Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana

2018 ◽  
pp. 47-75 ◽  
Author(s):  
Selene Napsucialy-Mendivil ◽  
Joseph G. Dubrovsky
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Development ◽  
Lateral Root ◽  
Phenotypic Analysis ◽  
Lateral Root Development

scholarly journals Two receptor‐like protein kinases, MUSTACHES and MUSTACHES‐LIKE, regulate lateral root development in Arabidopsis thaliana

2020 ◽  
Vol 227 (4) ◽  
pp. 1157-1173 ◽  
Author(s):  
Qingqing Xun ◽  
Yunzhe Wu ◽  
Hui Li ◽  
Jinke Chang ◽  
Yang Ou ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Kinases ◽  
Root Development ◽  
Lateral Root ◽  
Lateral Root Development

PP2A mediates lateral root development under NaCl-induced osmotic stress throughout auxin redistribution in Arabidopsis thaliana

2012 ◽  
Vol 368 (1-2) ◽  
pp. 591-602 ◽  
Author(s):  
Mauricio Nahuam Chávez-Avilés ◽  
Claudia Lizeth Andrade-Pérez ◽  
Homero Reyes de la Cruz
Keyword(s):  
Arabidopsis Thaliana ◽  
Osmotic Stress ◽  
Root Development ◽  
Lateral Root ◽  
Lateral Root Development ◽  
Auxin Redistribution

scholarly journals Flavonols modulate lateral root emergence by scavenging reactive oxygen species in Arabidopsis thaliana

2020 ◽  
pp. jbc.RA120.014543
Author(s):  
Jordan M. Chapman ◽  
Gloria K. Muday
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Development ◽  
Lateral Root ◽  
Lateral Roots ◽  
Negative Regulator ◽  
Wild Type ◽  
Root Primordia ◽  
Lateral Root Primordia ◽  
Lateral Root Development ◽  
Genes Encoding

Flavonoids are a class of specialized metabolites with subclasses including flavonols and anthocyanins, which have unique properties as antioxidants. Flavonoids modulate plant development, but whether and how they impact lateral root development is unclear. We examined potential roles for flavonols in this process using Arabidopsis thaliana mutants with defects in genes encoding key enzymes in flavonoid biosynthesis. We observed the tt4 and fls1 mutants, which produce no flavonols, have increased lateral root emergence. The tt4 root phenotype was reversed by genetic and chemical complementation. To more specifically define the flavonoids involved, we tested an array of flavonoid biosynthetic mutants, eliminating roles for anthocyanins and the flavonols quercetin and isorhamnetin in modulating root development. Instead, two tt7 mutant alleles, with defects in a branchpoint enzyme blocking quercetin biosynthesis, formed reduced numbers of lateral roots, and tt7-2 had elevated levels of kaempferol. Using a flavonol-specific dye, we observed that in the tt7-2 mutant, kaempferol accumulated within lateral root primordia at higher levels than wild-type. These data are consistent with kaempferol, or downstream derivatives, acting as a negative regulator of lateral root emergence. We examined ROS accumulation using ROS-responsive probes and found reduced fluorescence of a superoxide-selective probe within the primordia of tt7-2 compared to wild type, but not in the tt4 mutant, consistent with opposite effects of these mutants on lateral root emergence. These results support a model in which increased level of kaempferol in the lateral root primordia of tt7-2 reduces superoxide concentration and ROS-stimulated lateral root emergence.

An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana

2019 ◽  
Vol 70 (7) ◽  
pp. 2157-2171 ◽  
Author(s):  
André Soares ◽  
Stefan Niedermaier ◽  
Rosário Faro ◽  
Andreas Loos ◽  
Bruno Manadas ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Development ◽  
Lateral Root ◽  
Aspartic Protease ◽  
Lateral Root Development

scholarly journals Arabidopsis thaliana mitogen-activated protein kinase 6 is involved in seed formation and modulation of primary and lateral root development

2013 ◽  
Vol 65 (1) ◽  
pp. 169-183 ◽  
Author(s):  
J. S. López-Bucio ◽  
J. G. Dubrovsky ◽  
J. Raya-González ◽  
Y. Ugartechea-Chirino ◽  
J. López-Bucio ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Kinase ◽  
Root Development ◽  
Lateral Root ◽  
Mitogen Activated Protein Kinase ◽  
Seed Formation ◽  
Lateral Root Development ◽  
Mitogen Activated Protein

scholarly journals GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana

2005 ◽  
Vol 56 (419) ◽  
pp. 2433-2442 ◽  
Author(s):  
Laurent Laplaze ◽  
Boris Parizot ◽  
Andrew Baker ◽  
Lilian Ricaud ◽  
Alexandre Martinière ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Development ◽  
Lateral Root ◽  
Genetic Manipulation ◽  
Enhancer Trap ◽  
Lateral Root Development ◽  
Enhancer Trap Lines
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