Detection of UV-Induced Thymine Dimers

2019 ◽  
pp. 313-322
Author(s):  
Vipin Kumar Yadav ◽  
Poorwa Awasthi ◽  
Amit Kumar
Keyword(s):  
Thymine Dimers

scholarly journals Experimental and theoretical studies on thymine photodimerization mediated by oxidatively generated DNA lesions and epigenetic intermediates

2020 ◽  
Vol 22 (44) ◽  
pp. 25661-25668
Author(s):  
Mauricio Lineros-Rosa ◽  
Antonio Francés-Monerris ◽  
Antonio Monari ◽  
Miguel Angel Miranda ◽  
Virginie Lhiaubet-Vallet
Keyword(s):  
Dna Lesions ◽  
Theoretical Studies ◽  
Computational Studies ◽  
Thymine Dimers ◽  
Different Types ◽  
Experimental And Theoretical Studies

Combined spectroscopic and computational studies reveal that, in spite of their structural similarities, 5-formyluracil and 5-formylcytosine photosensitize cyclobutane thymine dimers through two different types of mechanisms.

scholarly journals Lignin Nanoparticles Deliver Novel Thymine Biomimetic Photo-Adducts with Antimelanoma Activity

2022 ◽  
Vol 23 (2) ◽  
pp. 915
Author(s):  
Sofia Gabellone ◽  
Davide Piccinino ◽  
Silvia Filippi ◽  
Tiziana Castrignanò ◽  
Claudio Zippilli ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Molecular Docking ◽  
Controlled Release ◽  
Selectivity Index ◽  
Docking Analysis ◽  
Topoisomerase 1 ◽  
Thymine Dimers ◽  
Repair Pathways ◽  
Dna Complex ◽  
Molecular Docking Analysis

We report here the synthesis of novel thymine biomimetic photo-adducts bearing an alkane spacer between nucleobases and characterized by antimelanoma activity against two mutated cancer cell lines overexpressing human Topoisomerase 1 (TOP1), namely SKMEL28 and RPMI7951. Among them, Dewar Valence photo-adducts showed a selectivity index higher than the corresponding pyrimidine-(6-4)-pyrimidone and cyclobutane counterpart and were characterized by the highest affinity towards TOP1/DNA complex as evaluated by molecular docking analysis. The antimelanoma activity of novel photo-adducts was retained after loading into UV photo-protective lignin nanoparticles as stabilizing agent and efficient drug delivery system. Overall, these results support a combined antimelanoma and UV sunscreen strategy involving the use of photo-protective lignin nanoparticles for the controlled release of thymine dimers on the skin followed by their sacrificial transformation into photo-adducts and successive inhibition of melanoma and alert of cellular UV machinery repair pathways.

scholarly journals Detection of UV-Induced Thymine Dimers in Individual Cryptosporidium parvum and Cryptosporidium hominis Oocysts by Immunofluorescence Microscopy

2006 ◽  
Vol 73 (3) ◽  
pp. 947-955 ◽  
Author(s):  
B. H. Al-Adhami ◽  
R. A. B. Nichols ◽  
J. R. Kusel ◽  
J. O'Grady ◽  
H. V. Smith
Keyword(s):  
Cryptosporidium Parvum ◽  
Immunofluorescence Microscopy ◽  
Uv Light ◽  
Fluorescein Isothiocyanate ◽  
Immunofluorescence Assay ◽  
Cryptosporidium Hominis ◽  
Specific Fluorescence ◽  
Thymine Dimers ◽  
Texas Red

ABSTRACT To investigate the effect of UV light on Cryptosporidium parvum and Cryptosporidium hominis oocysts in vitro, we exposed intact oocysts to 4-, 10-, 20-, and 40-mJ�cm−2 doses of UV irradiation. Thymine dimers were detected by immunofluorescence microscopy using a monoclonal antibody against cyclobutyl thymine dimers (anti-TDmAb). Dimer-specific fluorescence within sporozoite nuclei was confirmed by colocalization with the nuclear fluorogen 4′,6′-diamidino-2-phenylindole (DAPI). Oocyst walls were visualized using either commercial fluorescein isothiocyanate-labeled anti-Cryptosporidium oocyst antibodies (FITC-CmAb) or Texas Red-labeled anti-Cryptosporidium oocyst antibodies (TR-CmAb). The use of FITC-CmAb interfered with TD detection at doses below 40 mJ�cm−2. With the combination of anti-TDmAb, TR-CmAb, and DAPI, dimer-specific fluorescence was detected in sporozoite nuclei within oocysts exposed to 10 to 40 mJ�cm−2 of UV light. Similar results were obtained with C. hominis. C. parvum oocysts exposed to 10 to 40 mJ�cm−2 of UV light failed to infect neonatal mice, confirming that results of our anti-TD immunofluorescence assay paralleled the outcomes of our neonatal mouse infectivity assay. These results suggest that our immunofluorescence assay is suitable for detecting DNA damage in C. parvum and C. hominis oocysts induced following exposure to UV light.

Excision of thymine dimers from specifically incised DNA by extracts of xeroderma pigmentosum cells

Nature ◽  
1975 ◽  
Vol 256 (5514) ◽  
pp. 235-236 ◽  
Author(s):  
K. COOK ◽  
E. C. FRIEDBERG ◽  
J. E. CLEAVER
Keyword(s):  
Xeroderma Pigmentosum ◽  
Thymine Dimers

PROPERTIES OF THYMINE DIMERS

1969 ◽  
Vol 9 (1) ◽  
pp. 33-43 ◽  
Author(s):  
M. A. Herbert ◽  
J. C. Leblanc ◽  
D. Weinblum ◽  
H. E. Johns
Keyword(s):  
Thymine Dimers
Sign in / Sign up

Export Citation Format

Share Document