia ◽  
Normal Human Plasma ◽  
Haemophilia B ◽  
One Stage ◽  
Normal Human ◽  
The One

SummaryA mixture of adsorbed normal human plasma and chicken plasma was prepared as reagent for factor IX measurement using a one-stage method. The substrate was found to be specific for factor IX. Its performances tested on samples displaying factor IX activity ranging from <l%–2,500% compared favorably with those obtained when using the plasma of severe haemophilia B patients as substrate.

Plasma Components which Interfere with Ristocetin-induced Platelet Aggregation

1975 ◽  
Vol 33 (03) ◽  
pp. 540-546 ◽  
Author(s):  
Robert F Baugh ◽  
James E Brown ◽  
Cecil Hougie
Keyword(s):  
Platelet Aggregation ◽  
Human Plasma ◽  
Plasma Proteins ◽  
Binding Protein ◽  
Plasma Heating ◽  
Protein S ◽  
Fold Increase ◽  
Normal Human Plasma ◽  
Preliminary Examination ◽  
Normal Human

SummaryNormal human plasma contains a component or components which interfere with ristocetin-induced platelet aggregation. Preliminary examination suggests a protein (or proteins) which binds ristocetin and competes more effectively for ristocetin than do the proteins involved in ristocetin-induced platelet aggregation. The presence of this protein in normal human plasma also prevents ristocetin-induced precipitation of plasma proteins at levels of ristocetin necessary to produce platelet aggregation (0.5–2.0 mg/ml). Serum contains an apparent two-fold increase of this component when compared with plasma. Heating serum at 56° for one hour results in an additional 2 to 4 fold increase. The presence of a ristocetin-binding protein in normal human plasma requires that this protein be saturated with ristocetin before ristocetin-induced platelet aggregation will occur. Variations in the ristocetin-binding protein(s) will cause apparent discrepancies in ristocetin-induced platelet aggregation in normal human plasmas.

Sign in / Sign up

Export Citation Format

Share Document