Identification of a PARP Inhibitor Sensitivity Signature in Breast Cancer Using a Novel Transcription Factor Activity Array

2012 ◽  
Author(s):  
Lonnie D. Shea
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Parp Inhibitor ◽  
Transcription Factor Activity ◽  
Factor Activity

scholarly journals The orphan nuclear receptor estrogen-related receptor beta (ERRβ) in triple-negative breast cancer

2019 ◽  
Author(s):  
Aileen I. Fernandez ◽  
Xue Geng ◽  
Krysta Chaldekas ◽  
Brent Harris ◽  
Anju Duttargi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Mrna Expression ◽  
Cell Lines ◽  
Copy Number ◽  
Orphan Nuclear Receptor ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Tnbc Cell ◽  
Estrogen Related Receptor

ABSTRACTPurposeTriple negative breast cancer (TNBC)/ basal-like breast cancer (BLBC) is a highly aggressive form of breast cancer prevalent in African-American (AA) women. We previously reported that a small molecule agonist ligand for the orphan nuclear receptor estrogen-related receptor beta (ERRβ or ESRRB) has growth inhibitory and anti-mitotic activity in TNBC cell lines. In this study, we evaluate the association of ESRRB mRNA, copy number levels, and protein expression with demographic, clinicopathological, and gene expression features in breast tumor clinical specimens.MethodsESRRB mRNA level expression and clinical associations were analyzed using RNAseq data. Array-based comparative genomic hybridization determined ESRRB copy number in AA and Caucasian women. Transcription factor activity was measured using promoter-reporter luciferase assays in TNBC cell lines. Semi-automatic quantification of immunohistochemistry measured ERRβ protein expression on a 150-patient tissue microarray series.ResultsESRRB mRNA expression is significantly lower in TNBC/BLBC vs. other breast cancer subtypes. There is no evidence of ESRRB copy number loss. ESRRB mRNA expression is correlated with the expression of genes associated with neuroactive ligand-receptor interaction, metabolic pathways, and deafness. These genes contain G/C-rich transcription factor binding motifs. The ESRRB message is alternatively spliced into three isoforms, which we show have different transcription factor activity in basal-like vs. other TNBC cell lines. We further show that the ERRβ2 and ERRβsf isoforms are broadly expressed in breast tumors at the protein level.ConclusionsDecreased ESRRB mRNA expression, and distinct patterns of ERRβ isoform subcellular localization and transcription factor activity are key features in TNBC/BLBC.

scholarly journals Identification of Post-Transcriptional Modulators of Breast Cancer Transcription Factor Activity Using MINDy

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168770 ◽  
Author(s):  
Thomas M. Campbell ◽  
Mauro A. A. Castro ◽  
Bruce A. J. Ponder ◽  
Kerstin B. Meyer
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Transcriptional Modulators

Modulation of the Escherichia coli σ E (RpoE) heat‐shock transcription‐factor activity by the RseA, RseB and RseC proteins

1997 ◽  
Vol 24 (2) ◽  
pp. 355-371 ◽  
Author(s):  
Dominique Missiakas ◽  
Matthias P. Mayer ◽  
Marc Lemaire ◽  
Costa Georgopoulos ◽  
Satish Raina
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Heat Shock ◽  
Heat Shock Transcription Factor ◽  
Transcription Factor Activity ◽  
Factor Activity
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