Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis

2018 ◽  
pp. 327-335
Author(s):  
Simone Brand ◽  
Yao-Wen Wu
Keyword(s):  
Amino Acid ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Manipulating Unconventional CH-Based Hydrogen Bonding in a Methyltransferase via Noncanonical Amino Acid Mutagenesis

2014 ◽  
Vol 9 (8) ◽  
pp. 1692-1697 ◽  
Author(s):  
Scott Horowitz ◽  
Upendra Adhikari ◽  
Lynnette M. A. Dirk ◽  
Paul A. Del Rizzo ◽  
Ryan A. Mehl ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Amino Acid ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

scholarly journals Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells

2017 ◽  
Vol 8 (10) ◽  
pp. 7211-7217 ◽  
Author(s):  
Yunan Zheng ◽  
Partha Sarathi Addy ◽  
Raja Mukherjee ◽  
Abhishek Chatterjee
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mammalian Cells ◽  
Noncanonical Amino Acids ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

We systematically evaluate potential platforms for site-specifically incorporating two distinct noncanonical amino acids into proteins expressed in mammalian cells with optimal fidelity and efficiency – a technology that will have many enabling applications.

scholarly journals Expanding the Scope of Single- and Double-Noncanonical Amino Acid Mutagenesis in Mammalian Cells Using Orthogonal Polyspecific Leucyl-tRNA Synthetases

Biochemistry ◽  
2017 ◽  
Vol 57 (4) ◽  
pp. 441-445 ◽  
Author(s):  
Yunan Zheng ◽  
Raja Mukherjee ◽  
Melissa A. Chin ◽  
Peter Igo ◽  
Martin J. Gilgenast ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mammalian Cells ◽  
Trna Synthetases ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

2016 ◽  
Vol 12 (6) ◽  
pp. 1746-1749 ◽  
Author(s):  
Yunan Zheng ◽  
Marc J. Lajoie ◽  
James S. Italia ◽  
Melissa A. Chin ◽  
George M. Church ◽  
...  
Keyword(s):  
Amino Acid ◽  
Release Factor ◽  
Amino Acid Incorporation ◽  
E Coli ◽  
Acid Incorporation ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Unconditional deletion of RF1 in a genomically recoded E. coli enables multisite noncanonical amino acid incorporation by UAG suppression.

An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli

2020 ◽  
Vol 28 (20) ◽  
pp. 115662 ◽  
Author(s):  
Claudio Zambaldo ◽  
Minseob Koh ◽  
Fariborz Nasertorabi ◽  
Gye Won Han ◽  
Abhishek Chatterjee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

scholarly journals Programming Post-Translational Control over the Metabolic Labeling of Cellular Proteins with a Noncanonical Amino Acid

2017 ◽  
Vol 6 (8) ◽  
pp. 1572-1583 ◽  
Author(s):  
Emily E. Thomas ◽  
Naresh Pandey ◽  
Sarah Knudsen ◽  
Zachary T. Ball ◽  
Jonathan J. Silberg
Keyword(s):  
Amino Acid ◽  
Translational Control ◽  
Cellular Proteins ◽  
Metabolic Labeling ◽  
Noncanonical Amino Acid
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