Expression of Multisubunit Proteins in Leishmania tarentolae

2011 ◽  
pp. 317-325 ◽  
Author(s):  
Marisa Sugino ◽  
Tomoaki Niimi
Keyword(s):  
Leishmania Tarentolae

Structural characterization of naphthalene sulfonamides and a sulfonate ester and their in vitro efficacy against Leishmania tarentolae promastigotes

2021 ◽  
Vol 45 (10) ◽  
pp. 4791-4801
Author(s):  
Edward W. Li ◽  
Jade Katinas ◽  
Marjorie A. Jones ◽  
Christopher G. Hamaker
Keyword(s):  
Biological Activity ◽  
Structural Characterization ◽  
Leishmania Tarentolae ◽  
Sulfonate Ester

Structural and biological activity analyses of two naphthalene sulfonamides and a naphthalene sulfonate ester.

scholarly journals Leishmania tarentolae novel responses to Bi3+-doped strontium aluminum oxyfluorides

Heliyon ◽  
2021 ◽  
pp. e07896
Author(s):  
C. Fiore Apuzzo ◽  
Eirin C. Sullivan ◽  
David C. Platt ◽  
Ian Seger-Held ◽  
Marjorie A. Jones
Keyword(s):  
Leishmania Tarentolae

An intergenic G-rich region in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited cryptogene encoding ribosomal protein S12

1992 ◽  
Vol 12 (1) ◽  
pp. 56-67
Author(s):  
D A Maslov ◽  
N R Sturm ◽  
B M Niner ◽  
E S Gruszynski ◽  
M Peris ◽  
...  
Keyword(s):  
Amino Acid ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Sequence Similarity ◽  
Rich Region ◽  
Leishmania Tarentolae ◽  
Significant Sequence Similarity ◽  
The Family ◽  
Intergenic Regions ◽  
Ribosomal Protein S12

Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added at 49 sites and 32 uridines are deleted at 13 sites, creating a translated 85-amino-acid polypeptide. Similar polypeptides are probably encoded by pan-edited G6 transcripts in two other species. The G6 polypeptide has significant sequence similarity to the family of S12 ribosomal proteins. A minicircle-encoded gRNA overlaps 12 editing sites in G6 mRNA, and chimeric gRNA/mRNA molecules were shown to exist, in agreement with the transesterification model for editing.

scholarly journals Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19

2021 ◽  
Vol 12 ◽  
Author(s):  
Ilaria Varotto-Boccazzi ◽  
Alessandro Manenti ◽  
Francesca Dapporto ◽  
Louise J. Gourlay ◽  
Beatrice Bisaglia ◽  
...  
Keyword(s):  
Rapid Development ◽  
Antibody Detection ◽  
Molecular Diagnostic ◽  
Serological Tests ◽  
Leishmania Tarentolae ◽  
Cell Factories ◽  
Effective System ◽  
Human Sera ◽  
Antiviral Antibody ◽  
Viral Diagnosis

To detect and prevent emerging epidemics, discovery platforms are urgently needed, for the rapid development of diagnostic assays. Molecular diagnostic tests for COVID-19 were developed shortly after the isolation of SARS-CoV-2. However, serological tests based on antiviral antibody detection, revealing previous exposure to the virus, required longer testing phases, due to the need to obtain correctly folded and glycosylated antigens. The delay between the identification of a new virus and the development of reliable serodiagnostic tools limits our readiness to tackle future epidemics. We suggest that the protozoan Leishmania tarentolae can be used as an easy-to-handle microfactory for the rapid production of viral antigens to face emerging epidemics. We engineered L. tarentolae to express the SARS-CoV-2 receptor-binding domain (RBD) and we recorded the ability of the purified RBD antigen to detect SARS-CoV-2 infection in human sera, with a sensitivity and reproducibility comparable to that of a reference antigen produced in human cells. This is the first application of an antigen produced in L. tarentolae for the serodiagnosis of a Coronaviridae infection. On the basis of our results, we propose L. tarentolae as an effective system for viral antigen production, even in countries that lack high-technology cell factories.

scholarly journals Genome sequencing of the lizard parasite Leishmania tarentolae reveals loss of genes associated to the intracellular stage of human pathogenic species

2011 ◽  
Vol 40 (3) ◽  
pp. 1131-1147 ◽  
Author(s):  
Frédéric Raymond ◽  
Sébastien Boisvert ◽  
Gaétan Roy ◽  
Jean-François Ritt ◽  
Danielle Légaré ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Pathogenic Species ◽  
Leishmania Tarentolae ◽  
Parasite Leishmania ◽  
Intracellular Stage

scholarly journals Disruption of RNA editing in Leishmania tarentolae by the loss of minicircle-encoded guide RNA genes.

1994 ◽  
Vol 13 (23) ◽  
pp. 5689-5700 ◽  
Author(s):  
O.H. Thiemann ◽  
D.A. Maslov ◽  
L. Simpson
Keyword(s):  
Rna Editing ◽  
Leishmania Tarentolae ◽  
Guide Rna ◽  
Rna Genes
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