Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope

2017 ◽  
pp. 29-44
Author(s):  
André Klauss ◽  
Carsten Hille
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Microscope

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Fiber-optic laser scanning confocal microscope suitable for fluorescence imaging

1994 ◽  
Vol 33 (4) ◽  
pp. 573 ◽  
Author(s):  
Peter M. Delaney ◽  
Martin R. Harris ◽  
Roger G. King
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Fiber Optic ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

3-D Near-Infrared Fluorescence Imaging Using an MEMS-Based Miniature Dual-Axis Confocal Microscope

2009 ◽  
Vol 15 (5) ◽  
pp. 1344-1350 ◽  
Author(s):  
W. Piyawattanametha ◽  
Hyejun Ra ◽  
M.J. Mandella ◽  
K. Loewke ◽  
T.D. Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Confocal Microscope ◽  
Near Infrared Fluorescence ◽  
Near Infrared Fluorescence Imaging

scholarly journals Handheld line-scanned dual-axis confocal microscope with pistoned MEMS actuation for flat-field fluorescence imaging

2019 ◽  
Vol 44 (3) ◽  
pp. 671 ◽  
Author(s):  
Linpeng Wei ◽  
Chengbo Yin ◽  
Yoko Fujita ◽  
Nader Sanai ◽  
Jonathan T. C. Liu
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Flat Field

Reflectance and fluorescence imaging with a MEMS dual-axes confocal microscope

2007 ◽  
Author(s):  
Hyejun Ra ◽  
Wibool Piyawattanametha ◽  
Yoshihiro Taguchi ◽  
Olav Solgaard
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Microscope

scholarly journals Beyond the Hype - Is 2-Photon Microscopy Right for You?

2003 ◽  
Vol 11 (2) ◽  
pp. 26-29
Author(s):  
Jason Kirk
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Imaging Systems ◽  
The Past

Confocal microscopes have come a long way in the past decade. Not only are they more stable and easier to use than ever before, but their cost has dropped enough that multi-user facilities are finding competition from individual labs using the new breed of "personal" confocals. In fact it has, in some cases, become the de facto standard for fluorescence imaging regardless of whether the user actually has requirements for it or not.But, researchers always have an ear out for something better. Enter 2-photon microscopy (2PLSM). The “bigger & badder” cousin of the confocal microscope has become a new weapon in the arsenal of a microscopy industry that caters to researchers who can't wait to fill their labs with the latest and greatest imaging systems. Advertised by the industry and researchers alike as a technique that seems to solve most of the problems that plague confocal, “2-photon” has become the new buzzword in the vocabulary of researchers eager to enhance their fluorescence applications.

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