Comparative Poly(A)+ RNA Interactome Capture of RNA Surveillance Mutants

2019 ◽  
pp. 255-276 ◽  
Author(s):  
Cornelia Kilchert ◽  
Svenja Hester ◽  
Alfredo Castello ◽  
Shabaz Mohammed ◽  
Lidia Vasiljeva
Keyword(s):  
Rna Surveillance

scholarly journals Noncoding RNA Surveillance: The Ends Justify the Means

2017 ◽  
Vol 118 (8) ◽  
pp. 4422-4447 ◽  
Author(s):  
Cedric Belair ◽  
Soyeong Sim ◽  
Sandra L. Wolin
Keyword(s):  
Noncoding Rna ◽  
Rna Surveillance

scholarly journals A meiotic gene regulatory cascade driven by alternative fates for newly synthesized transcripts

2011 ◽  
Vol 22 (1) ◽  
pp. 66-77 ◽  
Author(s):  
Nicole Cremona ◽  
Kristine Potter ◽  
Jo Ann Wise
Keyword(s):  
Fission Yeast ◽  
Rna Processing ◽  
Yeast Cells ◽  
Forkhead Transcription Factor ◽  
Rna Surveillance ◽  
Regulatory Cascade ◽  
Show Evidence ◽  
Gene Regulatory ◽  
Rna Accumulation ◽  
Temporal Profiles

To determine the relative importance of transcriptional regulation versus RNA processing and turnover during the transition from proliferation to meiotic differentiation in the fission yeast Schizosaccharomyces pombe, we analyzed temporal profiles and effects of RNA surveillance factor mutants on expression of 32 meiotic genes. A comparison of nascent transcription with steady-state RNA accumulation reveals that the vast majority of these genes show a lag between maximal RNA synthesis and peak RNA accumulation. During meiosis, total RNA levels parallel 3′ processing, which occurs in multiple, temporally distinct waves that peak from 3 to 6 h after meiotic induction. Most early genes and one middle gene, mei4, share a regulatory mechanism in which a specialized RNA surveillance factor targets newly synthesized transcripts for destruction. Mei4p, a member of the forkhead transcription factor family, in turn regulates a host of downstream genes. Remarkably, a spike in transcription is observed for less than one-third of the genes surveyed, and even these show evidence of RNA-level regulation. In aggregate, our findings lead us to propose that a regulatory cascade driven by changes in processing and stability of newly synthesized transcripts operates alongside the well-known transcriptional cascade as fission yeast cells enter meiosis.

scholarly journals Autoimmunity as a Result of Escape from RNA Surveillance

2006 ◽  
Vol 177 (3) ◽  
pp. 1698-1707 ◽  
Author(s):  
Michael P. Bachmann ◽  
Holger Bartsch ◽  
Joanne K. Gross ◽  
Shannon M. Maier ◽  
Timothy F. Gross ◽  
...  
Keyword(s):  
Rna Surveillance

scholarly journals COVID-19 in a Rural Community: Outbreak Dynamics, Contact Tracing and Environmental RNA

2020 ◽  
Author(s):  
Isabel G. Fernández de Mera ◽  
Francisco J. Rodríguez del Río ◽  
José de la Fuente ◽  
Marta Pérez Sancho ◽  
Dolores Hervas ◽  
...  
Keyword(s):  
Rural Community ◽  
Public Service ◽  
Contact Tracing ◽  
Rt Pcr ◽  
Rna Surveillance ◽  
Petrol Station ◽  
Early Testing ◽  
Resource Deployment ◽  
The Village ◽  
Close Contacts

Background: Since March 2020, Spain is severely hit by the ongoing pandemic of coronavirus disease 19 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Understanding and disrupting the early transmission dynamics of the infection is crucial for impeding sustained transmission. Methods: We recorded all COVID-19 cases and traced their contacts in an isolated rural community. We also sampled 10 households, 6 public service sites and the wastewater from the village sewage for environmental SARS-CoV-2 RNA. Results: The first village patient diagnosed with COVID-19-compatible symptoms occurred on March 3, 2020, twelve days before lockdown. A peak of 39 cases occurred on March 30. By May 15, the accumulated number of symptomatic cases was 53 (6% of the population), of which only 22 (41%) had been tested and confirmed by RT-PCR as SARS-CoV-2 infected, including 16 hospitalized patients. Contacts (n=144) were six times more likely to develop symptoms. Environmental sampling detected SARS-CoV-2 RNA in two households with known active cases and in two public service sites: the petrol station and the pharmacy. Samples from other sites and the wastewater tested negative. Conclusions: The low proportion of patients tested by RT-PCR calls for urgent changes in disease management. We propose that early testing of all cases and their close contacts would reduce infection spread, reducing the disease burden and fatalities. In a context of restricted testing, environmental RNA surveillance might prove useful for early warning and to identify high-risk settings enabling a targeted resource deployment.

scholarly journals RNA surveillance by uridylation-dependent RNA decay inSchizosaccharomyces pombe

2019 ◽  
Vol 47 (6) ◽  
pp. 3045-3057 ◽  
Author(s):  
Christina Z Chung ◽  
Julia E Jaramillo ◽  
Michael J Ellis ◽  
Daniel Y N Bour ◽  
Lauren E Seidl ◽  
...  
Keyword(s):  
Rna Decay ◽  
Rna Surveillance

scholarly journals Biology of RNA Surveillance in Development and Disease

2019 ◽  
Vol 29 (5) ◽  
pp. 428-445 ◽  
Author(s):  
Brice Laffleur ◽  
Uttiya Basu
Keyword(s):  
Rna Surveillance
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