scholarly journals Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation

2010 ◽  
pp. 317-327 ◽  
Author(s):  
Jing-Ruey J. Yeh ◽  
Kathleen M. Munson
Keyword(s):  
Cell Fate ◽  
Small Molecule ◽  
Small Molecule Screen

scholarly journals Small-Molecule Screen Identifies De Novo Nucleotide Synthesis as a Vulnerability of Cells Lacking SIRT3

Cell Reports ◽  
2018 ◽  
Vol 22 (8) ◽  
pp. 1945-1955 ◽  
Author(s):  
Karina N. Gonzalez Herrera ◽  
Elma Zaganjor ◽  
Yoshinori Ishikawa ◽  
Jessica B. Spinelli ◽  
Haejin Yoon ◽  
...  
Keyword(s):  
Small Molecule ◽  
De Novo ◽  
Nucleotide Synthesis ◽  
Small Molecule Screen

scholarly journals Small molecule screen for compounds that affect vascular development in the zebrafish retina

2009 ◽  
Vol 126 (5-6) ◽  
pp. 464-477 ◽  
Author(s):  
Satish S. Kitambi ◽  
Kyle J. McCulloch ◽  
Randall T. Peterson ◽  
Jarema J. Malicki
Keyword(s):  
Small Molecule ◽  
Vascular Development ◽  
Small Molecule Screen

scholarly journals A small molecule screen identified N-Acetyl-L-cysteine for promoting ex vivo growth of single circulating tumor cells

2020 ◽  
Author(s):  
Teng Teng ◽  
Mohamed Kamal ◽  
Oihana Iriondo ◽  
Yonatan Amzaleg ◽  
Chunqiao Luo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Small Molecule ◽  
Ex Vivo ◽  
Single Cells ◽  
Small Subset ◽  
Blood Draw ◽  
Small Molecule Screen ◽  
Functional Analyses ◽  
Single Ctcs

AbstractCirculating tumor cells (CTCs) can be isolated via a minimally invasive blood draw and are considered a “liquid biopsy” of their originating solid tumors. CTCs contain a small subset of metastatic precursors that can form metastases in secondary organs, and provide a resource to identify mechanisms underlying metastasis-initiating properties. Despite technological advancements that allow for highly sensitive approaches of detection and isolation, CTCs are very rare and often present as single cells, posing an extreme challenge for ex vivo expansion after isolation. Here, using previously established patient-derived CTC lines, we performed a small molecule drug screening to identify compounds that can improve ex vivo culture efficiency for single CTCs. We found that N-acetylcysteine (NAC) and other antioxidants can promote ex vivo expansion of single CTCs, by reducing oxidative and other stress particularly at the initial stage of single cell expansion. RNA-seq analysis of growing clones and non-growing clones confirmed the effect by NAC, but also indicate that NAC-induced decrease in oxidative stress is insufficient for promoting proliferation of a subset of cells with heterogeneous quiescent and senescent features. Despite the challenge in expanding all CTCs, NAC treatment lead to establishment of single CTC clones that have similar tumorigenic features, which will facilitate future functional analyses.

scholarly journals High-Throughput Small Molecule Screen Identifies Modulators of Mitochondrial Function in Neurons

iScience ◽  
2020 ◽  
Vol 23 (8) ◽  
pp. 101364
Author(s):  
Boglarka H. Varkuti ◽  
Ze Liu ◽  
Miklos Kepiro ◽  
Rodrigo Pacifico ◽  
Yunchao Gai ◽  
...  
Keyword(s):  
Small Molecule ◽  
High Throughput ◽  
Mitochondrial Function ◽  
Small Molecule Screen

A small-molecule screen in C. elegans yields a new calcium channel antagonist

Nature ◽  
2006 ◽  
Vol 441 (7089) ◽  
pp. 91-95 ◽  
Author(s):  
Trevor C. Y. Kwok ◽  
Nicole Ricker ◽  
Regina Fraser ◽  
Allen W. Chan ◽  
Andrew Burns ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Small Molecule ◽  
Calcium Channel Antagonist ◽  
C Elegans ◽  
Small Molecule Screen

scholarly journals A Small-molecule Screen Yields Idiotype-specific Blockers of Neuromyelitis Optica Immunoglobulin G Binding to Aquaporin-4

2012 ◽  
Vol 287 (44) ◽  
pp. 36837-36844 ◽  
Author(s):  
Puay-Wah Phuan ◽  
Marc O. Anderson ◽  
Lukmanee Tradtrantip ◽  
Hua Zhang ◽  
Joseph Tan ◽  
...  
Keyword(s):  
Small Molecule ◽  
Neuromyelitis Optica ◽  
Immunoglobulin G ◽  
Aquaporin 4 ◽  
Small Molecule Screen

scholarly journals A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

10.3791/52063 ◽  
2014 ◽  
Author(s):  
Shahram Jevin Poureetezadi ◽  
Eric K. Donahue ◽  
Rebecca A. Wingert
Keyword(s):  
Small Molecule ◽  
High Throughput ◽  
Zebrafish Embryos ◽  
Small Molecule Screen

Pluripotent Stem Cells Induced from Mouse Somatic Cells by Small-Molecule Compounds

Science ◽  
2013 ◽  
Vol 341 (6146) ◽  
pp. 651-654 ◽  
Author(s):  
Pingping Hou ◽  
Yanqin Li ◽  
Xu Zhang ◽  
Chun Liu ◽  
Jingyang Guan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Fate ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Genetic Manipulation ◽  
Expression Profiles ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Gene Expression Profiles ◽  
Clinical Applications

Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous “master genes” are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.

scholarly journals A Small-Molecule Screen for Enhanced Homing of Systemically Infused Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. 1261-1268 ◽  
Author(s):  
Oren Levy ◽  
Luke J. Mortensen ◽  
Gerald Boquet ◽  
Zhixiang Tong ◽  
Christelle Perrault ◽  
...  
Keyword(s):  
Small Molecule ◽  
Small Molecule Screen
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