Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

2011 ◽  
pp. 19-25 ◽  
Author(s):  
Bertrand Jauffred ◽  
Yohanns Bellaiche
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Live Imaging ◽  
Precursor Cell ◽  
Sensory Organ ◽  
Sensory Organ Precursor

Role of Notch and achaete-scute complex in the expression of Enhancer of split bHLH proteins

Development ◽  
1995 ◽  
Vol 121 (11) ◽  
pp. 3745-3752 ◽  
Author(s):  
V. Jennings ◽  
J. de Celis ◽  
C. Delidakis ◽  
A. Preiss ◽  
S. Bray
Keyword(s):  
Cell Fate ◽  
Notch Pathway ◽  
Interaction Mechanism ◽  
Cell Interaction ◽  
Precursor Cell ◽  
Notch Receptor ◽  
Sensory Organ ◽  
Cell Fate Decisions ◽  
Sensory Organ Precursor ◽  
Enhancer Of Split

The proteins encoded by Notch and the Enhancer of split complex are components of a cell-cell interaction mechanism which is important in many cell fate decisions throughout development. One such decision is the formation of the sensory organ precursor cell during the development of the peripheral nervous system in Drosophila. Cells acquire the potential to be neural through the expression of the proneural genes, and the Notch pathway is required to limit neural fate to a single cell from a proneural cluster. However, despite extensive analysis, the precise pathways linking the proneural with Notch and Enhancer of split gene functions remain obscure. For example, it has been suggested that achaete-scute complex proteins directly activate Enhancer of split genes leaving the action of Notch in the pathway unclear. Using monoclonal antibodies that recognise products of the Enhancer of split complex, we show that these proteins accumulate in the cells surrounding the developing sensory organ precursor cell and that their expression is dependent on the activity of Notch and does not directly correlate with expression of Achaete. We further clarify the pathway by showing that ubiquitous expression of an activated Notch receptor leads to widespread accumulation of Enhancer of split proteins even in the absence of achaete-scute complex proteins. Thus Enhancer of split protein expression in response to Notch activity does not require achaete-scute complex proteins.

Two types of asymmetric divisions in the Drosophila sensory organ precursor cell lineage

2000 ◽  
Vol 3 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Fabrice Roegiers ◽  
Susan Younger-Shepherd ◽  
Lily Yeh Jan ◽  
Yuh Nung Jan
Keyword(s):  
Cell Lineage ◽  
Precursor Cell ◽  
Sensory Organ ◽  
Asymmetric Divisions ◽  
Sensory Organ Precursor

scholarly journals Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding γ-secretase

2013 ◽  
Vol 201 (3) ◽  
pp. 439-448 ◽  
Author(s):  
Alok Upadhyay ◽  
Vasundhara Kandachar ◽  
Diana Zitserman ◽  
Xin Tong ◽  
Fabrice Roegiers
Keyword(s):  
Notch Signaling ◽  
Cell Fate ◽  
Daughter Cell ◽  
Asymmetric Cell Division ◽  
Notch Receptor ◽  
Receptor Internalization ◽  
Sensory Organ ◽  
Cell Fates ◽  
Cellular Context ◽  
Sensory Organ Precursor

In Drosophila peripheral neurogenesis, Notch controls cell fates in sensory organ precursor (SOP) cells. SOPs undergo asymmetric cell division by segregating Numb, which inhibits Notch signaling, into the pIIb daughter cell after cytokinesis. In contrast, in the pIIa daughter cell, Notch is activated and requires Sanpodo, but its mechanism of action has not been elucidated. As Sanpodo is present in both pIIa and pIIb cells, a second role for Sanpodo in regulating Notch signaling in the low-Notch pIIb cell has been proposed. Here we demonstrate that Sanpodo regulates Notch signaling levels in both pIIa and pIIb cells via distinct mechanisms. The interaction of Sanpodo with Presenilin, a component of the γ-secretase complex, was required for Notch activation and pIIa cell fate. In contrast, Sanpodo suppresses Notch signaling in the pIIb cell by driving Notch receptor internalization. Together, these results demonstrate that a single protein can regulate Notch signaling through distinct mechanisms to either promote or suppress signaling depending on the local cellular context.

scholarly journals Drosophila E-Cadherin Regulates the Orientation of Asymmetric Cell Division in the Sensory Organ Lineage

2002 ◽  
Vol 12 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Roland Le Borgne ◽  
Yohanns Bellaı̈che ◽  
François Schweisguth
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Sensory Organ ◽  
E Cadherin
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