Practical Advice on the One-Step Growth Curve

2017 ◽  
pp. 41-47 ◽  
Author(s):  
Andrew M. Kropinski
Keyword(s):  
Growth Curve ◽  
Practical Advice ◽  
One Step ◽  
Step Growth ◽  
The One

Stepping-stones to One-step Growth: Frank Macfarlane Burnet's Role in Elucidating the Viral Nature of the Bacteriophages

2008 ◽  
Vol 19 (1) ◽  
pp. 83 ◽  
Author(s):  
Neeraja Sankaran
Keyword(s):  
Growth Pattern ◽  
Stepping Stones ◽  
Bacterial Origin ◽  
Considerable Debate ◽  
One Step ◽  
Viral Nature ◽  
Step Growth ◽  
The One ◽  
Phage Growth

The demonstration of the one-step growth pattern of the bacteriophages is generally regarded as the key evidence that bacteriophages were viruses rather than enzymes of bacterial origin, a matter of considerable debate among scientists since the bacteriophage was first described in 1917. While the credit for this demonstration is usually accorded to a 1939 paper on phage growth by Emory Ellis and Max Delbr�ck, closer scrutiny of phage research conducted in the intervening two decades reveals that these papers did not present a new idea, but rather extended and refined a line of investigation about the phages that had its conceptual antecedents in the earlier work. Of particular note is the work of the Australian, Frank Macfarlane Burnet, during the late 1920s and early 1930s. Burnet's work also furnished other important reasons besides one-step growth—derived from experiments on lysogeny—for favouring the virus theoryand discarding the enzyme theory of phage. This paper examines Burnet's contributions towards understanding of the nature of phage and makes the case that it was a tacit acceptance of the evidence and arguments that he presented that allowed Ellis and Delbr�ck to make assumptions about the bacteriophage, presented as fact in their papers.

scholarly journals Characterization of RNA synthesis during a one-step growth curve and of the replication mechanism of bovine viral diarrhoea virus

1996 ◽  
Vol 77 (11) ◽  
pp. 2729-2736 ◽  
Author(s):  
Y. Gong ◽  
R. Trowbridge ◽  
T. B. Macnaughton ◽  
E. G. Westaway ◽  
A. D. Shannon ◽  
...  
Keyword(s):  
Growth Curve ◽  
Rna Synthesis ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Replication Mechanism ◽  
Diarrhoea Virus ◽  
One Step ◽  
Step Growth

scholarly journals A Lytic Yersina pestis Bacteriophage Obtained From the Bone Marrow of Marmota himalayana in a Plague-Focus Area in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Junrong Liang ◽  
Shuai Qin ◽  
Ran Duan ◽  
Haoran Zhang ◽  
Weiwei Wu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sequence Similarity ◽  
Tibet Plateau ◽  
Highly Pathogenic ◽  
Serotype O ◽  
Short Latent Period ◽  
One Step ◽  
Step Growth ◽  
The One ◽  
Qinghai Tibet Plateau

A lytic Yersinia pestis phage vB_YpP-YepMm (also named YepMm for briefly) was first isolated from the bone marrow of a Marmota himalayana who died of natural causes on the Qinghai-Tibet plateau in China. Based on its morphologic (isometric hexagonal head and short non-contractile conical tail) and genomic features, we classified it as belonging to the Podoviridae family. At the MOI of 10, YepMm reached maximum titers; and the one-step growth curve showed that the incubation period of the phage was about 10 min, the rise phase was about 80 min, and the lysis amount of the phage during the lysis period of 80 min was about 187 PFU/cell. The genome of the bacteriophage YepMm had nucleotide-sequence similarity of 99.99% to that of the Y. pestis bacteriophage Yep-phi characterized previously. Analyses of the biological characters showed that YepMm has a short latent period, strong lysis, and a broader lysis spectrum. It could infect Y. pestis, highly pathogenic bioserotype 1B/O:8 Y. enterocolitica, as well as serotype O:1b Y. pseudotuberculosis—the ancestor of Y. pestis. It could be further developed as an important biocontrol agent in pathogenic Yersinia spp. infection.

Enhanced electrochromic properties of a polypyrrole–indigo carmine–gold nanoparticles nanocomposite

2015 ◽  
Vol 17 (2) ◽  
pp. 1234-1240 ◽  
Author(s):  
L. F. Loguercio ◽  
C. C. Alves ◽  
A. Thesing ◽  
J. Ferreira
Keyword(s):  
Gold Nanoparticles ◽  
Indigo Carmine ◽  
Electrochromic Properties ◽  
One Step ◽  
Step Growth ◽  
The One

Enhanced electrochromic properties of a PPy–IC–Aunanop nanocomposite obtained by the one-step growth of gold nanoparticles and electropolymerization of indigo carmine doped polypyrrole.

Frequency distribution of phage release in the one-step growth experiment

Virology ◽  
1956 ◽  
Vol 2 (1) ◽  
pp. 96-108 ◽  
Author(s):  
Mark H. Adams ◽  
Felix E. Wassermann
Keyword(s):  
Frequency Distribution ◽  
Growth Experiment ◽  
One Step ◽  
Step Growth ◽  
The One

scholarly journals Replication of Simian Virus 40 Deoxyribonucleic Acid: Analysis of the One-Step Growth Cycle

1973 ◽  
Vol 11 (1) ◽  
pp. 98-106 ◽  
Author(s):  
Simone Manteuil ◽  
Jacqueline Pages ◽  
Dominique Stehelin ◽  
Marc Girard
Keyword(s):  
Deoxyribonucleic Acid ◽  
Simian Virus 40 ◽  
Growth Cycle ◽  
Simian Virus ◽  
One Step ◽  
Step Growth ◽  
The One

scholarly journals CHARACTERIZATION OF T4 MUTANTS THAT PARTIALLY SUPPRESS THE INABILITY OF T4rII TO GROW IN LAMBDA LYSOGENS

Genetics ◽  
1976 ◽  
Vol 83 (3) ◽  
pp. 477-487
Author(s):  
Theodore Homyk ◽  
Angel Rodriguez ◽  
Jon Weil
Keyword(s):  
Burst Size ◽  
Personal Communication ◽  
Growth Cycle ◽  
Plaque Morphology ◽  
One Step ◽  
Step Growth ◽  
The One ◽  
Phage Growth ◽  
L1 And L2

ABSTRACT In the course of isolating viable T4 deletions that affect plaque morphology (Homyk and Weil 1974), two closely linked point mutants, sip1 and sip2, were obtained. They map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rII mutants for growth in lambda lysogens. These characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by Freedman and Brenner (1972). In addition, D. Hall (personal communication) has shown that sip1 and sip2 are similar to the mutant farP85, which affects the regulation of a number of early genes (Chace and Hall 1975).—Sip suppression of rII mutants can be demonstrated in one-step growth experiments, even when both rII genes are completely deleted. This indicates that sip mutants do not simply reduce the level of rII gene products required for growth in a lambda lysogen. Instead, they alter the growth cycle so as to partially circumvent the need for any rII products.—Mutations at two other sites, designated L1 and L2, reverse the poor phage growth caused by sip and, in the one case tested, reverse the rII-suppressing ability of sip.

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