Optimization of Biosurfactant Production and Crude Oil Emulsification by Bacillus Sp. Isolated from Hydrocarbon Contaminated Soil Sample

Author(s):  
P. Kanmani ◽  
E. DivyaSri ◽  
R. Rajakarvizhi ◽  
O. S. Senthamil ◽  
V. Sivasankari ◽  
...  
2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.


2018 ◽  
Vol 6 (4) ◽  
pp. 4347-4356 ◽  
Author(s):  
C. Vigneshwaran ◽  
V. Sivasubramanian ◽  
K. Vasantharaj ◽  
N. Krishnanand ◽  
M. Jerold

2019 ◽  
Vol 7 (2) ◽  
pp. 30-34
Author(s):  
Ajay Kumar Sahu ◽  
Rahul Nemani ◽  
Prangya Prangya Acharya ◽  
Rupali Sinha ◽  
Subhranil Sengupta ◽  
...  

The isolation of oil contaminated sites and gravimetric analysis of degradation in which, two bacterial formed maximum clearing zones on minerals salt medium. An increase in cell number indicating that the bacterial isolation was responsible for the oil degradation .the collected oil contaminated sites at kormongala, Indra nagar, MTTC culture , micrococcus spp., Bacillus spp., pseudomonas spp., which are able to utilize the oil in soil as carbon sources, were added to oil contaminated soil sample , the growth profiles were determined by monitoring the optical density, dry weight and Ph of the culture utilizing lubricating oil as sole sources of carbon, Bushnell  Haas media supplemented with petrol, kerosene and diesel as sole carbon sources was used for isolation of bacteria capable of degrading these petroleum fractionates. From three soil sample and two water sample, a total of nine bacterial strains were isolated capable of degrading petrol, kerosene and diesel with varying tolerance capacities, the isolates were identify by using standard biochemical test and morphological studies and it was determined that these strains belong to six bacterial genera .the present study suggest that the isolated bacterial species could be employed for bioremediation in environment polluted with petroleum and its products, indigenously from the soil and water contaminated with crude oil in the vicinity of oil drilling well were found to be most efficient crude oil utilize as turbidity observed by spectrophotometrically. In the various study of lipolytic bacteria concluded that the taken of oil contaminated soil from from petroleum bunk and to identify their biochemical characterization by using various sources. How it’s helpful for characterize by using of lipolytic bacteria


2018 ◽  
Vol 23 (4) ◽  
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA

<p>This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: <em>Bacillus substilis</em> and <em>Pseudomonas sp</em>. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5    (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.</p>


2014 ◽  
Vol 16 (4) ◽  
pp. 897-903 ◽  
Author(s):  
Mutai Bao ◽  
Yongrui Pi ◽  
Lina Wang ◽  
Peiyan Sun ◽  
Yiming Li ◽  
...  

In this work, a hydrocarbon-degrading bacterium D3-2 isolated from petroleum contaminated soil samples was investigated for its potential effect in biodegradation of crude oil. The strain was identified as Acinetobacter sp.


2015 ◽  
Vol 65 (4) ◽  
pp. 2255-2264 ◽  
Author(s):  
Hangcheng Zhou ◽  
Jixiang Chen ◽  
Zhi Yang ◽  
Bo Qin ◽  
Yanlin Li ◽  
...  

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase


2020 ◽  
Vol 57 (6) ◽  
pp. 515-520
Author(s):  
Nayyereh Kholghi ◽  
Hossein Amani ◽  
Shokoufeh Malekmahmoodi ◽  
Alireza Amiri

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