The influence of confocal microscope design on imaging performance

Author(s):  
C J R Sheppard

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

2021 ◽  
Author(s):  
Domenico Tommasino ◽  
Matteo Bottin ◽  
Giulio Cipriani ◽  
Alberto Doria ◽  
Giulio Rosati

Abstract In robotics the risk of collisions is present both in industrial applications and in remote handling. If a collision occurs, the impact may damage both the robot and external equipment, which may result in successive imprecise robot tasks or line stops, reducing robot efficiency. As a result, appropriate collision avoidance algorithms should be used or, if it is not possible, the robot must be able to react to impacts reducing the contact forces. For this purpose, this paper focuses on the development of a special end-effector that can withstand impacts and is able to protect the robot from impulsive forces. The novel end-effector is based on a bi-stable mechanism that decouples the dynamics of the end-effector from the dynamics of the robot. The intrinsically non-linear behavior of the end-effector is investigated with the aid of numerical simulations. The effect of design parameters and the operating conditions are analyzed and the interaction between the functioning of the bi-stable mechanism and the control system is studied. In particular, the effect of the mechanism in different scenarios characterized by different robot velocities is shown. Results of numerical simulations assess the validity of the proposed end-effector, which can lead to large reductions in impact forces.


2021 ◽  
Author(s):  
Sebastian F. Riebl ◽  
Christian Wakelam ◽  
Reinhard Niehuis

Abstract Turbine Vane Frames (TVF) are a way to realize more compact jet engine designs. Located between the high pressure turbine (HPT) and the low pressure turbine (LPT), they fulfill structural and aerodynamic tasks. When used as an integrated concept with splitters located between the structural load-bearing vanes, the TVF configuration contains more than one type of airfoil with sometimes pronouncedly different properties. This system of multidisciplinary demands and mixed blading poses an interesting opportunity for optimization. Within the scope of the present work, a full geometric parameterization of a TVF with splitters is presented. The parameterization is chosen as to minimize the number of parameters required to automatically and flexibly represent all blade types involved in a TVF row in all three dimensions. Typical blade design parameters are linked to the fourth order Bézier-curve controlled camber line-thickness parameterization. Based on conventional design rules, a procedure is presented, which sets the parameters within their permissible ranges according to the imposed constraints, using a proprietary developed code. The presented workflow relies on subsequent three dimensional geometry generation by transfer of the proposed parameter set to a commercially available CAD package. The interdependencies of parameters are discussed and their respective significance for the adjustment process is detailed. Furthermore, the capability of the chosen parameterization and adjustment process to rebuild an exemplary reference TVF geometry is demonstrated. The results are verified by comparing not only geometrical profile data, but also validated CFD simulation results between the rebuilt and original geometries. Measures taken to ensure the robustness of the method are highlighted and evaluated by exploring extremes in the permissible design space. Finally, the embedding of the proposed method within the framework of an automated, gradient free numerical optimization is discussed. Herein, implications of the proposed method on response surface modeling in combination with the optimization method are highlighted. The method promises to be an option for improvement of optimization efficiency in gradient free optimization of interdependent blade geometries, by a-priori excluding unsuitable blade combinations, yet keeping restrictions to the design space as limited as possible.


Radiology ◽  
2004 ◽  
Vol 233 (2) ◽  
pp. 515-522 ◽  
Author(s):  
Marilyn J. Siegel ◽  
Bernhard Schmidt ◽  
David Bradley ◽  
Christoph Suess ◽  
Charles Hildebolt

2011 ◽  
Vol 04 (03) ◽  
pp. 309-323 ◽  
Author(s):  
CHAO-WEI CHEN ◽  
YU CHEN

Laminar optical tomography (LOT) is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography (DOT). Fluorescence LOT (FLOT) provides depth-resolved molecular information with 100–200 μm resolution over 2–3 mm depth. In this study, we use Monte Carlo simulation and singular-value analysis (SVA) to optimize the source-detector configurations for potential enhancement of FLOT imaging performance. The effects of different design parameters, including source incidence and detector collection angles, detector number, and sampling density, are presented. The results indicate that angled incidence/detection configuration might improve the imaging resolution and depth sensitivity, especially for low-scattering medium. Increasing the number of detectors and the number of scanning steps will also result in enhanced imaging performance. We also demonstrate that the optimal imaging performance depends upon the background scattering coefficient. Our result might provide an optimization strategy for FLOT or LOT experimental setup.


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