scholarly journals Determination of Sec18-Lipid Interactions by Liposome-Binding Assay

2018 ◽  
pp. 211-220
Author(s):  
Matthew L. Starr ◽  
Rutilio Fratti
Keyword(s):  
Binding Assay ◽  
Lipid Interactions

A Method for the Determination of Sex Hormone Binding Globulin Using Concanavalin A-Sepharose

1992 ◽  
Vol 29 (2) ◽  
pp. 168-171 ◽  
Author(s):  
J A Whittaker ◽  
M L Cawood ◽  
R E Oakey
Keyword(s):  
Concanavalin A ◽  
Binding Assay ◽  
Solid Phase ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Research Use ◽  
Hormone Binding

A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, imprecision and comparison with other methods, is assessed. The assay is simple and robust and is suitable for analysis of samples of plasma or serum for clinical or research use.

scholarly journals A competition binding assay for determination of the inositol (1,4,5)-trisphosphate content of human leucocytes

1990 ◽  
Vol 170 (2) ◽  
pp. 755-762 ◽  
Author(s):  
Peter H. Nibbering ◽  
Timo P.L. Zomerdijk ◽  
Peter J.M. van Haastert ◽  
Ralph van Furth
Keyword(s):  
Binding Assay ◽  
Competition Binding Assay ◽  
Inositol 1,4,5 Trisphosphate ◽  
Competition Binding

Use of Lucifer Yellow VS as a Label in Fluorescent Immunoassays Illustrated by the Determination of Albumin in Serum

1983 ◽  
Vol 20 (4) ◽  
pp. 213-216 ◽  
Author(s):  
M Philip Bailey ◽  
Bernard F Rocks ◽  
Clifford Riley
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Assay ◽  
Lucifer Yellow ◽  
Stokes Shift ◽  
Covalent Bonds ◽  
Mild Conditions ◽  
Sulphydryl Groups

The use of a new label for fluoroimmunoassay is described. Lucifer yellow VS is a highly fluorescent vinyl sulphone dye which, under mild conditions, forms covalent bonds with amino and sulphydryl groups but is extremely stable in water. A large Stokes shift (110 nm) and an emission maximum at 540 nm give Lucifer yellow further advantages over the more commonly used labels. The use of the dye as a label has been demonstrated by developing a heterogeneous fluoroimmunoassay for human serum albumin. The fluoroimmunoassay gave comparable results to those obtained using a less specific colorimetric dye-binding assay (r = 0·97, n = 20). The advantages, limitations, and other potential uses of Lucifer yellow are discussed.

Inhibitor binding assay for angiotensin-converting enzyme.

1984 ◽  
Vol 30 (5) ◽  
pp. 696-700 ◽  
Author(s):  
F Fyhrquist ◽  
I Tikkanen ◽  
C Grönhagen-Riska ◽  
L Hortling ◽  
M Hichens
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Binding Assay ◽  
Enzyme Inhibitor ◽  
Specific Binding ◽  
Biological Fluids ◽  
The Novel ◽  
Converting Enzyme ◽  
Inhibitor Binding ◽  
Standard Curve

Abstract We describe a new principle for the determination of enzymes, here applied to angiotensin-converting enzyme (ACE, EC 3.4.15.1) in human serum. The enzyme inhibitor binding assay is based on specific binding of labeled inhibitor to the active center of the enzyme. Serum (10-15 microL) is incubated with 125I-labeled ACE inhibitor (" 351A ," a p- hydroxybenzamidine derivative of N-(1-carboxy-3-phenylpropyl)-L-lysyl-L-proline) at pH 7.0 at 37 degrees C for 2 h in a non-equilibrated system. Inhibitor bound to ACE is separated by adsorption to coated charcoal, the radioactivity remaining in the supernate is counted, and the ACE value is calculated from a standard curve. Sensitivity for ACE in serum is 200 U/L, corresponding to 5.0 ng of ACE purified from human lung. The coefficient of variation was 3.9% within assay, and 6.4% between assays for normal ACE activities. Correlation with a comparison spectrophotometric method (Am J Med 59: 363-372, 1975) for ACE assay was excellent (r = 0.98) in 59 samples from healthy subjects and from patients with various diseases including active sarcoidosis. The novel assay principle presented here is simple and specific, and can be extended to use with various biological fluids and tissues, and to other enzymes as well.

Sign in / Sign up

Export Citation Format

Share Document