C4-O-04Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics

Microscopy ◽  
2015 ◽  
Vol 64 (suppl 1) ◽  
pp. i71.1-i71
Author(s):  
Yasushi Okada
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Spinning Disk

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Rapid Frequency-Domain FLIM Spinning Disk Confocal Microscope: Lifetime Resolution, Image Improvement and Wavelet Analysis

2008 ◽  
Vol 18 (5) ◽  
pp. 929-942 ◽  
Author(s):  
Chittanon Buranachai ◽  
Daichi Kamiyama ◽  
Akira Chiba ◽  
Benjamin D. Williams ◽  
Robert M. Clegg
Keyword(s):  
Wavelet Analysis ◽  
Frequency Domain ◽  
Confocal Microscope ◽  
Resolution Image ◽  
Spinning Disk ◽  
Image Improvement

scholarly journals Full developmental potential of mammalian preimplantation embryos is maintained after imaging using a spinning-disk confocal microscope

BioTechniques ◽  
2006 ◽  
Vol 41 (6) ◽  
pp. 741-750 ◽  
Author(s):  
Pablo Juan Ross ◽  
Gloria Ines Perez ◽  
Tak Ko ◽  
Myung Sik Yoo ◽  
Jose Bernardo Cibelli
Keyword(s):  
Confocal Microscope ◽  
Preimplantation Embryos ◽  
Developmental Potential ◽  
Spinning Disk

Fiber-optic laser scanning confocal microscope suitable for fluorescence imaging

1994 ◽  
Vol 33 (4) ◽  
pp. 573 ◽  
Author(s):  
Peter M. Delaney ◽  
Martin R. Harris ◽  
Roger G. King
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Fiber Optic ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

scholarly journals Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics

2015 ◽  
Vol 26 (9) ◽  
pp. 1743-1751 ◽  
Author(s):  
Shinichi Hayashi ◽  
Yasushi Okada
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Rotating Disk ◽  
Confocal Microscope ◽  
Diffraction Limit ◽  
Live Cell ◽  
Wide Field ◽  
Structured Illumination ◽  
Stripe Pattern ◽  
Spinning Disk

Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumination microscope (SIM) and achieves a spatial resolution of 120 nm, double that of the diffraction limit of wide-field fluorescence microscopy. However, the SDSRM is 10 times faster than a conventional SIM because SR signals are recovered by optical demodulation through the stripe pattern of the disk. Therefore a single SR image requires only a single averaged image through the rotating disk. On the basis of this theory, we modified a commercial spinning disk confocal microscope. The improved resolution around 120 nm was confirmed with biological samples. The rapid dynamics of micro­tubules, mitochondria, lysosomes, and endosomes were observed with temporal resolutions of 30–100 frames/s. Because our method requires only small optical modifications, it will enable an easy upgrade from an existing spinning disk confocal to a SR microscope for live-cell imaging.

Single-cell resolution fluorescence imaging of circadian rhythms detected with a Nipkow spinning disk confocal system

2012 ◽  
Vol 207 (1) ◽  
pp. 72-79 ◽  
Author(s):  
Ryosuke Enoki ◽  
Daisuke Ono ◽  
Mazahir T. Hasan ◽  
Sato Honma ◽  
Ken-ichi Honma
Keyword(s):  
Circadian Rhythms ◽  
Single Cell ◽  
Fluorescence Imaging ◽  
Spinning Disk

3-D Near-Infrared Fluorescence Imaging Using an MEMS-Based Miniature Dual-Axis Confocal Microscope

2009 ◽  
Vol 15 (5) ◽  
pp. 1344-1350 ◽  
Author(s):  
W. Piyawattanametha ◽  
Hyejun Ra ◽  
M.J. Mandella ◽  
K. Loewke ◽  
T.D. Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Confocal Microscope ◽  
Near Infrared Fluorescence ◽  
Near Infrared Fluorescence Imaging
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