Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device

2019 ◽  
pp. 107-111
Author(s):  
Barbara J. Stone
Keyword(s):  
Embryo Transfer ◽  
Transfer Protocol

scholarly journals NEONATAL AND MATERNAL OUTCOMES IN SINGLETON LIVE BIRTHS (LB) FOLLOWING SINGLE EUPLOID FROZEN EMBRYO TRANSFER (FET): DOES TRANSFER PROTOCOL MATTER?

2020 ◽  
Vol 114 (3) ◽  
pp. e311
Author(s):  
Hilary S. Friedlander ◽  
Paxton E. Voigt ◽  
Jennifer K. Blakemore ◽  
James A. Grifo
Keyword(s):  
Embryo Transfer ◽  
Frozen Embryo Transfer ◽  
Maternal Outcomes ◽  
Live Births ◽  
Transfer Protocol

115 FOLLICULAR GROWTH AND BLOOD FLOW OF THE DOMINANT FOLLICLE IN CROSSBRED RECIPIENTS TREATED WITH eCG

2014 ◽  
Vol 26 (1) ◽  
pp. 171
Author(s):  
M. P. Palhao ◽  
N. S. Junior ◽  
C. R. B. Guimarães ◽  
C. A. C. Fernandes ◽  
M. E. O. Ferreira ◽  
...  
Keyword(s):  
Blood Flow ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Sao Paulo ◽  
Ovulation Rate ◽  
São Paulo ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Follicle Diameter ◽  
Transfer Protocol

This study aimed to explore changes in follicle diameter and blood flow of the dominant follicle (DF), in ovulation and embryo transfer rates, after inclusion of eCG in a protocol for timed embryo transfer. The effect presence or absence of a corpus luteum (CL) at the start of treatment was also included. Crossbred heifers (n = 116, Bos taurus × Bos indicus), with (n = 61) or without (n = 55) CL, were included in the same hormone protocol: Day 0 (D0), insertion of progesterone (P4) device (1.0 g, Sincrogest®, Ouro Fino, São Paulo, Brazil) and 2 mg of oestradiol benzoato (EB, Sincrodiol®, Ouro Fino); D8, removal of P4 device and injection of sodium Cloprostenol (0.250 mg mL–1, Sincrocio®, Ouro Fino). On D8, the animals with and without CL – at the beginning of the protocol – were equally divided into 2 groups (G): G1 – injection of 300 IU (2.0 mL) of eCG (n = 56; Synchro eCG®, Ouro Fino); G2 – 2.0 mL of saline (n = 60). The ovulations were synchronized with 1 mg of EB on D9. From D8 to D11, the diameter of the DF and blood flow in its wall were recorded daily (M5 ultrasound with colour Doppler technology, 7.5-MHz linear array, DPS medical equipment, São Paulo, Brazil). Approximately 100 frames in colour-flow mode, containing entire cross-sections of the DF, were recorded during each examination. The area of the follicular wall with coloured pixels was measured with ImageJ software (Image Processing and Analysis in Java) from the frame with the largest blood flow signal. Before embryo transfer, all heifers were evaluated, and those with good-quality CL received frozen/thawed embryos (ethylene glycol 1.5 mol). Follicle diameter and blood flow area were compared between groups with or without CL before timed embryo transfer protocol and between eCG treatments. The PROC GLM procedure of SAS (version 9.0) and the t-test were used to assess the differences between means. Pregnancy diagnosis was performed on D35. Embryo transfer (ET) rate of the recipients and pregnancy rate were compared between CL or eCG treatments by the chi-squared test. Ovarian status, before hormone protocol, did not change (P > 0.05) the follicular growth of the DF. However, ovulation rate (78.8 v. 65.4%, P < 0.05) and ET rate (78.7 v. 65.4%, P < 0.05) were higher in animals with CL on D0. From D8 to D10, the inclusion of eCG did not affect (P > 0.05) follicular growth and blood flow of the DF. The time effect (P < 0.0001) for follicular blood flow had shown an increase in area of blood flow 24 h after implant removal (7.7 ± 0.7,b 10.2 ± 0.7,a and 12.3 ± 1.0a mm2, for Days 8, 9, and 10, respectively). The eCG did not affect (P > 0.05) the ovulation rate (71.4 and 73.3%, respectively, eCG and no eCG), however, approached an increased (P < 0.06) ET rate (78.8 v. 66.7%). The overall pregnancy rate (51.2%, 43/84) was not affected (P > 0.05) by evaluated variables. In summary, the addition of 300 IU of eCG on D8 of the timed embryo transfer protocol did not change the development of DF but increased the ET rate of the recipients. Biotran, FAPEMIG (project number APQ-1454-12), and CnPQ are acknowledged.

Evaluation of the embryo transfer protocol by a laboratory model of the uterus

2007 ◽  
Vol 88 (2) ◽  
pp. 485-493 ◽  
Author(s):  
Osnat Eytan ◽  
David Elad ◽  
Ariel J. Jaffa
Keyword(s):  
Embryo Transfer ◽  
Laboratory Model ◽  
Transfer Protocol

scholarly journals A CIDR-Based Timed Embryo Transfer Protocol Increases the Pregnancy Rate of Lactating Repeat Breeder Dairy Cows

2007 ◽  
Vol 53 (6) ◽  
pp. 1313-1318 ◽  
Author(s):  
Dong-Soo SON ◽  
Chang-Yong CHOE ◽  
Sang-Rae CHO ◽  
Sun-Ho CHOI ◽  
Hyun-Jong KIM ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Repeat Breeder ◽  
Transfer Protocol

scholarly journals ASRM standard embryo transfer protocol template: a committee opinion

2017 ◽  
Vol 107 (4) ◽  
pp. 897-900 ◽  
Author(s):  
Alan Penzias ◽  
Kristin Bendikson ◽  
Samantha Butts ◽  
Christos Coutifaris ◽  
Tommaso Falcone ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Transfer Protocol

Transfer of 2 Embryos Using a Double-Embryo Transfer Protocol Versus 2 Sequential Single-Embryo Transfers: The Impact on Multiple Pregnancy

2018 ◽  
Vol 25 (10) ◽  
pp. 1501-1508 ◽  
Author(s):  
Pedro A. A. Monteleone ◽  
Pedro F. M. Peregrino ◽  
Edmund C. Baracat ◽  
Paulo C. Serafini
Keyword(s):  
Embryo Transfer ◽  
Multiple Pregnancy ◽  
The Impact ◽  
Double Embryo Transfer ◽  
Transfer Protocol

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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