Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry

2018 ◽  
pp. 243-250 ◽  
Author(s):  
Rivka Noelanders ◽  
Kris Vleminckx
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Xenopus Tropicalis ◽  
Cell Cycle Analysis ◽  
Embryonic Brain ◽  
Fluorescent Reporter

Cell Cycle Analysis by Flow Cytometry

2001 ◽  
Author(s):  
Zbigniew Darzynkiewicz
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Cycle Analysis

scholarly journals Cell-Cycle Analysis of Fission Yeast Cells by Flow Cytometry

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e17175 ◽  
Author(s):  
Jon Halvor Jonsrud Knutsen ◽  
Idun Dale Rein ◽  
Christiane Rothe ◽  
Trond Stokke ◽  
Beáta Grallert ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Fission Yeast ◽  
Yeast Cells ◽  
Cell Cycle Analysis

Chapter 21 Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358—Ethidium Bromide Bivariate Flow Cytometry

1994 ◽  
pp. 327-340 ◽  
Author(s):  
Martin Poot ◽  
Holger Hoehn ◽  
Manfred Kubbies ◽  
Angelika Grossmann ◽  
Yuchyau Chen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Ethidium Bromide ◽  
Cell Cycle Analysis

BrdU—Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis

1988 ◽  
Vol 174 (2) ◽  
pp. 309-318 ◽  
Author(s):  
P.S. Rabinovitch ◽  
M. Kubbies ◽  
Y.C. Chen ◽  
D. Schindler ◽  
H. Hoehn
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Cycle Analysis

Utility of Multiparameter Flow Cytometry Immunophenotypic Studies In Patients with Systemic Light Chain (AL) Amyloidosis

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4051-4051
Author(s):  
Bruno Paiva ◽  
María-Belén Vidriales ◽  
Jose J. Perez ◽  
Maria-Consuelo López-Berges ◽  
Ramón García-Sanz ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Flow Cytometry ◽  
Differential Diagnosis ◽  
Plasma Cell ◽  
Light Chain ◽  
Cell Cycle Analysis ◽  
Disease Assessment ◽  
Multiparameter Flow Cytometry ◽  
Al Amyloidosis

Abstract Abstract 4051 Multiparameter flow cytometry (MFC) immunophenotyping has shown to be of value for differential diagnosis and minimal residual disease assessment in multiple myeloma. However, the clinical value of MFC immunophenotyping in other plasma cell disorders (PCD) remains largely unexplored. Systemic light chain (AL) amyloidosis is a rare PCD characterized by the accumulation of monoclonal light chain fragments leading to end-organ damage and short survival. Bone marrow (BM) plasma cell (PC) infiltration in AL is usually low and thus the identification of clonal PC can be often difficult by immunohistochemistry and/or immunofluorescence. In the present study we focused on 34 BM samples sent to our institution with a suspected diagnosis of AL. MFC immunophenotypic studies were performed using the following 4-color combinations of MoAbs (FITC/PE/PerCP-Cy5.5/APC): CD38/CD56/CD19/CD45 (n=34); in addition cy-Kappa/cy-Lambda/CD19/CD38 staining was add to confirm the clonal or polyclonal nature of BMPC in equivocal cases. Ploidy and cell cycle analysis were additionally performed in a subset of cases (n=12/34). From the total 34 cases included in the present study, 28 had a confirmed diagnosis of AL. The remaining 6 cases were finally diagnosed with localized - amyloidoma - (n=2) and familial (n=1) forms of amyloidosis, multiple myeloma-associated amyloid (n=2) and congestive pericarditis (n=1). Interestingly, the presence of clonal PC was detected by MFC in 27 of the 28 (96%) patients with AL; in turn, clonal PC were undetectable in the BM of all cases with localized and familial forms of amyloidosis. The median overall level of PC (M-PC plus N-PC) seen in MFC immunophenotypic analyses of BM samples of the 28 patients with AL was 1.9% (range: 0.1% - 15%), with a significant positive correlation between PC enumerated by MFC and conventional morphology (r=0.5; p=.01). Within the BMPC compartment, the median proportion of clonal PC was of 94% (mean 81% ± 29%); in 6 cases all BMPC were clonal while in the remaining 22 patients residual normal PC persisted (median of normal PC/BMPC 13% ± 31%). The most common aberrant phenotypes were down-regulation of CD19 (92%) and CD45 (83%), followed by overexpression of CD56 (56%) and infra-expression of CD38 (42%). Aneuploidy was only found in 18% of cases, all of them hyperdiploid. Cell cycle analysis showed a median % of S-phase and G2-Mitosis PC of 0.7% and 3.5%, respectively. Concerning patients' outcome, cases with undetectable normal PC (6/28, 21%) had a significantly decreased overall survival (OS) compared to patients with persistent BM normal PC at diagnosis (22/28, 79%) with 3-year OS rates of 0% vs. 59%, respectively (p=.001). In summary, these preliminary data suggests that MFC immunophenotyping investigations may be clinically relevant in patients with suspected amyloidosis for i) differential diagnosis between AL and other forms of amyloidosis and, ii) prognostication of patients with AL according to the presence or absence of baseline persistent normal PC. Disclosures: No relevant conflicts of interest to declare.

Cell Cycle Analysis of Rat Schwann Cells on Chitosan Scaffolds by Flow Cytometry

2013 ◽  
Vol 284-287 ◽  
pp. 46-50 ◽  
Author(s):  
Chuang Yu Lin ◽  
Li Tzu Li ◽  
Wen Ta Su
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Point Of View ◽  
Cell Cycle Analysis ◽  
Regeneration Medicine ◽  
Artificial Graft ◽  
Chitosan Scaffolds ◽  
Cell Material Interactions

The fine combination of biomaterial and essential cells determines a successful artificial graft. With high biocompatibility, chitosan is a choice of materials for regeneration medicine. In the peripheral nervous system, Schwann cells are critical for nerve regeneration. Schwann cells not only help to conduct the nerve pulse but also guide the nerve extension, especially the injured nerve for recovery. Studies showed that chitosan can be a bridge material for damaged nerve regeneration. The interactions between chitosan and Schwann cells may provide important information for designing the chitosan grafts applied in medical applications. For this purpose, the chitoson was made into conduits by lyophilization. The conduit has porous 3D scaffolds and seeded with rat Schwann cells. The harvested cells were labeled with PI fluorescent dye and analyzed with flow cytometry. The results showed that the rates of DNA replication (S-phase) and cell division (G2 phase) of the cells grew on chitosan scaffolds were higher than the ones grew on the plane substrate. This indicates that the cells grew on chitosan scaffolds were more active than those on the plane substrate in cell proliferation, and the biocompatibility of chitosan can be sustained in this quantitative analysis. Therefore, chitosan scaffolds are efficient for cell expansion of rat Schwann cells and may be beneficial for the purpose of tissue engineering. This study proves that cell cycle analysis is a new point of view in disclosing the cell-material interactions.

scholarly journals Optimization of yeast cell cycle analysis and morphological characterization by multispectral imaging flow cytometry

2008 ◽  
Vol 73A (9) ◽  
pp. 825-833 ◽  
Author(s):  
Meredith E. K. Calvert ◽  
Joanne A. Lannigan ◽  
Lucy F. Pemberton
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Yeast Cell ◽  
Multispectral Imaging ◽  
Morphological Characterization ◽  
Yeast Cell Cycle ◽  
Cell Cycle Analysis ◽  
Imaging Flow Cytometry
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