Intestinal Perfusion Models

The flower bud of Daphne genkwa (Genkwa Flos) is a commonly used herbal medicine in Asian countries. Luteolin and apigenin are two recognized active flavonoids in Genkwa Flos. The aim of this study was to investigate the intestinal absorption mechanisms of Genkwa Flos flavonoids using in situ single-pass intestinal perfusion rat model. Using HPLC, we determined its major effective flavonoids luteolin, apigenin, as well as, hydroxygenkwanin and genkwanin in biological samples. The intestinal absorption mechanisms of the total flavonoids in Genkwa Flos (TFG) were investigated using in situ single-pass intestinal perfusion rat model. Comparing the TFG absorption rate in different intestinal segments, data showed that the small intestine absorption was significantly higher than that of the colon ([Formula: see text]). Compared with duodenum and ileum, the jejunum was the best small intestinal site for TFG absorption. The high TFG concentration (61.48[Formula: see text][Formula: see text]g/ml) yielded the highest permeability ([Formula: see text]). Subsequently, three membrane protein inhibitors (verapamil, pantoprazole and probenecid) were used to explore the TFG absorption pathways. Data showed probenecid, a multidrug resistance protein (or MRP) inhibitor, effectively enhanced the TFG absorption ([Formula: see text]). Furthermore, by comparing commonly used natural absorption enhancers on TFG, it was observed that camphor was the most effective. In Situ single-pass intestinal perfusion experiment shows that TFG absorption is much higher in the small intestine than in the colon, and the TFG is absorbed mainly via an active transport pathway with MRP-mediated efflux mechanism. Camphor obviously enhanced the TFG absorption, and this could be an effective TFG formulation preparation method to increase clinical effectiveness after Genkwa Flos administration. Our study elucidated the TFG absorption mechanisms, and provided new information for its formulation preparation.

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Hypertonic saline-dextran improves intestinal perfusion and survival in porcine endotoxin shock

Critical Care Medicine ◽

10.1097/00003246-200008000-00027 ◽

2000 ◽

Vol 28 (8) ◽

pp. 2843-2850 ◽

Cited By ~ 40

Author(s):

Yoshiyuki Oi ◽

Anders Åneman ◽

Mats Svensson ◽

Sara Ewert ◽

Mats Dahlqvist ◽

...

Keyword(s):

Hypertonic Saline ◽

Endotoxin Shock ◽

Intestinal Perfusion

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Antral volume and propulsion-retropulsion in response to upper intestinal nutrients in a canine model

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1993.264.6.g1077 ◽

1993 ◽

Vol 264 (6) ◽

pp. G1077-G1081

Author(s):

D. Kumar ◽

E. L. Ritman ◽

J. R. Malagelada

Keyword(s):

Small Intestine ◽

Oleic Acid ◽

Isotonic Saline ◽

Casein Hydrolysate ◽

Canine Model ◽

Intestinal Perfusion ◽

Control Solution ◽

Liquid Meal ◽

Small Intestinal

We measured antral volumes and propulsion-retropulsion for individual peristaltic cycles using the dynamic spatial reconstructor in three female mongrel dogs. Our aim was to quantify the effect of intestinal perfusion of equicaloric, isotonic, and isomolar nutrients (maltose, 32.5 mg/100 ml; casein hydrolysate, 32.5 mg/100 ml; and oleic acid 15.5 mg/100 ml) at a rate of 10 ml/min in the upper small intestine on total antral volume and the propulsion-retropulsion function of the "antral pump." Isotonic saline was used as a control solution. Isotonic gastrografin (600 ml) was instilled intragastrically as a meal. A 6-channel perfused manometric assembly was used to record antral phasic pressure activity. Antral volume was significantly lower (P < 0.05) in response to fat perfusion compared with other nutrients or isotonic saline. The propulsion-retropulsion ratio was also significantly reduced (P < 0.05) in response to fat perfusion in the upper small intestine. Our study, therefore, indicates that the fraction of a liquid meal delivered to the antrum is determined, at least in part, by the upper small intestinal nutrients and also that the presence of fat in the small intestine alters the propulsion-retropulsion function of the antrum.

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