Tomographic Reconstruction for Single Conjugate Adaptive Optics

Author(s):  
Jenny Niebsch ◽  
Ronny Ramlau
2013 ◽  
Vol 31 (1) ◽  
pp. 101 ◽  
Author(s):  
C. Correia ◽  
K. Jackson ◽  
J.-P. Véran ◽  
D. Andersen ◽  
O. Lardière ◽  
...  

2019 ◽  
Vol 488 (1) ◽  
pp. 395-400
Author(s):  
Alexandre J T S Mello ◽  
Daniel R Pipa

ABSTRACT Turbulence compensation in astronomy using adaptive optics depends on the use of tomographic techniques to work on wide fields of view. However, classic tomographic methods are computer intensive and consume too much memory to store the tomographic matrix. Recently, various methods have been proposed in an attempt to reduce memory requirements, for example using sparse methods. In this work, we propose a new alternative method that avoids matrices altogether, is faster than classical methods for big systems, and have a very small memory requirement to implement.


2018 ◽  
Vol 27 (2) ◽  
pp. 214-226 ◽  
Author(s):  
Carlos González Gutiérrez ◽  
María Luisa Sánchez Rodríguez ◽  
Ramón Ángel Fernández Díaz ◽  
José Luis Calvo Rolle ◽  
Nieves Roqueñí Gutiérrez ◽  
...  

2020 ◽  
Vol 494 (2) ◽  
pp. 2773-2784 ◽  
Author(s):  
O J D Farley ◽  
J Osborn ◽  
T Morris ◽  
T Fusco ◽  
B Neichel ◽  
...  

ABSTRACT The performance of tomographic adaptive optics (AO) systems is intrinsically linked to the vertical profile of optical turbulence. First, a sufficient number of discrete turbulent layers must be reconstructed to model the true continuous turbulence profile. Secondly over the course of an observation, the profile as seen by the telescope changes and the tomographic reconstructor must be updated. These changes can be due to the unpredictable evolution of turbulent layers on meteorological time-scales as short as minutes. Here, we investigate the effect of changing atmospheric conditions on the quality of tomographic reconstruction by coupling fast analytical AO simulation to a large data base of 10 691 high-resolution turbulence profiles measured over two years by the Stereo-SCIDAR instrument at ESO Paranal, Chile. This work represents the first investigation of these effects with a large, statistically significant sample of turbulence profiles. The statistical nature of the study allows us to assess not only the degradation and variability in tomographic error with a set of system parameters (e.g. number of layers and temporal update period), but also the required parameters to meet some error threshold. In the most challenging conditions where the profile is rapidly changing, these parameters must be far more tightly constrained in order to meet this threshold. By providing estimates of these constraints for a wide range of system geometries as well as the impact of different temporal optimization strategies we may assist the designers of tomographic AO for the extremely large telescope to dimension their systems.


Author(s):  
Neil Rowlands ◽  
Jeff Price ◽  
Michael Kersker ◽  
Seichi Suzuki ◽  
Steve Young ◽  
...  

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).


Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.


Author(s):  
C.L. Woodcock

Despite the potential of the technique, electron tomography has yet to be widely used by biologists. This is in part related to the rather daunting list of equipment and expertise that are required. Thanks to continuing advances in theory and instrumentation, tomography is now more feasible for the non-specialist. One barrier that has essentially disappeared is the expense of computational resources. In view of this progress, it is time to give more attention to practical issues that need to be considered when embarking on a tomographic project. The following recommendations and comments are derived from experience gained during two long-term collaborative projects.Tomographic reconstruction results in a three dimensional description of an individual EM specimen, most commonly a section, and is therefore applicable to problems in which ultrastructural details within the thickness of the specimen are obscured in single micrographs. Information that can be recovered using tomography includes the 3D shape of particles, and the arrangement and dispostion of overlapping fibrous and membranous structures.


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