Sequence-Specific End Labeling of Oligonucleotides (SSELO)-Based Microbial Detection

2018 ◽  
pp. 47-56
Author(s):  
Tanja Kostic ◽  
Levente Bodrossy
Keyword(s):  
Microbial Detection

A NASBA on microgel-tethered molecular-beacon microarray for real-time microbial molecular diagnostics

The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Y. Ma ◽  
X. Dai ◽  
T. Hong ◽  
G. B. Munk ◽  
M. Libera
Keyword(s):  
Real Time ◽  
Bloodstream Infection ◽  
Molecular Diagnostics ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Infection Model ◽  
Rna Amplification ◽  
Microbial Detection

Gel-tethered molecular beacons coupled with NASBA RNA amplification enable real-time microbial detection and differentiation in a bloodstream infection model.

scholarly journals Sequencing and Computational Approaches to Identification and Characterization of Microbial Organisms

2013 ◽  
Vol 5 ◽  
pp. BECB.S10886 ◽  
Author(s):  
Brijesh Singh Yadav ◽  
Venkateswarlu Ronda ◽  
Dinesh P. Vashista ◽  
Bhaskar Sharma
Keyword(s):  
Sequence Data ◽  
Microbial Interactions ◽  
Microbial Pathogens ◽  
Nucleotide Sequence Data ◽  
Computational Approaches ◽  
Microbial Detection ◽  
Sequencing Technologies ◽  
Sequencing Platforms ◽  
Identification And Characterization

The recent advances in sequencing technologies and computational approaches are propelling scientists ever closer towards complete understanding of human-microbial interactions. The powerful sequencing platforms are rapidly producing huge amounts of nucleotide sequence data which are compiled into huge databases. This sequence data can be retrieved, assembled, and analyzed for identification of microbial pathogens and diagnosis of diseases. In this article, we present a commentary on how the metagenomics incorporated with microarray and new sequencing techniques are helping microbial detection and characterization.

scholarly journals Detection of Microbial Nitroreductase Activity by Monitoring Exogenous Volatile Organic Compound Production Using HS-SPME-GC-MS

Separations ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 64
Author(s):  
Ryan Thompson ◽  
John D. Perry ◽  
Stephen P. Stanforth ◽  
John R. Dean
Keyword(s):  
Organic Compound ◽  
Volatile Organic Compound ◽  
Pathogenic Bacteria ◽  
Solid Phase ◽  
Detection System ◽  
Gas Chromatography Mass Spectrometry ◽  
Microbial Detection ◽  
Enzyme Substrates ◽  
Volatile Organic ◽  
Nitroreductase Activity

Development of a rapid approach for universal microbial detection is required in the healthcare, food and environmental sectors to aid with medical intervention, food safety and environmental protection. This research investigates the use of enzymatic hydrolysis of a substrate by a microorganism to generate a volatile organic compound (VOC). One such enzyme activity that can be used in this context is nitroreductase as such activity is prevalent across a range of microorganisms. A study was developed to evaluate a panel of 51 microorganisms of clinical interest for their nitroreductase activity. Two enzyme substrates, nitrobenzene and 1-fluoro-2-nitrobenzene, were evaluated for this purpose with evolution, after incubation, of the VOCs aniline and 2-fluoroaniline, respectively. Detection of the VOCs was done using headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) with obtained limits of quantitation (LOQ) of 0.17 and 0.03 µg/mL for aniline and 2-fluoroaniline, respectively. The results indicated that both enzyme substrates were reduced by the same 84.3% of microorganisms producing the corresponding volatile anilines which were detected using HS-SPME-GC-MS. It was found that nitroreductase activity could be detected after 6–8 h of incubation for the selected pathogenic bacteria investigated. This approach shows promise as a rapid universal microbial detection system.

scholarly journals Liver Abscess Caused by Fusobacterium nucleatum: A Case Report

2021 ◽  
Vol 14 (4) ◽  
Author(s):  
Wenhao Wu ◽  
Wenjia Fan ◽  
Zhewen Zhou ◽  
Shouhao Wang ◽  
Chengan Xu ◽  
...  
Keyword(s):  
Liver Abscess ◽  
Pathogenic Bacteria ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Pyogenic Liver Abscess ◽  
Microbial Detection ◽  
Specimen Collection ◽  
First Case ◽  
Positive Rate ◽  
Bacteria Culture

Introduction: Pyogenic liver abscess (PLA) is a serious infectious disease of the liver. PLA caused by Fusobacterium nucleatum is extremely rare. Here we report the first case of liver abscess caused by F. nucleatum in China. Case Presentation: The case was a 34-year-old female patient admitted to the hospital due to high fever. The diagnosis of liver abscess was confirmed by imaging studies and liver puncture. We finally confirmed the pathogen as F. nucleatum by next-generation sequencing (NGS). After the targeted anti-infective treatment, the patient recovered and discharged. Conclusions: As a new microbial detection method, NGS can still help in clinical practice. In addition, to improve the positive rate of anaerobic bacteria culture, we should pay attention to avoid contact with air in the process of specimen collection when the pathogenic bacteria are suspected to be anaerobic bacteria.

scholarly journals Study on microbial detection technology in food safety

2021 ◽  
Vol 233 ◽  
pp. 02029
Author(s):  
Xindi Zhang
Keyword(s):  
Food Safety ◽  
Rapid Development ◽  
Enzyme Linked Immunosorbent Assay ◽  
Safety Testing ◽  
Microbial Detection ◽  
Detection Technology ◽  
Atp Bioluminescence ◽  
Quality Of Food

Economic development has not only led to the steady development of the gross national economy, but also provided a fundamental guarantee for the life of the residents at this stage. However, with the rapid development of economy, people’s attention to hidden safety problems has gradually shifted from big problems to “small details” of food safety. At the same time, in order to reduce the health problems of consumers in the process of eating products, we should start from the source of food, and use microbial technology in the current food safety testing, so as to fundamentally improve the quality of food safety. At present, PCR, impedance, ATP bioluminescence, lamp and enzyme-linked immunosorbent assay are widely used. In this paper, the role of microbial detection technology was described, and the application of microbial detection technology in food safety detection was analyzed in depth, hoping to provide a reference for ensuring food safety through the promotion of microbial detection technology.

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