Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysis

2019 ◽  
pp. 245-253 ◽  
Author(s):  
Christopher W. Sausen ◽  
Cody M. Rogers ◽  
Matthew L. Bochman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Real Time ◽  
Atp Hydrolysis ◽  
Layer Chromatography

Real-Time Image Acquisition for Absorbance Detection and Quantification in Thin-Layer Chromatography

2006 ◽  
Vol 78 (3) ◽  
pp. 905-911 ◽  
Author(s):  
Michael Lancaster ◽  
David M. Goodall ◽  
Edmund T. Bergström ◽  
Sean McCrossen ◽  
Peter Myers
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Real Time ◽  
Image Acquisition ◽  
Absorbance Detection ◽  
Real Time Image ◽  
Layer Chromatography ◽  
Time Image ◽  
Detection And Quantification

Simple and Practical Real-Time Analysis of Solid-Phase Reactions by Thin-Layer Chromatography

Synlett ◽  
2018 ◽  
Vol 29 (11) ◽  
pp. 1430-1436
Author(s):  
Cheng-Chung Wang ◽  
Chia-Hui Wu ◽  
Chun Chen ◽  
Su-Ching Lin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Real Time ◽  
Solid Phase ◽  
Monitoring Method ◽  
Simple Method ◽  
Real Time Analysis ◽  
Solid Phase Reactions ◽  
User Friendly ◽  
Layer Chromatography

Solid-phase synthesis is a practical approach for simplifying the time-consuming and routine purification steps in the preparation of numerous naturally occurring molecules; however, studying such reactions is difficult due to the lack of a convenient monitoring method. By using thin-layer chromatography in conjunction with a photolabile linker on a resin, we developed a convenient and simple method for monitoring solid-phase reactions in real time by thin-layer chromatography. This method provides a user-friendly protocol for examining reaction conditions for solid-state syntheses.

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