scholarly journals Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay

2016 ◽  
pp. 193-202 ◽  
Author(s):  
Ran Yan ◽  
Dawei Cai ◽  
Yuanjie Liu ◽  
Haitao Guo
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cultured Cells ◽  
Virus Particles ◽  
B Virus

scholarly journals ssAAVs containing cassettes encoding SaCas9 and guides targeting hepatitis B virus inactivate replication of the virus in cultured cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Tristan Scott ◽  
Buhle Moyo ◽  
Samantha Nicholson ◽  
Mohube Betty Maepa ◽  
Koichi Watashi ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cultured Cells ◽  
B Virus

scholarly journals Engineered External Guide Sequences Are Highly Effective in Inhibiting Gene Expression and Replication of Hepatitis B Virus in Cultured Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65268 ◽  
Author(s):  
Zhigang Zhang ◽  
Gia-Phong Vu ◽  
Hao Gong ◽  
Chuan Xia ◽  
Yuan-Chuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cultured Cells ◽  
B Virus ◽  
Highly Effective

scholarly journals Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

2013 ◽  
Vol 21 (10) ◽  
pp. 1889-1897 ◽  
Author(s):  
Kristie Bloom ◽  
Abdullah Ely ◽  
Claudio Mussolino ◽  
Toni Cathomen ◽  
Patrick Arbuthnot
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Virus Replication ◽  
Cultured Cells ◽  
Transcription Activator ◽  
B Virus

scholarly journals MicroRNA 130a Regulates both Hepatitis C Virus and Hepatitis B Virus Replication through a Central Metabolic Pathway

2018 ◽  
Vol 92 (7) ◽  
Author(s):  
Xiaoqiong Duan ◽  
Shilin Li ◽  
Jacinta A. Holmes ◽  
Zeng Tu ◽  
Yujia Li ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Target Genes ◽  
Viral Infections ◽  
Cultured Cells ◽  
Guide Rna ◽  
Hbv Replication ◽  
B Virus

ABSTRACTHepatitis C virus (HCV) infection has been shown to regulate microRNA 130a (miR-130a) in patient biopsy specimens and in cultured cells. We sought to identify miR-130a target genes and to explore the mechanisms by which miR-130a regulates HCV and hepatitis B virus (HBV) replication. We used bioinformatics software, including miRanda, TargetScan, PITA, and RNAhybrid, to predict potential miR-130a target genes. miR-130a and its target genes were overexpressed or were knocked down by use of small interfering RNA (siRNA) or clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 guide RNA (gRNA). Selected gene mRNAs and their proteins, together with HCV replication in OR6 cells, HCV JFH1-infected Huh7.5.1 cells, and HCV JFH1-infected primary human hepatocytes (PHHs) and HBV replication in HepAD38 cells, HBV-infected NTCP-Huh7.5.1 cells, and HBV-infected PHHs, were measured by quantitative reverse transcription-PCR (qRT-PCR) and Western blotting, respectively. We selected 116 predicted target genes whose expression was related to viral pathogenesis or immunity for qPCR validation. Of these, the gene encoding pyruvate kinase in liver and red blood cell (PKLR) was confirmed to be regulated by miR-130a overexpression. miR-130a overexpression (via a mimic) knocked down PKLR mRNA and protein levels. A miR-130a inhibitor and gRNA increased PKLR expression, HCV replication, and HBV replication, while miR-130a gRNA and PKLR overexpression increased HCV and HBV replication. Supplemental pyruvate increased HCV and HBV replication and rescued the inhibition of HCV and HBV replication by the miR-130a mimic and PKLR knockdown. We concluded that miR-130a regulates HCV and HBV replication through its targeting of PKLR and subsequent pyruvate production. Our data provide novel insights into key metabolic enzymatic pathway steps regulated by miR-130a, including the steps involving PKLR and pyruvate, which are subverted by HCV and HBV replication.IMPORTANCEWe identified that miR-130a regulates the target genePKLRand its subsequent effect on pyruvate production. Pyruvate is a key intermediate in several metabolic pathways, and we identified that pyruvate plays a key role in regulation of HCV and HBV replication. This previously unrecognized, miRNA-regulated antiviral mechanism has implications for the development of host-directed strategies to interrupt the viral life cycle and prevent establishment of persistent infection for HCV, HBV, and potentially other viral infections.

scholarly journals gp180, a Protein That Binds Duck Hepatitis B Virus Particles, Has Metallocarboxypeptidase D-like Enzymatic Activity

1998 ◽  
Vol 273 (14) ◽  
pp. 8382-8388 ◽  
Author(s):  
Francis J. Eng ◽  
Elena G. Novikova ◽  
Kazuyuki Kuroki ◽  
Don Ganem ◽  
Lloyd D. Fricker
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Enzymatic Activity ◽  
Duck Hepatitis B Virus ◽  
Virus Particles ◽  
Duck Hepatitis ◽  
B Virus

scholarly journals gp180, a Host Cell Glycoprotein That Binds Duck Hepatitis B Virus Particles, Is Encoded by a Member of the Carboxypeptidase Gene Family

1995 ◽  
Vol 270 (25) ◽  
pp. 15022-15028 ◽  
Author(s):  
Kazuyuki Kuroki ◽  
Frank Eng ◽  
Takashi Ishikawa ◽  
Christoph Turck ◽  
Fumio Harada ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Gene Family ◽  
Host Cell ◽  
Duck Hepatitis B Virus ◽  
Virus Particles ◽  
Duck Hepatitis ◽  
B Virus

scholarly journals Intracellular single-chain antibody against hepatitis B virus core protein inhibits the replication of hepatitis B virus in cultured cells

Hepatology ◽  
1999 ◽  
Vol 30 (1) ◽  
pp. 300-307 ◽  
Author(s):  
Masato Yamamoto ◽  
Norio Hayashi ◽  
Tetsuo Takehara ◽  
Keiji Ueda ◽  
Eiji Mita ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cultured Cells ◽  
Core Protein ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Virus Core ◽  
B Virus

Identification of an attachment site for human liver plasma membranes on hepatitis B virus particles

Virology ◽  
1989 ◽  
Vol 173 (2) ◽  
pp. 522-530 ◽  
Author(s):  
Patrizia Pontisso ◽  
Maria Grazia Ruvoletto ◽  
Wolfram H. Gerlich ◽  
Klaus-Hinrich Heermann ◽  
Romeo Bardini ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Human Liver ◽  
Plasma Membranes ◽  
Attachment Site ◽  
Virus Particles ◽  
Liver Plasma Membranes ◽  
B Virus
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