An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli

2020 ◽  
Vol 28 (20) ◽  
pp. 115662 ◽  
Author(s):  
Claudio Zambaldo ◽  
Minseob Koh ◽  
Fariborz Nasertorabi ◽  
Gye Won Han ◽  
Abhishek Chatterjee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

scholarly journals Use of β3-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase

2020 ◽  
Vol 209 (2) ◽  
pp. 107435 ◽  
Author(s):  
Giuliano Nigro ◽  
Sophie Bourcier ◽  
Christine Lazennec-Schurdevin ◽  
Emmanuelle Schmitt ◽  
Philippe Marlière ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Methionyl Trna Synthetase ◽  
Acid Substrate ◽  
Amino Acid Substrate

scholarly journals Amino Acid Toxicities of Escherichia coli That Are Prevented by Leucyl-tRNA Synthetase Amino Acid Editing

2007 ◽  
Vol 189 (23) ◽  
pp. 8765-8768 ◽  
Author(s):  
Vrajesh A. Karkhanis ◽  
Anjali P. Mascarenhas ◽  
Susan A. Martinis
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Amino Acid Editing

ABSTRACT Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple nonstandard as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.

A Tryptophanyl-tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis inE. coli

2013 ◽  
Vol 52 (19) ◽  
pp. 5106-5109 ◽  
Author(s):  
Abhishek Chatterjee ◽  
Han Xiao ◽  
Peng-Yu Yang ◽  
Gautam Soundararajan ◽  
Peter G. Schultz
Keyword(s):  
Amino Acid ◽  
Trna Synthetase ◽  
Unnatural Amino Acid ◽  
Unnatural Amino Acid Mutagenesis ◽  
Amino Acid Mutagenesis
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