Real-Time Near-Infrared Fluorescent Cholangiography During Robotic Single-Site Cholecystectomy

2015 ◽  
pp. 107-115
Author(s):  
Nicolas C. Buchs
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Single Site ◽  
Fluorescent Cholangiography

Real-time near-infrared (NIR) fluorescent cholangiography in single-site robotic cholecystectomy (SSRC): a single-institutional prospective study

2012 ◽  
Vol 27 (6) ◽  
pp. 2156-2162 ◽  
Author(s):  
Giuseppe Spinoglio ◽  
Fabio Priora ◽  
Paolo Pietro Bianchi ◽  
Francesco Saverio Lucido ◽  
Alessio Licciardello ◽  
...  
Keyword(s):  
Prospective Study ◽  
Real Time ◽  
Near Infrared ◽  
Single Site ◽  
Fluorescent Cholangiography ◽  
Robotic Cholecystectomy

scholarly journals Real-time near-infrared fluorescent cholangiography could shorten operative time during robotic single-site cholecystectomy

2013 ◽  
Vol 27 (10) ◽  
pp. 3897-3901 ◽  
Author(s):  
Nicolas C. Buchs ◽  
François Pugin ◽  
Dan E. Azagury ◽  
Minoa Jung ◽  
Francesco Volonte ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Operative Time ◽  
Single Site ◽  
Fluorescent Cholangiography

scholarly journals Near-infrared fluorescent cholangiography – real-time visualization of the biliary tree during elective laparoscopic cholecystectomy

HPB ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 538-545 ◽  
Author(s):  
Antonio Pesce ◽  
Saverio Latteri ◽  
Martina Barchitta ◽  
Teresa R. Portale ◽  
Biagio Di Stefano ◽  
...  
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Real Time ◽  
Near Infrared ◽  
Biliary Tree ◽  
Fluorescent Cholangiography ◽  
Real Time Visualization ◽  
Elective Laparoscopic Cholecystectomy

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe

Talanta ◽  
2021 ◽  
Vol 228 ◽  
pp. 122184
Author(s):  
Qingfeng Xia ◽  
Shumin Feng ◽  
Jiaxin Hong ◽  
Guoqiang Feng
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Stress ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Real Time Tracking ◽  
Emissive Probe

scholarly journals P11: NEAR INFRARED FLUORESCENT CHOLANGIOGRAPHY IN LAPAROSCOPIC CHOLECYSTECTOMY: A SINGLE CENTRE FEASIBILITY STUDY. THE OPTIMUM DOSING REGIME, LIMITATIONS AND WHERE NEXT?

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
G Armstrong ◽  
G Toogood ◽  
DG Jayne ◽  
AM Smith
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Bile Duct ◽  
Indocyanine Green ◽  
Feasibility Study ◽  
Near Infrared ◽  
Intravenous Dose ◽  
Structure Identification ◽  
Dosing Regimen ◽  
Single Centre ◽  
Fluorescent Cholangiography

Abstract Introduction This study explored near-infrared fluorescent cholangiography (NIRFC) with Indocyanine Green (ICG) during laparoscopic cholecystectomy (LC) surgery in a tertiary referral hepatobiliary unit. ICG binds to albumin and is excreted in bile. NIRFC utilises the fluorescent and excretory properties of ICG to provide dynamic extrahepatic bile duct mapping during LC. Method Non-randomised single centre feasibility study. Twenty-two participants were sequentially allocated to four dosing subgroups prior to NIRFC assisted LC. Each received a single intravenous dose of ICG prior to LC with the Stryker Novadaq NIR laparoscope. The biliary anatomy was assessed with NIRFC at three time-points, detection was compared to radiological cholangiogram where available and surgeon satisfaction was assessed. Result Eight participants received 2.5mg ICG 20-40min before surgery, four 0.25mg/kg 20-40min, five 90min – 180min and five 12 – 36 hour pre-operatively. Average age 50 years (S.D±15), BMI 27.5m2 (S.D±3.6), 6/22 were acute LC procedures. The prolonged dosing interval produced increased extrahepatic biliary structure identification (p = 0.016), reduced noise to signal ratio and was consistently preferred by the operating surgeon. NIRFC was inferior to radiological cholangiogram (n = 10) (p = 0.014) for bile duct mapping. We observed iatrogenic bile spillage saturating the field and obscuring structure differentiation and peri-hilar inflammation impeding fluorescent detection in acute LC. Conclusion The dosing regimen 0.25mg/kg ICG 12 to 36 hours prior to surgery provides optimum NIRFC structure visualisation. Fluorescent tissue penetrance is limited in acute peri-hilar inflammation. More research in to the efficiency of NIRFC in emergency LC is required. Take-home message An intravenous dose of 0.25mg/kg of Indocyanine Green 12 to 36 hours before surgery is the optimum dosing regimen for increased extra-hepatic bile duct structures with near infrared fluorescent cholangiography. The role of NIRFC in acute laparoscopic cholecystectomy surgery remains ill-defined.

Near-Infrared Off-Axis Integrated Cavity Output Spectroscopic Gas Sensor for Real-Time, In Situ Atmospheric Methane Monitoring

2020 ◽  
pp. 1-1
Author(s):  
Kaiyuan Zheng ◽  
Chuantao Zheng ◽  
Haipeng Zhang ◽  
Junhao Li ◽  
Zidi Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Gas Sensor ◽  
Near Infrared ◽  
Atmospheric Methane ◽  
Cavity Output

Kinetic and mechanistic studies on the Heck reaction using real-time near infrared spectroscopy

2003 ◽  
Vol 5 (20) ◽  
pp. 4455-4460 ◽  
Author(s):  
Susana C. Cruz ◽  
Petra J. Aarnoutse ◽  
Gadi Rothenberg ◽  
Johan A. Westerhuis ◽  
Age K. Smilde ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Near Infrared Spectroscopy ◽  
Heck Reaction ◽  
Near Infrared ◽  
Mechanistic Studies
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