Membrane Protein Production in Lactococcus lactis for Functional Studies

2016 ◽  
pp. 79-101 ◽  
Author(s):  
Daphne Seigneurin-Berny ◽  
Martin S. King ◽  
Emiline Sautron ◽  
Lucas Moyet ◽  
Patrice Catty ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lactococcus Lactis ◽  
Protein Production ◽  
Functional Studies

scholarly journals The Response of Lactococcus lactis to Membrane Protein Production

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e24060 ◽  
Author(s):  
Ravi K. R. Marreddy ◽  
Joao P. C. Pinto ◽  
Justina C. Wolters ◽  
Eric R. Geertsma ◽  
Fabrizia Fusetti ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lactococcus Lactis ◽  
Protein Production

scholarly journals Membrane Protein Stabilization Strategies for Structural and Functional Studies

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 155
Author(s):  
Ekaitz Errasti-Murugarren ◽  
Paola Bartoccioni ◽  
Manuel Palacín
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Lipid Membrane ◽  
Protein Stabilization ◽  
New Drugs ◽  
Cell Physiology ◽  
Protein Preparation ◽  
Functional Studies ◽  
Membrane Protein Stability ◽  
Druggable Targets

Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.

scholarly journals Membrane protein production inEscherichia colicell-free lysates

FEBS Letters ◽  
2015 ◽  
Vol 589 (15) ◽  
pp. 1713-1722 ◽  
Author(s):  
Erik Henrich ◽  
Christopher Hein ◽  
Volker Dötsch ◽  
Frank Bernhard
Keyword(s):  
Membrane Protein ◽  
Protein Production

Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production

2016 ◽  
Vol 6 (2) ◽  
pp. 284-300 ◽  
Author(s):  
Dimitra Gialama ◽  
Kalliopi Kostelidou ◽  
Myrsini Michou ◽  
Dafni Chrysanthi Delivoria ◽  
Fragiskos N. Kolisis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production ◽  
High Level

Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression

2014 ◽  
Vol 31 (6) ◽  
pp. 538-552 ◽  
Author(s):  
Thomas Vogl ◽  
Gerhard G. Thallinger ◽  
Guenther Zellnig ◽  
David Drew ◽  
James M. Cregg ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Membrane Protein ◽  
Protein Production ◽  
Transcriptional Response ◽  
Protein Overexpression
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