Utilization of Endophytic Bacteria Isolated from Legume Root Nodules for Plant Growth Promotion

One of the ways to increase the competitive survivability of rhizobial biofertilizers and thus achieve better plant growth under such conditions is by modifying the rhizospheric environment or community by addition of nonrhizobial nodule-associated bacteria (NAB) that cause better nodulation and plant growth when coinoculated with rhizobia. A study was performed to investigate the most commonly associated nodule-associated bacteria and the rhizospheric microorganisms associated with theFenugreek(Trigonella foenum-graecum) plant. Isolation of nonrhizobial isolates from root nodules ofFenugreekwas carried out along with the rhizospheric isolates. About 64.7% isolates obtained fromFenugreeknodules were gram-negative coccobacilli, 29.41% were gram-positive bacilli, and all rhizospheric isolates except one were gram-positive bacilli. All the isolates were characterized for their plant growth promoting (PGP) activities. Two of the NAB isolates M2N2c and B1N2b (Exiguobacterium sp.) showed maximum positive PGP features. Those NAB isolates when coinoculated with rhizobial strain—S. meliloti, showed plant growth promotion with respect to increase in plant’s root and shoot length, chlorophyll content, nodulation efficiency, and nodule dry weight.

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Isolation of phosphate solubilizing endophytic bacteria from Phyllanthus amarus Schum & Thonn: Evaluation of plant growth promotion and antioxidant activity under salt stress

Journal of Applied Research on Medicinal and Aromatic Plants ◽

10.1016/j.jarmap.2016.02.003 ◽

2016 ◽

Vol 3 (2) ◽

pp. 71-77 ◽

Cited By ~ 17

Author(s):

Manoharan Melvin Joe ◽

Shalini Devaraj ◽

Abitha Benson ◽

Tongmin Sa

Keyword(s):

Antioxidant Activity ◽

Salt Stress ◽

Plant Growth ◽

Plant Growth Promotion ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Phyllanthus Amarus ◽

Phosphate Solubilizing

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Interactions between putatively endophytic bacteria and tall fescue (Festuca arundinacea): plant growth promotion and colonization in host and non-host cultivars

Plant and Soil ◽

10.1007/s11104-019-04359-5 ◽

2019 ◽

Vol 451 (1-2) ◽

pp. 207-220

Author(s):

Patricia Vaz Jauri ◽

Cecilia Taulé ◽

Maria Cecilia de los Santos ◽

Belén Fernandez ◽

Andrés Di Paolo ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Endophytic Bacteria ◽

Growth Promotion

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Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

Microorganisms ◽

10.3390/microorganisms8040499 ◽

2020 ◽

Vol 8 (4) ◽

pp. 499 ◽

Cited By ~ 2

Author(s):

Ricardo Soares ◽

Jesús Trejo ◽

Maria J. Lorite ◽

Etelvina Figueira ◽

Juan Sanjuán ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Root Nodules ◽

Nifh Gene ◽

Bacterial Strains ◽

Lytic Enzymes ◽

Taxonomic Assignment ◽

Pgp Traits ◽

Associated Bacteria

Lotus spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few Lotus species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild Lotus parviflorus plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the Bradyrhizobium genus, which is closely related to B. canariense and other Bradyrhizobium sp. strains isolated from genistoid legumes and Ornithopus spp. Symbiotic nodC and nifH gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- (Rhizobium/Agrobacterium), beta- (Massilia) and gamma-proteobacteria (Pseudomonas, Lysobacter, Luteibacter, Stenotrophomonas and Rahnella), as well as some bacteroidetes from genera Sphingobacterium and Mucilaginibacter. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these L. parviflorus nodule-associated bacteria.

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Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa)

Acta Amazonica ◽

10.1590/1809-4392201804831 ◽

2019 ◽

Vol 49 (4) ◽

pp. 257-267

Author(s):

Patrícia Bombonati CHALITA ◽

Eliane do Nascimento Cunha FARIAS ◽

Ismaele Breckenfeld da COSTA ◽

Brenda Ferreira SOUSA ◽

Marco Antônio Oliveira dos SANTOS ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Nifh Gene ◽

Aluminum Phosphate ◽

Brazil Nut ◽

Indole Compounds ◽

Plant Growth Promoting ◽

Growth Promoting

ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene. Plant growth-promoting characteristics were evaluated by the presence of the nifH gene, aluminum phosphate solubilization and the production of indole compounds. The isolates were affiliated with 18 genera belonging to 5 different classes (α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacilli and Actinobacteria). The genus Bacillus was predominant in the forest and monoculture areas. Fourteen isolates presented the nifH gene. Most of the bacteria were able to solubilize aluminum phosphate and synthetize indole compounds. The results indicated high diversity of endophytic bacteria present among the roots of Brazil nut trees, mainly in the agroforestry area, which could be related to soil attributes. Among the 90 isolates, the 22 that presented the best results regarding plant growth promotion traits were good candidates for testing in seedling production of Brazil nut trees.

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