Serological Methods for Detection of Specific Antibodies to the Duck Hepatitis Virus Type I and Their Assessment

2021 ◽  
pp. 912-917
Author(s):  
Nina Nikitina ◽  
Galina Samuseva ◽  
Konstantin Dmitriev ◽  
Larisa Yavdoshak ◽  
Alexandr Dubovoy
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Type I ◽  
Specific Antibodies ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

scholarly journals Interferonogenic activity of the strain BH-3 duck hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A)

2021 ◽  
Vol 66 (2) ◽  
pp. 162-166
Author(s):  
N. V. Nikitina ◽  
I. K. Leonov ◽  
L. I. Yavdoshak
Keyword(s):  
Cell Culture ◽  
Virus Type ◽  
Hepatitis Virus ◽  
Effective Means ◽  
Culture Method ◽  
Etiologic Agent ◽  
Research Centre ◽  
Type I ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Introduction. Duck viral hepatitis type I (DVH-I) is a poorly studied contagious disease caused by RNA-containing duck (Anatinae) hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A). This infection is widespread in many countries, including Russia, and causes significant damage to industrial duck breeding. The study of interferonogenic activity of its etiologic agent strains is of great importance in solving the problem of developing effective means to control the disease.Material and methods. Strain BH-3 of duck hepatitis virus type I isolated from the liver of sick ducklings was used in the study. The strain was adapted to developing 10–12 day old duck embryos, to the cell culture of chicken and duck fibroblasts and deposited in the State Collection of Viruses of the D.I. Ivanovsky Institute of Virology of FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of Russia. Experiments were performed using the standard tissue culture method.Results and discussion. Data on the ability of the viral strain BH-3 to induce interferon (IFN) and its sensitivity to the action of exogenous interferon in the culture of duck fibroblasts are presented. It has been shown that the interferonogenic activity of this strain of the hepatitis virus is in direct proportion to the multiplicity of infection. The maximum induction of IFN (1 : 256 CEPD50) was observed at a dose of 1.0 TCD50/cell in 72–96 hrs after inoculation of the cell culture. Exogenous IFN at a dose of 1 : 128 completely suppressed the cytopathic effect and death of duck embryos infected with hepatitis virus at a dose of 100 TCD50/cell.Conclusion. The data obtained allow us to state that the vaccine strain BH-3 of duck hepatitis virus type I has a pronounced interferonogenic activity and sensitivity to the action of exogenous IFN. This may have implications for the development of effective therapeutic agents against DVH-I.

scholarly journals Identification of chicken enterovirus-like viruses, duck hepatitis virus type 2 and duck hepatitis virus type 3 as astroviruses

2009 ◽  
Vol 38 (1) ◽  
pp. 21-29 ◽  
Author(s):  
D. Todd ◽  
V. J. Smyth ◽  
N. W. Ball ◽  
B. M. Donnelly ◽  
M. Wylie ◽  
...  
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis ◽  
Type 3

scholarly journals Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae

2006 ◽  
Vol 87 (11) ◽  
pp. 3307-3316 ◽  
Author(s):  
Min-Chul Kim ◽  
Yong-Kuk Kwon ◽  
Seong-Joon Joh ◽  
A. Michael Lindberg ◽  
Jun-Hun Kwon ◽  
...  
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Large Open Reading Frame ◽  
Protein Motif ◽  
Reading Frame ◽  
Protein Motifs ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis ◽  
The Family

Duck hepatitis virus type 1 (DHV-1) was previously classified as an enterovirus, based primarily on observed morphology and physicochemical properties of the virion. The complete nucleotide sequences of two strains (DRL-62 and R85952) of DHV-1 have been determined. Excluding the poly(A) tail, the genomes are 7691 and 7690 nt, respectively, and contain a single, large open reading frame encoding a polyprotein of 2249 aa. The genome of DHV-1 is organized as are those of members of the family Picornaviridae: 5′ untranslated region (UTR)–VP0–VP3–VP1–2A1–2A2–2B–2C–3A–3B–3C–3D–3′ UTR. Analysis of the genomic and predicted polyprotein sequences revealed several unusual features, including the absence of a predicted maturation cleavage of VP0, the presence of two unrelated 2A protein motifs and a 3′ UTR extended markedly compared with that of any other picornavirus. The 2A1 protein motif is related to the 2A protein type of the genus Aphthovirus and the adjacent 2A2 protein is related to the 2A protein type present in the genus Parechovirus. Phylogenetic analysis using the 3D protein sequence shows that the two DHV-1 strains are related more closely to members of the genus Parechovirus than to other picornaviruses. However, the two DHV-1 strains form a monophyletic group, clearly distinct from members of the genus Parechovirus.

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