KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

scholarly journals Microbial exploration of the origin of the Pandeglang cocoa plant (Theobroma cacao L.) rhizosphere as potential biofertilizer

2020 ◽  
Vol 8 (2) ◽  
pp. 2611-2616
Author(s):  
N Nurmayulis ◽  
Fitria Riany Eris ◽  
Dewi Hastusi ◽  
Abdul Hasyim Sodiq ◽  
Yus Rama Denny ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Rhizosphere Soil ◽  
Biological Agents ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Hemolysis Test ◽  
Phosphate Solubilizing ◽  
Theobroma Cacao L ◽  
Initial Selection

This study aimed to determine the ability of microorganisms originating from the root ecosystem (Rhizosphere) of cocoa (Theobroma cacao L) as a potential superior to biological agents. For the experiment, soil samples were observed from the rhizosphere of cocoa plants in Pandeglang Regency. At this stage, the research was focused on finding out the extent of microbe screening results from cocoa rhizosphere soil in producing phytohormones. The results from the initial selection showed a population of Azotobacter 2.8 x 105 cfu/mL, a population of Azospirillium of 0.3 x 102 cfu/mL, and a population of Phosphate-solubilizing bacteria (PSB) 4.1 x 107 cfu/mL. In the hemolysis test conducted for 9 isolates, only 1 isolate showed a negative result. From the test of the ability to produce phytohormones showed respectively from the highest values were IAA 5.467 mg/L, gibberellin 3.768 mg/L, zeatin 1.321 mg/L, and kinetin 0.886 mg/L. The land rhizosphere of Pandeglang cocoa plant had superior potential microbes which could be used as biological fertilizers.

scholarly journals Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa

2012 ◽  
Vol 17 (1) ◽  
pp. 43 ◽  
Author(s):  
Jimena Paola Angulo-Cortés ◽  
Anamaría García-Díaz ◽  
Aura Marina Pedroza ◽  
María Mercedes Martínez-Salgado ◽  
Viviana Gutiérrez-Romero
Keyword(s):  
Phosphatase Activity ◽  
Microbial Growth ◽  
Culture Media ◽  
Biomass Concentration ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Box Behnken Design ◽  
Phosphate Solubilizing ◽  
Complex Culture

<strong>Objective</strong>. To design a complex culture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. <strong>Materials</strong> <strong>and methods</strong>. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which were then assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied to<br />evaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratory scale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. <strong>Results</strong>. Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and 2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect on the phosphatase activity (p&lt;0.01) was hydrolyzed yeast, and the formulation that generated the highest biomass concentration and phosphatase activity (p&lt;0.01) contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24 hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFU and 12.9 phosphatase units. <strong>Conclusion</strong>. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5 gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, we proved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases.<br /><br /><strong>Key words</strong>: bio-inoculants, phosphate solubilizing microorganisms, phosphatase activity, Box Behnken design.

scholarly journals Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

2010 ◽  
Vol 12 (3,4) ◽  
pp. 231 ◽  
Author(s):  
M. Ogut ◽  
F. Er ◽  
N. Kandemir
Keyword(s):  
Phosphate Solubilization ◽  
Soil Phosphorus ◽  
Soil Samples ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Batch Cultures ◽  
Phosphate Solubilizers ◽  
Gene Sequence Analysis ◽  
Initial Ph ◽  
Phosphate Solubilizing

<p>Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, <em>Acinetobacter</em> (7), <em>Pseudomonas</em> (7), <em>Enterobacter</em> (2), <em>Enterococcus</em> (1), <em>Escherichia</em> (1), <em>Photorhabdus</em> (1), and <em>Bacillus</em> (1) as determined by the 16S rDNA gene sequence analysis. Since the <em>Acinetobacter</em> species were most effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8  dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. <em>Acinetobacter</em> isolates have some potential as an inoculum both for soil and biotechnological P-solubilization.</p>

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

scholarly journals ISOLATION AND CHARACTERISTICS OF PHOSPHATE SOLUBILZING BACTERIA (PSB) FROM VEGETATIVE AND GENERATIVE PHASE OF MAIZE RHIZOSPHERE

2020 ◽  
Vol 7 (2) ◽  
pp. 53-60
Author(s):  
Fany Juliarti Panjaitan
Keyword(s):  
Zea Mays L ◽  
Soil Samples ◽  
P Value ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Anion ◽  
Maize Rhizosphere ◽  
Generative Phase ◽  
Phosphate Solubilizing ◽  
Biological Fertilizer ◽  
Number Of Cells

The plants acquire phosporus from soil solution as phosphate anion. The availability of nutrients is very low in soil and crops compared to the other macronutriens. It precipitates in soil as orthophosphate or absorbed by Al and Fe so that inhibiting the plant growth. Phosphate solubilizing bacteria are able to release the P bond of clay minerals and provide it for crops. The research aimed to get phosphate solubilzing microbes from maize (Zea mays L.) rhizosphere. The soil samples were taken from the maize rhizosphere in both the vegetative and generative phases in the Cikabayan Bogor experimental farm. The phosphate solubilzing bacteria were determined for its ability to dissolve phosphate in liquid Pikovskaya media. The results of research were obtained 16 phosphate solubilizing bacteria, each of the 12 isolates derived from maize rhizosphere in vegetative phase (JM FIO) and 4 isolates in generative phase (JT FIO). The phosphate solubiliton index of each phosphate solubilizing bacteria was varied, namely 2,2-4, the largest dissolution index obtained at JM FIO 1. The largest phosphate dissolving ability in liquid Pikovskaya media was showed by JM FIO 3 isolate, P value was 0,60 ppm or increased 300% of control then followed by JM FIO 9 with 0,43 ppm P. The research also showed that JM FIO 3 and JM FIO 9 were not pathogenic and potentially could be used as biological fertilizer with number of cells at each 4.2 x 109 and 1.2 x 109 CFU/g of carrier.Key Words : Phosphate Solubilizing Microbe, Maize, Rhizosphere

scholarly journals Phosphate Solubilization Potential of Rhizosphere Fungi Isolated from Plants in Jimma Zone, Southwest Ethiopia

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Firew Elias ◽  
Delelegn Woyessa ◽  
Diriba Muleta
Keyword(s):  
Agricultural Production ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Soil Samples ◽  
Maximum Amount ◽  
Southwest Ethiopia ◽  
Rhizosphere Fungi ◽  
Jimma Zone ◽  
Phosphate Solubilizing ◽  
Diverse Plant

Phosphorus (P) is one of the major bioelements limiting agricultural production. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid Pikovskaya (PVK) medium. A total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and sugarcane. Among the isolates, 167 (46.52%) solubilized inorganic phosphate. The isolated phosphate solubilizing fungi belonged to genera ofAspergillus(55.69%),Penicilliumspp. (23.35%), andFusarium(9.58%). Solubilization index (SI) ranged from 1.10 to 3.05. Isolates designated as JUHbF95 (Aspergillussp.) and JUFbF59 (Penicilliumsp.) solubilized maximum amount of P 728.77 μg·mL−1and 514.44 μg mL−1, respectively, from TCP (tricalcium phosphate) after 15 days of incubation. The highest (363 μg mL−1) soluble-P was released from RP with the inoculation of JUHbF95 in the PVK broth after 10 days of incubation. The present study indicated the presence of diverse plant associated P-solubilizing fungi that may serve as potential biofertilizers.

scholarly journals Co-Inoculation of Mesorhizobium ciceri with Either Bacillus sp. or Enterobacter aerogenes on Chickpea Improves Growth and Productivity in Phosphate-Deficient Soils in Dry Areas of a Mediterranean Region

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 571
Author(s):  
Imane Benjelloun ◽  
Imane Thami Alami ◽  
Mohamed El Khadir ◽  
Allal Douira ◽  
Sripada M. Udupa
Keyword(s):  
Field Experiments ◽  
Enterobacter Aerogenes ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Mesorhizobium Ciceri ◽  
Shoot Dry Weight ◽  
Combined Application ◽  
Dry Areas ◽  
Phosphate Solubilizing

Biological nitrogen fixation requires a large amount of phosphorus (P). However, most of the soils are P-deficient and the extensive use of P- chemical fertilizers constitute a serious threat to the environment. In this context, two field experiments were carried out to investigate the effect of co-inoculation of Mesorhizobium ciceri with phosphate solubilizing bacteria (PSB), Bacillus sp., and Enterobacter aerogenes, on chickpea as an alternative to chemical nitrogen (N) and phosphorous fertilizers in P-deficient soils in dry areas of Morocco. The results revealed that combined inoculation of chickpea with rhizobia and PSB showed a significant enhancement of chickpea nodulation, biomass production, yields and N, P, and protein content in grains as compared to single inoculation or single application of N or P. A significantly higher increase was obtained by inoculating chickpea with Mesorhizobium sp. MA72 combined with E. aerogenes P1S6. This combination allowed an enhancement of more than 270% in nodulation, 192% in shoot dry weight and 242% in grain yield. The effect of this combination was equivalent to the effect of combined application of N and P fertilizers. Formulation of biofertilizers based on tasted strains could be used for chickpea co-inoculation in P-deficient soils for an eco-friendly sustainable production of chickpea.

scholarly journals Identifikasi dan Kuantifikasi Metabolit Bakteri Pelarut Fosfat dan Pengaruhnya terhadap Aktivitas Rhizoctonia solani pada Tanaman Kedelai

2018 ◽  
Vol 13 (3) ◽  
pp. 233-240 ◽  
Author(s):  
Tri Candra Setiawati ◽  
Paniman Asna Mihardja
Keyword(s):  
Alkaline Phosphatase ◽  
Organic Acids ◽  
Rhizoctonia Solani ◽  
Total Concentration ◽  
Phosphate Solubilizing Bacteria ◽  
Soil Medium ◽  
Second Stage ◽  
Third Stage ◽  
Phosphate Solubilizing

Phosphate solubilizing bacteria (PSB) metabolites are organic acids, phosphomonoesterase enzyme (alkaline phosphatase) and antibiotic, which is able to dissolve insoluble phosphate. Phosphate solubilizing bacteria used in this study was expected to suppress Rhizoctonia solani attacks. This experiment was aimed at (1)  identifiying and quantifying  PSB metabolites, and (2) examining their capability as biocontrol agent for Rhizoctonia solani in vitro and hydroponics soybean. This study was conducted in three stages. The first stage of this study was culturing two PSB isolates (Pseudomonas putida 27.4B and Pseudomonas diminuta) in the Pikovskaya medium to analyze their metabolites. The second and third stage of this study was testing the antagonist of two bacteria to suppressed R. solani activity, which was conducted in vitro, and in hydroponics medium soybean as indicator plant. The results showed that P. putida 27.4B and P. diminuta produced organic acids i.e.: citrate, formic, succinic, acetic, propionate, butyrate, and oxalate. The totals of organic acids from each bacterium were 70,3 mg.kg-1 and 61,9 mg.kg-1. Production of alkaline phosphatase enzyme in Pikovskaya medium of P. Putida27.4B was 11,71 μg pNP .mL-1.h-1 and P. diminuta was 24,04  μg pNP.mL-1.h-1. Concentration of this enzyme in soil medium was higher than that in Pikovskaya medium with 26,27 μg pNP.g-1.h-1 and 39,03 μg pNP.g-1.h-1 respectively. This study also showed that total concentration of antibiotics (tetracycline, oxitetracycline and penicillin) produced by the PSB, were 3,2 μg.mL-1 (P. putida 27.4B) and 10,96 μg.m1-1 (P. diminuta), respectively. The results from second stage of this study showed that by using in vitro, the reduced growth of  R. solani was observed 58,35% with P. putida 27.4B and 41,96% with P. diminuta. In addition, inoculations of PSB in hydroponics medium reduced the fungal pathogenesis from 10,71% to 21,42% of pre and post emergence damping-off. Visually, the symptom of pathogen attack appeared within the period of  2 untill 14 days after infection.

Sign in / Sign up

Export Citation Format

Share Document