scholarly journals Identification of Nuclear Receptor Targets by Chromatin Immunoprecipitation in Fatty Liver

2019 ◽  
pp. 179-188
Author(s):  
Natalia Becares ◽  
Inés Pineda-Torra
Keyword(s):  
Fatty Liver ◽  
Nuclear Receptor ◽  
Chromatin Immunoprecipitation ◽  
Receptor Targets

scholarly journals Hepatic Nuclear Receptor Expression Associates with Features of Histology in Pediatric Nonalcoholic Fatty Liver Disease

2018 ◽  
Vol 2 (10) ◽  
pp. 1213-1226 ◽  
Author(s):  
Erin E. Elbel ◽  
Joel E. Lavine ◽  
Michael Downes ◽  
Mark Van Natta ◽  
Ruth Yu ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Nuclear Receptor ◽  
Fatty Liver Disease ◽  
Receptor Expression ◽  
Nonalcoholic Fatty Liver

Analysis of Chromatin–Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation

2014 ◽  
pp. 25-34 ◽  
Author(s):  
Rosaria Benedetti ◽  
Mariarosaria Conte ◽  
Vincenzo Carafa ◽  
Bartolomeo Della Ventura ◽  
Carlo Altucci ◽  
...  
Keyword(s):  
Nuclear Receptor ◽  
Chromatin Immunoprecipitation ◽  
Receptor Interactions

scholarly journals Dax1 Up-Regulates Oct4 Expression in Mouse Embryonic Stem Cells via LRH-1 and SRA

2010 ◽  
Vol 24 (12) ◽  
pp. 2281-2291 ◽  
Author(s):  
Victoria R. Kelly ◽  
Bin Xu ◽  
Rork Kuick ◽  
Ronald J. Koenig ◽  
Gary D. Hammer
Keyword(s):  
Nuclear Receptor ◽  
Transcriptional Activation ◽  
Chromatin Immunoprecipitation ◽  
Target Genes ◽  
Embryonic Stem ◽  
Steroid Receptor ◽  
Orphan Nuclear Receptor ◽  
Steroidogenic Factor 1 ◽  
Oct4 Expression ◽  
Mes Cells

Abstract Dax1 (Nr0b1) is an atypical orphan nuclear receptor that has recently been shown to play a role in mouse embryonic stem (mES) cell pluripotency. Here we describe a mechanism by which Dax1 maintains pluripotency. In steroidogenic cells, Dax1 protein interacts with the NR5A nuclear receptor steroidogenic factor 1 (Nr5a1) to inhibit transcription of target genes. In mES cells, liver receptor homolog 1 (LRH-1, Nr5a2), the other NR5A family member, is expressed, and LRH-1 has been shown to interact with Dax1. We demonstrate by coimmunoprecipitation that Dax1 is, indeed, able to form a complex with LRH-1 in mES cells. Because Dax1 was historically characterized as an inhibitor of steroidogenic factor 1-mediated transcriptional activation, we hypothesized that Dax1 would inhibit LRH-1 action in mES cells. Therefore, we examined the effect of Dax1 on the LRH-1-mediated activation of the critical ES cell factor Oct4 (Pou5f1). Chromatin immunoprecipitation localized Dax1 to the Oct4 promoter at the LRH-1 binding site, and luciferase assays together with Dax1 overexpression and knockdown experiments revealed that, rather than repress, Dax1 accentuated LRH-1-mediated activation of the Oct4 gene. Similar to our previously published studies that defined the RNA coactivator steroid receptor RNA activator as the critical mediator of Dax1 coactivation function, Dax1 augmentation of LRH-1-mediated Oct4 activation is dependent upon steroid receptor RNA activator. Finally, utilizing published chromatin immunoprecipitation data of whole-genome binding sites of LRH-1 and Dax1, we show that LRH-1 and Dax1 commonly colocalize at 288 genes (43% of LRH-1 target genes), many of which are involved in mES cell pluripotency. Thus, our results indicate that Dax1 plays an important role in the maintenance of pluripotency in mES cells through interaction with LRH-1 and transcriptional activation of Oct4 and other genes.

The nuclear receptor PXR gene variants are associated with liver injury in nonalcoholic fatty liver disease

2010 ◽  
Vol 20 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Silvia Sookoian ◽  
Gustavo O. Castaño ◽  
Adriana L. Burgueño ◽  
Tomas Fernández Gianotti ◽  
María Soledad Rosselli ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Injury ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Nuclear Receptor ◽  
Fatty Liver Disease ◽  
Gene Variants ◽  
Nonalcoholic Fatty Liver

scholarly journals An improved method for quantitative ChIP studies of nuclear receptor function

2019 ◽  
Vol 62 (4) ◽  
pp. 169-177
Author(s):  
Ann Louise Hunter ◽  
Natasha Narang ◽  
Matthew Baxter ◽  
David W Ray ◽  
Toryn M Poolman
Keyword(s):  
Nuclear Receptors ◽  
Nuclear Receptor ◽  
Chromatin Immunoprecipitation ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Correct Interpretation ◽  
Receptor Function ◽  
Improved Method ◽  
Chip Data ◽  
Accurate Quantification

Chromatin immunoprecipitation (ChIP) is a valuable tool for the endocrine researcher, providing a means to measure the recruitment of hormone-activated nuclear receptors, for example. However, the technique can be challenging to perform and has multiple experimental steps, risking introduction of error at each. The data produced can be challenging to interpret; several different methods are commonly used for normalising data and thus comparing between conditions. Absolute, sensitive quantification of protein-bound DNA is important for correct interpretation of the data. In addition, such quantification can help the investigator in troubleshooting experiments. Here, we outline a ChIP strategy combining droplet digital PCR for accurate quantification with an internal spike-in control for normalisation. This combination strengthens the reliability of ChIP data and allows the operator to optimise their protocol with greater confidence.

scholarly journals The Schistosoma mansoni nuclear receptor FTZ-F1 maintains esophageal gland function via transcriptional regulation of meg-8.3

2021 ◽  
Vol 17 (12) ◽  
pp. e1010140
Author(s):  
Aracely A. Romero ◽  
Sarah A. Cobb ◽  
Julie N. R. Collins ◽  
Steven A. Kliewer ◽  
David J. Mangelsdorf ◽  
...  
Keyword(s):  
Nuclear Receptor ◽  
Chromatin Immunoprecipitation ◽  
Hormone Receptors ◽  
Transcriptional Profiling ◽  
Systematic Analysis ◽  
Esophageal Gland ◽  
Parasite Survival ◽  
Gland Function ◽  
Exon Gene ◽  
Transcriptional Target

Schistosomes infect over 200 million of the world’s poorest people, but unfortunately treatment relies on a single drug. Nuclear hormone receptors are ligand-activated transcription factors that regulate diverse processes in metazoans, yet few have been functionally characterized in schistosomes. During a systematic analysis of nuclear receptor function, we found that an FTZ-F1-like receptor was essential for parasite survival. Using a combination of transcriptional profiling and chromatin immunoprecipitation (ChIP), we discovered that the micro-exon gene meg-8.3 is a transcriptional target of SmFTZ-F1. We found that both Smftz-f1 and meg-8.3 are required for esophageal gland maintenance as well as integrity of the worm’s head. Together, these studies define a new role for micro-exon gene function in the parasite and suggest that factors associated with the esophageal gland could represent viable therapeutic targets.

scholarly journals Nuclear Receptor-Mediated Alleviation of Alcoholic Fatty Liver by Polyphenols Contained in Alcoholic Beverages

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e87142 ◽  
Author(s):  
Ruiqing Yao ◽  
Akihito Yasuoka ◽  
Asuka Kamei ◽  
Shota Ushiama ◽  
Yoshinori Kitagawa ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Nuclear Receptor ◽  
Alcoholic Beverages ◽  
Alcoholic Fatty Liver

Abstract 2296: Identification of novel nuclear receptor targets in estrogen receptor negative breast cancer

2011 ◽  
Author(s):  
Kyle R. Covington ◽  
Guowei Gu ◽  
Anna Tsimelzon ◽  
Susan Hilsenbeck ◽  
Powel Brown ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Nuclear Receptor ◽  
Receptor Targets ◽  
Estrogen Receptor Negative
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