Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos

2012 ◽  
pp. 91-111 ◽  
Author(s):  
Sally A. Moody
Keyword(s):  
Gene Expression ◽  
Xenopus Embryos

scholarly journals Inhibition of Xhox1A gene expression in Xenopus embryos by antisense RNA produced from an expression vector read by RNA polymerase III

1995 ◽  
Vol 52 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Anthony Nichols ◽  
Elisabeth Rungger-Brändle ◽  
Lisbeth Muster ◽  
Duri Rungger
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Antisense Rna ◽  
Expression Vector ◽  
Rna Polymerase Iii ◽  
Xenopus Embryos ◽  
Polymerase Iii

scholarly journals Inducible gene expression in transgenic Xenopus embryos

2000 ◽  
Vol 10 (14) ◽  
pp. 849-852 ◽  
Author(s):  
Grant N. Wheeler ◽  
Fiona S. Hamilton ◽  
Stefan Hoppler
Keyword(s):  
Gene Expression ◽  
Inducible Gene Expression ◽  
Xenopus Embryos ◽  
Inducible Gene

An expression vector inhibits gene expression in Xenopus embryos by antisense RNA

1992 ◽  
Vol 201 (6) ◽  
pp. 340-345 ◽  
Author(s):  
Michael Schmid ◽  
Herbert Steinbeisser ◽  
Hans-Henning Epperlein ◽  
Michael F. Trendelenburg ◽  
Hans J. Lipps
Keyword(s):  
Gene Expression ◽  
Antisense Rna ◽  
Expression Vector ◽  
Xenopus Embryos

Regulation of XFGF8 gene expression through SRY (sex-determining region Y)-box 2 in developing Xenopus embryos

2012 ◽  
Vol 24 (6) ◽  
pp. 769
Author(s):  
Yong Hwan Kim ◽  
Jee Yoon Shin ◽  
Wonho Na ◽  
Jungho Kim ◽  
Bong-Gun Ju ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Activation ◽  
Upstream Region ◽  
Binding Motif ◽  
Vertebrate Development ◽  
Cis Element ◽  
Xenopus Embryos ◽  
Lens Placode

Fibroblast growth factors (FGFs) function as mitogens and morphogens during vertebrate development. In the present study, to characterise the regulatory mechanism of FGF8 gene expression in developing Xenopus embryos the upstream region of the Xenopus FGF8 (XFGF8) gene was isolated. The upstream region of the XFGF8 gene contains two putative binding sites for the SRY (sex-determining region Y)-box 2 (SOX2) transcription factor. A reporter assay with serially deleted constructs revealed that the putative SOX2-binding motif may be a critical cis-element for XFGF8 gene activation in developing Xenopus embryos. Furthermore, Xenopus SOX2 (XSOX2) physically interacted with the SOX2-binding motif within the upstream region of the XFGF8 gene in vitro and in vivo. Depletion of endogenous XSOX2 resulted in loss of XFGF8 gene expression in midbrain–hindbrain junction, auditory placode, lens placode and forebrain in developing Xenopus embryos. Collectively, our results suggest that XSOX2 directly upregulates XFGF8 gene expression in the early embryonic development of Xenopus.

Drosophila short gastrulation induces an ectopic axis in Xenopus: evidence for conserved mechanisms of dorsal-ventral patterning

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4319-4328 ◽  
Author(s):  
J. Schmidt ◽  
V. Francois ◽  
E. Bier ◽  
D. Kimelman
Keyword(s):  
Gene Expression ◽  
Ultraviolet Irradiation ◽  
Ventral Side ◽  
Gastrula Stage ◽  
Spemann Organizer ◽  
Xenopus Embryos ◽  
Bmp Receptors ◽  
Secondary Axis ◽  
Drosophila Embryos

The Spemann organizer has long been recognized as a major source of patterning signals during the gastrula stage of amphibian embryogenesis. More recent evidence has suggested that the ventral side of the embryo also plays an important role in dorsal-ventral patterning during gastrulation through the action of signaling factors such as BMP-4. Bmp-4 is closely related to the Drosophila decapentaplegic (dpp) gene, and like Bmp-4, dpp is excluded from the neurogenic region. Recently we showed that Bmp-4 functions in an analogous role to that of dpp in Drosophila, suggesting that the mechanism of dorsal-ventral patterning in Xenopus and Drosophila embryos may be conserved. To further test this hypothesis, RNA of the Drosophila short gastrulation (sog) gene was injected into Xenopus embryos, since sog has been shown genetically to be an antagonist of dpp function. Overexpression of sog RNA in Xenopus dorsalizes the embryo by expanding neurogenic and dorsal paraxial tissue. When ectopically expressed on the ventral side of the embryo, sog induces a partial secondary axis. In addition, sog partially rescues embryos ventralized by ultraviolet irradiation. Since sog induces many similar changes in gene expression to that caused by truncated BMP receptors, we suggest that sog functions in part by opposing BMP-4 signaling. The recent identification of a possible Xenopus sog homolog, chordin, in conjunction with these results supports the hypothesis that dorsal-ventral patterning mechanisms are conserved between these two species.

GENE EXPRESSION IN XENOPUS EMBRYOS AFTER METHYLMERCURY EXPOSURE: A SEARCH FOR MOLECULAR BIOMARKERS

2002 ◽  
Vol 21 (12) ◽  
pp. 2731
Author(s):  
Claudio Monetti ◽  
Davide Vigetti ◽  
Mariangela Prati ◽  
Enrico Sabbioni ◽  
Giovanni Bernardini ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Biomarkers ◽  
Xenopus Embryos ◽  
Methylmercury Exposure
Sign in / Sign up

Export Citation Format

Share Document