scholarly journals Chromatin Immunoprecipitation Assay as a Tool for Analyzing Transcription Factor Activity

2011 ◽  
pp. 85-104 ◽  
Author(s):  
Padmaja Gade ◽  
Dhan V. Kalvakolanu
Keyword(s):  
Transcription Factor ◽  
Chromatin Immunoprecipitation ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Chromatin Immunoprecipitation Assay ◽  
Immunoprecipitation Assay

Modulation of the Escherichia coli σ E (RpoE) heat‐shock transcription‐factor activity by the RseA, RseB and RseC proteins

1997 ◽  
Vol 24 (2) ◽  
pp. 355-371 ◽  
Author(s):  
Dominique Missiakas ◽  
Matthias P. Mayer ◽  
Marc Lemaire ◽  
Costa Georgopoulos ◽  
Satish Raina
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Heat Shock ◽  
Heat Shock Transcription Factor ◽  
Transcription Factor Activity ◽  
Factor Activity

scholarly journals Effect of aging on UVC-induced apoptosis of rat splenocytes.

2000 ◽  
Vol 47 (2) ◽  
pp. 339-347 ◽  
Author(s):  
E Radziszewska ◽  
K Piwocka ◽  
A Bielak-Zmijewska ◽  
J Skierski ◽  
E Sikora
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Morphological Changes ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Dna Ladder ◽  
Young Rats ◽  
Bax Protein ◽  
Old Rats ◽  
Induced Apoptosis

UVC-induced apoptotic symptoms such as morphological changes, DNA fragmentation, Bcl-2 and Bax protein expression were examined in primary splenocyte cultures from young (3 months) and old (24 months) rats. The activities of AP-1 and CRE transcription factors in UVC-irradiated splenocytes were also assessed. At 24 h after UVC irradiation 40% of cells derived from young rats were found to be apoptotic, which was twice as much as in splenocytes from old rats. Apoptosis in cells from old rats did not give typical symptoms like a "DNA ladder" and Bcl-2 protein downregulation, in contrast to splenocytes from young rats. No AP-1 transcription factor activity was found in UVC-irradiated splenocytes from old animals and only a trace activity in splenocytes from young animals. This indicates that, UVC-induced apoptosis in rat splenocytes is practically AP-1 independent and that cells from old rats are less sensitive to UVC irradiation than splenocytes from young rats.

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