Defining Hierarchies of Unrestricted Somatic Stem Cells and Mesenchymal Stem Cells in Cord Blood

2011 ◽  
pp. 71-85
Author(s):  
Gesine Kögler
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Somatic Stem Cells ◽  
Unrestricted Somatic Stem Cells

A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue

2011 ◽  
Vol 33 (6) ◽  
pp. 1257-1264 ◽  
Author(s):  
Abbas Shafiee ◽  
Ehsan Seyedjafari ◽  
Masoud Soleimani ◽  
Naser Ahmadbeigi ◽  
Peyman Dinarvand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Somatic Stem Cells ◽  
Unrestricted Somatic Stem Cells

Potentials of Differentiated Human Cord Blood-Derived Unrestricted Somatic Stem Cells in Treatment of Liver Cirrhosis

2019 ◽  
Vol 17 (2) ◽  
pp. 251-258
Author(s):  
Hanan El Baz ◽  
◽  
Zeinab Demerdash ◽  
Manal Kamel ◽  
Shimaa Atta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Liver Cirrhosis ◽  
Cord Blood ◽  
Somatic Stem Cells ◽  
Human Cord Blood ◽  
Unrestricted Somatic Stem Cells

In vitro Differentiation of Human Cord Blood-Derived Unrestricted Somatic Stem Cells into Hepatocyte-Like Cells on Poly(ε-Caprolactone) Nanofiber Scaffolds

2009 ◽  
Vol 190 (3) ◽  
pp. 135-149 ◽  
Author(s):  
Seyed Mahmoud Hashemi ◽  
Masoud Soleimani ◽  
Seyed Shahrooz Zargarian ◽  
Vahid Haddadi-Asl ◽  
Naser Ahmadbeigi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
In Vitro Differentiation ◽  
Somatic Stem Cells ◽  
Human Cord Blood ◽  
Nanofiber Scaffolds ◽  
Unrestricted Somatic Stem Cells

scholarly journals Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Foued Ghanjati ◽  
Simeon Santourlidis
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Epigenetic Mechanism ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Somatic Stem Cells ◽  
Epigenetic State ◽  
Unrestricted Somatic Stem Cells

The epigenetic mechanism of DNA methylation is of central importance for cellular differentiation processes. Unrestricted somatic stem cells (USSCs) from human umbilical cord blood, which have a broad differentiation spectrum, reside in an uncommitted epigenetic state with partial methylation of the regulatory region of the gene coding for the pluripotency master regulator OCT4. Thus we hypothesized that further opening of this “poised” epigenetic state could broaden the differentiation potential of USSCs. Here we document that USSCs drastically change their phenotype after treatment by a new elaborated cultivation protocol which utilizes the DNA hypomethylating compound 5′-aza-2-deoxycytidine (5-Aza-CdR) and the histone deacetylase inhibitor trichostatin A (TSA). This treatment leads to a new stable, spheroid-forming cell type which we have named SpheUSSC. These cells can be stably propagated over at least 150 cell divisions, express OCT4, retain the potential to undergo osteogenic differentiation, and have additionally acquired the ability to uniformly differentiate into adipocytes, unlike the source USSC population. Here we describe our treatment protocol and provide evidence that it induces a dedifferentiation step and concomitantly the acquisition of an extended differentiation capability of the new SpheUSSC type.

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