Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)

ABSTRACTSapovirus(SaV), from theCaliciviridaefamily, is a genus of enteric viruses that cause acute gastroenteritis. SaV is shed at high concentrations with feces into wastewater, which is usually discharged into aquatic environments or reused for irrigation without efficient treatments. This study analyzed the incidence of human SaV in four wastewater treatment plants from Tunisia during a period of 13 months (December 2009 to December 2010). Detection and quantification were carried out using reverse transcription-quantitative PCR (RT-qPCR) methods, obtaining a prevalence of 39.9% (87/218). Sixty-one positive samples were detected in untreated water and 26 positive samples in processed water. The Dekhila plant presented the highest contamination levels, with a 63.0% prevalence. A dominance of genotype I.2 was observed on 15 of the 24 positive samples that were genetically characterized. By a Bayesian estimation algorithm, the SaV density in wastewater was estimated using left-censored data sets. The mean value of log SaV concentration in untreated wastewater ranged between 2.7 and 4.5 logs. A virus removal efficiency of 0.2 log was calculated for the Dekhila plant as the log ratio posterior distributions between untreated and treated wastewater. Multiple quantitative values obtained in this study must be available in quantitative microbial risk assessment in Tunisia as parameter values reflecting local conditions.IMPORTANCEHuman sapovirus (SaV) is becoming more prevalent worldwide and organisms in this genus are recognized as emerging pathogens associated with human gastroenteritis. The present study describes novel findings on the prevalence, seasonality, and genotype distribution of SaV in Tunisia and Northern Africa. In addition, a statistical approximation using Bayesian estimation of the posterior predictive distribution (“left-censored” data) was employed to solve methodological problems related with the limit of quantification of the quantitative PCR (qPCR). This approach would be helpful for the future development of quantitative microbial risk assessment procedures for wastewater.

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Reduction of Arcobacter at Two Conventional Wastewater Treatment Plants in Southern Arizona, USA

Pathogens ◽

10.3390/pathogens8040175 ◽

2019 ◽

Vol 8 (4) ◽

pp. 175 ◽

Cited By ~ 4

Author(s):

Ghaju Shrestha ◽

Sherchan ◽

Kitajima ◽

Tanaka ◽

Gerba ◽

...

Keyword(s):

Wastewater Treatment ◽

16S Rrna ◽

16S Rrna Gene ◽

Quantitative Pcr ◽

Pathogenic Bacteria ◽

Wastewater Treatment Plants ◽

The United States ◽

Rrna Gene ◽

Metagenomic Sequencing ◽

Wwtp Effluent

This study aimed to identify the bacterial community in two wastewater treatment plants (WWTPs) and to determine the occurrence and reduction of Arcobacter, along with virulence genes (ciaB and pldA). A total of 48 samples (24 influent and 24 effluent) were collected at two WWTPs in southern Arizona in the United States, monthly from August 2011 to July 2012. Bacterial DNA extract was utilized for 16S rRNA metagenomic sequencing. Quantification of Arcobacter 16S rRNA gene was conducted using a recently developed SYBR Green-based quantitative PCR assay. Among 847 genera identified, 113 (13%) were identified as potentially pathogenic bacteria. Arcobacter 16S rRNA gene was detected in all influent samples and ten (83%) and nine (75%) effluent samples at each plant, respectively. Log reduction ratios of Arcobacter 16S rRNA gene in Plant A and Plant B were 1.7 ± 0.9 (n = 10) and 2.3 ± 1.5 (n = 9), respectively. The ciaB gene was detected by quantitative PCR in eleven (92%) and twelve (100%) of 12 influent samples from Plant A and Plant B, respectively, while the pldA gene was detected in eight (67%) and six (50%) influent samples from Plant A and Plant B, respectively. The prevalence of potentially pathogenic bacteria in WWTP effluent indicated the need for disinfection before discharge into the environment.

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Quantification of viable bacteria in wastewater treatment plants by using propidium monoazide combined with quantitative PCR (PMA-qPCR)

Journal of Environmental Sciences ◽

10.1016/s1001-0742(13)60425-8 ◽

2014 ◽

Vol 26 (2) ◽

pp. 299-306 ◽

Cited By ~ 27

Author(s):

Dan Li ◽

Tiezheng Tong ◽

Siyu Zeng ◽

Yiwen Lin ◽

Shuxu Wu ◽

...

Keyword(s):

Wastewater Treatment ◽

Quantitative Pcr ◽

Wastewater Treatment Plants ◽

Propidium Monoazide ◽

Viable Bacteria

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Analysis and balance of phosphorous removal from wastewater at urban wastewater treatment plant

Pollack Periodica ◽

10.1556/606.2020.15.2.13 ◽

2020 ◽

Vol 15 (2) ◽

pp. 142-151

Author(s):

Peter Lukac ◽

Lubos Jurik

Keyword(s):

Wastewater Treatment ◽

Wastewater Treatment Plant ◽

Wastewater Treatment Plants ◽

Sewage Treatment ◽

Sewage Treatment Plant ◽

Treatment Plant ◽

Phosphorus Recovery ◽

Anaerobic Sludge ◽

Biological Phosphorus Removal ◽

The Mean

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.

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