Nonradioactive In Situ Hybridization on Skeletal Tissue Sections

2014 ◽  
pp. 203-215 ◽  
Author(s):  
Yulia Shwartz ◽  
Elazar Zelzer
Keyword(s):  
In Situ Hybridization ◽  
Skeletal Tissue ◽  
Tissue Sections ◽  
Nonradioactive In Situ Hybridization

Nonradioactive in Situ Hybridization to Xenopus Tissue Sections

Methods ◽  
2001 ◽  
Vol 23 (4) ◽  
pp. 303-312 ◽  
Author(s):  
Karen Butler ◽  
Aaron M. Zorn ◽  
J.B. Gurdon
Keyword(s):  
In Situ Hybridization ◽  
Tissue Sections ◽  
Nonradioactive In Situ Hybridization

scholarly journals Detection of Porcine Reproductive and Respiratory Syndrome Virus in Cell Cultures and Formalin-Fixed Tissues by in Situ Hybridization using a Digoxigenin-Labeled Probe

1996 ◽  
Vol 8 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Renée Larochelle ◽  
Helmi Mardassi ◽  
Serge Dea ◽  
Ronald Magar
Keyword(s):  
In Situ Hybridization ◽  
Cdna Probe ◽  
Respiratory Syndrome Virus ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Labeled Probe ◽  
Nonradioactive In Situ Hybridization ◽  
Formalin Fixed

A nonradioactive in situ hybridization method is described for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) in cell cultures and in formalin-fixed paraffin-embedded tissue sections originating from experimentally infected pigs and from 1 field case. A 174 bp cDNA probe targeting the viral RNA encoding the nucleocapsid protein of a Canadian PRRSV isolate was generated by polymerase chain reaction. The cDNA probe was labeled by random priming with digoxigenin-dUTP using a commercially available kit. The ability of the digoxigenin-labeled probe to specifically detect PRRSV RNA was tested on cultured cells infected with 6 Canadian PRRSV isolates, a US PRRSV isolate and the European Lelystad isolate. The probe detected all Canadian PRRSV isolates tested as well as the US PRRSV isolate but did not detect the Lelystad isolate. In addition, when tested on formalin-fixed paraffin-embedded tissue sections from pigs experimentally infected with several Canadian isolates and from a field case, a strong signal without background staining was obtained. Our results indicate that nonradioactive in situ hybridization could represent a useful tool for the detection of PRRSV in routinely fixed and processed tissues. In situ hybridization could also be used to differentiate infection by North American and European Lelystad-like PRRSV isolates.

Application of in situ hybridization with a novel phenytoin-labeled probe to conventional formalin-fixed, paraffin-embedded tissue sections

1995 ◽  
Vol 52 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Yoshito Eizuru ◽  
Yoichi Minamishima ◽  
Tadashi Matsumoto ◽  
Toshinari Hamakado ◽  
Mikio Mizukoshi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Labeled Probe ◽  
Formalin Fixed

Nonradioactive in Situ Hybridization with Digoxigenin Labeled DNA Probes

1992 ◽  
Vol 67 (2) ◽  
pp. 59-67 ◽  
Author(s):  
N. Arnold ◽  
R. Seibl ◽  
C. Kessler ◽  
J. Wienberg
Keyword(s):  
In Situ Hybridization ◽  
Dna Probes ◽  
Nonradioactive In Situ Hybridization
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