Shoot Tip Meristem Cryopreservation of Hypericum Species

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

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Molecular and Biotechnological Advancements in Hypericum Species

10.3389/978-2-88945-117-3 ◽

2017 ◽

Keyword(s):

Hypericum Species

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Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.3.18 ◽

2020 ◽

Vol 80 (03) ◽

Author(s):

Rupesh S. Badere ◽

Pallavi K. Rinkey

Keyword(s):

Plant Growth Regulator ◽

Gamma Ray ◽

Multiple Shoot ◽

Shoot Tip ◽

Ms Medium ◽

Shoot Induction ◽

Ethyl Methane Sulphonate ◽

Multiple Shoot Induction ◽

Methane Sulphonate ◽

Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

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HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species

Revista de Chimie ◽

10.37358/rc.18.2.6095 ◽

2018 ◽

Vol 69 (2) ◽

pp. 305-309 ◽

Cited By ~ 2

Author(s):

Daniela Gitea ◽

Simona Vicas ◽

Manuel Alexandru Gitea ◽

Sebastian Nemeth ◽

Delia Mirela Tit ◽

...

Keyword(s):

Antioxidant Capacity ◽

Methanol Extract ◽

Antioxidant Properties ◽

Polyphenolic Compounds ◽

The Other ◽

The North ◽

Western Area ◽

North Western ◽

Hypericum Species ◽

Biological Actions

Our study compares the content in polyphenolic compounds and hypericin, in four species of Hypericum - H. perforatum L., H. maculatum Cr., H. hirsutum L., H. tetrapterum Fr. (syn. Hypericumacutum Mnch.) harvested from spontaneous flora in the north-western area of Transylvania, Romania. These species represent an important source of such compounds with different biological actions. After making the extracts, they were subjected to HPLC-SM analysis. The presence of rutoside in the largest amount (462.82 mg %) in the H. perforatum extract was observed, this containing most of the flavonoid heterosides. For the species H. maculatum, the presence in a much higher amount of the hyperoside (976.36 mg %) is characteristic compared to the other species. Quercetol is the best represented of the flavonoid aglycons, its concentration being the highest in H. hirsutum (659.66 mg %). The hypericin content ranges from 0.2171 g % in the H. tetrapterum extract, to 0.0314 g % in the methanol extract of H. maculatum.The highest antioxidant properties measured by FRAP method were recorded in the case of H. perforatum and H. maculatum.

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Sterilization And Establishment Of Pineapple Shoot Tip

10.37376/1570-000-016-002 ◽

2017 ◽

pp. 1

Author(s):

Abdelhamid M. Hamad

Keyword(s):

Shoot Tip

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Herbicide Tolerance Of Transgenic `Stevens' Cranberry Plants Depends on the Test Environment

HortScience ◽

10.21273/hortsci.33.3.517a ◽

1998 ◽

Vol 33 (3) ◽

pp. 517a-517

Author(s):

Eric L. Zeldin ◽

Rodney A. Serres ◽

Brent H. McCown

Keyword(s):

Growth Form ◽

Herbicide Tolerance ◽

Genetically Engineered ◽

Shoot Tip ◽

Test Environment ◽

Growth Environment ◽

Commercial Formulation ◽

Tolerance Level ◽

Delayed Growth ◽

Significant Injury

`Stevens' cranberry was genetically engineered to confer tolerance to the broad spectrum herbicide glufosinate. Initially, herbicide tolerance was verified by spraying greenhouse plants with the commercial formulation Liberty. Although one transformant showed significant tolerance, the tolerance level was below that required to kill goldenrod, a common weed of cranberry beds. This transformant was propagated and the plants established outdoors in a coldframe, yielding a growth form more typical of field-grown plants than that of greenhouse-grown plants. These plants, as well as untransformed cranberry and goldenrod plants, were sprayed with various levels of the herbicide. The transformed plants were not killed at glufosinate concentrations up to 1000 ppm, although delayed growth did occur. Some runner tip injury was observed at 500 ppm as well as widespread shoot tip death at higher levels. The above-ground parts of goldenrod plants were killed at 400 ppm with significant injury at 200 ppm. Untransformed cranberry plants were killed at 300 ppm and had extensive tip death even at 100 ppm. Transformed cranberry plants with confirmed “field” tolerance were re-established in the greenhouse and new vegetative growth was forced. When these plants were sprayed with glufosinate, significant shoot tip injury was observed at levels as low as 100 ppm. The degree of herbicide tolerance of transformed cranberry appears to be modulated by the growth environment, which may affect the expression of the inserted genes or the physiological sensitivity of the impacted tissues.

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