Molecular Ecology of the Southern Gray Brocket Deer (Mazama gouazoubira Fischer, 1814)

The Brown brocket deer (Mazama gouazoubira) is the most common free-living and captive deer in South America, especially in Brazil, and has great ecological and scientific significance. However, data on hematological and biochemical parameters in brown brocket deer are scarce. The goal of this study was to establish reference ranges for hematological and biochemical parameters of Mazama gouazoubira, comparing differences during the seasons of the year and between sex. Blood samples from ten adult healthy brown brocket deer (6 female and 4 male) were collected during daytime, monthly, during 12 months. The animals were maintained in individual stable, protected from noise and fed ad libitum with commercial ration and green fodder. For blood collection, animals were submitted to physical restrain for no longer than 2 minutes. The following parameters were determined: red blood cell count (RBC), haemoglobin concentration, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), white blood cell count (WBC), platelet count, enzyme activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) and serum levels of alkaline phosphatase (ALP), creatine kinase (CK), total protein (TP), albumin, cholesterol, total calcium, ionic calcium, sodium, potassium, magnesium, triglycerides, creatinine and urea. Values were compared according to season and sex. RBC count, WBC count and MCV suggested seasonal influence. Haemoglobin concentration, PCV and MCV were influenced by sex. Serum concentration of total calcium, ionic calcium, sodium, potassium and magnesium were influenced by season. Serum magnesium was also influenced by sex. The blood parameters herein reported may be useful as reference values for diagnostic and prognostic purposes in captive brown-brocket deer.

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Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Zygote ◽

10.1017/s0967199419000741 ◽

2019 ◽

Vol 28 (2) ◽

pp. 170-173

Author(s):

Thaís T.S. Souza ◽

Maria J.B. Bezerra ◽

Maurício F. van Tilburg ◽

Celso S. Nagano ◽

Luciana D. Rola ◽

...

Keyword(s):

Mass Spectrometry ◽

Follicular Fluid ◽

Protein Profile ◽

Mass Spectrometry Data ◽

Mass Spectrometry Analysis ◽

Spectrometry Analysis ◽

Significant Difference ◽

Mazama Gouazoubira ◽

Ovarian Follicular Fluid ◽

Brocket Deer

SummaryThe aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P > 0.05) was observed between small/medium and large follicles: 49.2 ± 22.8 and 56.7 ± 27.4 μg/μl, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P > 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.

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Histomorphometric evaluation of the neotropical brown brocket deer Mazama gouazoubira testis, with an emphasis on cell population indexes of spermatogenic yield

Animal Reproduction Science ◽

10.1016/j.anireprosci.2011.07.016 ◽

2011 ◽

Vol 127 (3-4) ◽

pp. 202-212 ◽

Cited By ~ 24

Author(s):

Kyvia Lugate Cardoso Costa ◽

Sérgio Luis Pinto da Matta ◽

Marcos de Lucca Moreira Gomes ◽

Tarcízio Antônio Rêgo de Paula ◽

Karine Moura de Freitas ◽

...

Keyword(s):

Cell Population ◽

Mazama Gouazoubira ◽

Brocket Deer

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Managed forest as habitat for gray brocket deer (Mazama gouazoubira) in agricultural landscapes of southeastern Brazil

Journal of Mammalogy ◽

10.1093/jmammal/gyx099 ◽

2017 ◽

Cited By ~ 1

Author(s):

Thiago Ferreira Rodrigues ◽

Roland Kays ◽

Arielle Parsons ◽

Natalia Fraguas Versiani ◽

Roberta Montanheiro Paolino ◽

...

Keyword(s):

Agricultural Landscapes ◽

Southeastern Brazil ◽

Managed Forest ◽

Mazama Gouazoubira ◽

Brocket Deer

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Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814)

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612014035 ◽

2014 ◽

Vol 23 (2) ◽

pp. 206-215 ◽

Cited By ~ 13

Author(s):

Júlia Angélica Gonçalves da Silveira ◽

Élida Mara Leite Rabelo ◽

Paula Cristina Senra Lima ◽

Bárbara Neves Chaves ◽

Múcio Flávio Barbosa Ribeiro

Keyword(s):

Nested Pcr ◽

Pcr Analysis ◽

Post Mortem ◽

Infectious Agents ◽

Free Living ◽

Molecular Assays ◽

Minas Gerais State ◽

Mazama Gouazoubira ◽

Nested Pcr Assays ◽

Brocket Deer

Tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. The aim of the present study was the post-mortem detection of hemoparasites in free-living Mazama gouazoubira from Minas Gerais state, Brazil. The deer samples consisted of free-living M. gouazoubira (n = 9) individuals that died after capture. Necropsy examinations of the carcasses were performed to search for macroscopic alterations. Organ samples were collected for subsequent imprint slides, and nested PCR assays were performed to detect hemoparasite species. Imprint slide assays from four deer showed erythrocytes infected with Piroplasmida small trophozoites, and A. marginale corpuscles were observed in erythrocytes from two animals. A. marginale and trophozoite co-infections occurred in two deer. A nested PCR analysis of the organs showed that six of the nine samples were positive for Theileria sp., five were positive for A. phagocytophilum and three were positive for A. marginale, with co-infection occurring in four deer. The results of the present study demonstrate that post-mortemdiagnostics using imprint slides and molecular assays are an effective method for detecting hemoparasites in organs.

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A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mazama gouazoubira)

PLoS ONE ◽

10.1371/journal.pone.0198670 ◽

2018 ◽

Vol 13 (6) ◽

pp. e0198670 ◽

Cited By ~ 4

Author(s):

Pedro Enrique Navas-Suárez ◽

Josué Díaz-Delgado ◽

Eliana Reiko Matushima ◽

Cintia Maria Fávero ◽

Angélica Maria Sánchez Sarmiento ◽

...

Keyword(s):

Deer Species ◽

Mazama Gouazoubira ◽

Blastocerus Dichotomus ◽

Brocket Deer ◽

Neotropical Deer

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Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay

Microorganisms ◽

10.3390/microorganisms9102165 ◽

2021 ◽

Vol 9 (10) ◽

pp. 2165

Author(s):

María Laura Félix ◽

Sebastián Muñoz-Leal ◽

Luis Andrés Carvalho ◽

Diego Queirolo ◽

Susana Remesar ◽

...

Keyword(s):

Molecular Detection ◽

Pathogenic Role ◽

Free Living ◽

Epidemiological Factors ◽

The Third ◽

Mazama Gouazoubira ◽

Potential Pathogenicity ◽

Human Ehrlichiosis ◽

Brocket Deer

Human ehrlichiosis are scantily documented in Uruguay. The aim of this study was to investigate the presence of Ehrlichia spp. in Haemaphysalis juxtakochi and in a gray brocket deer (Mazama gouazoubira) from Uruguay. The presence of Ehrlichia DNA was investigated in free-living H. juxtakochi in five localities of southeast and northeast Uruguay, as well as blood, spleen, and ticks retrieved from a M. gouazoubira. Ehrlichia spp. DNA was detected in six out of 99 tick pools from vegetation, in the spleen of M. gouazoubira, and in one out of five pools of ticks feeding on this cervid. Bayesian inference analyses for three loci (16S rRNA, dsb, and groEL) revealed the presence of a new rickettsial organism, named herein as “Candidatus Ehrlichia pampeana”. This new detected Ehrlichia is phylogenetically related to those found in ticks from Asia, as well as Ehrlichia ewingii from USA and Cameroon. Although the potential pathogenicity of “Ca. E. pampeana” for humans is currently unknown, some eco-epidemiological factors may be relevant to its possible pathogenic role, namely: (i) the phylogenetic closeness with the zoonotic agent E. ewingii, (ii) the evidence of H. juxtakochi parasitizing humans, and (iii) the importance of cervids as reservoirs for zoonotic Ehrlichia spp. The molecular detection of “Ca. E. pampeana” represents the third Ehrlichia genotype described in Uruguay.

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