Investigation on Heavy Metal Removal from a Crude Oil Contaminated Soil using Rhamnolipid Biosurfactant as a New Eco-Friendly Method

Soil contaminated with Cd and Pb has caused sharp decrease of cultivatable soil and has been attracting increasing attention. Biosurfactants are efficient in solving the problem. However, little information is available about the influence of sophorolipids (SLs) on the remediation of Cd- or Pb-contaminated soil. The sophorolipids produced by Starmerella bombicola CGMCC 1576 were used to study the effects of Cd and Pb removal in batch soil washing from artificially contaminated soil. The removal efficiency of crude total SLs was better than both distilled water and synthetic surfactants. Furthermore, 83.6% of Cd and 44.8% of Pb were removed by 8% crude acidic SLs. Acidic SLs with high water solubility were more effective than lactonic SLs in enhancing remediation of heavy metal-contaminated soils. The complexation of Cd with the free carboxyl group of the acidic SLs was observed by Fourier-transform infrared spectroscopy study, and this complexation was effective in heavy metal removal from the soil. The fermentation broth of S. bombicola, without further preparation, removed 95% of Cd and 52% of Pb. These results suggested that SLs produced by S. bombicola could function as potential bioremediation agents for heavy metal-contaminated soil.

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Heavy metal removal competence of individual and bacterial consortium, evolved from metal contaminated soil

Materials Today Proceedings ◽

10.1016/j.matpr.2021.01.566 ◽

2021 ◽

Author(s):

Ponniah Anusha ◽

Devarajan Natarajan ◽

Mathiyazhagan Narayanan

Keyword(s):

Heavy Metal ◽

Contaminated Soil ◽

Metal Removal ◽

Heavy Metal Removal ◽

Bacterial Consortium

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Remediation of Crude Oil Contaminated Soil, Using Organic Supplement: Effects on Growth and Heavy Metal Uptake in Cassava (Manihot esculenta Crantz)

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v25i1.1 ◽

2021 ◽

Vol 25 (1) ◽

pp. 5-14

Author(s):

S.R. Osu ◽

I.R. Udosen ◽

G.E. Udofia

Keyword(s):

Heavy Metal ◽

Crude Oil ◽

Contaminated Soil ◽

Metal Uptake ◽

Field Experiments ◽

Metal Removal ◽

Poultry Manure ◽

Heavy Metal Uptake ◽

Manihot Esculenta Crantz ◽

Growth Properties

Field experiments were conducted in 2016 and repeated in 2017 to evaluate the effectiveness of Oil Palm Bunch Ash (OPBA) and Dried Poultry manure (DPM) as organic supplement, applied singly and in combination on growth properties and heavy metal uptake of cassava cultivars grown in crude-oil contaminated soil of the Niger Delta Region, Nigeria. There were four different organic amendments viz: DPM, OPBA, OPBA +DPM and control using TMS 30572, NR8082 and local variety as test crops. Results were elaborated statistically with the use of three-by-four factor variance analysis with ANOVA. Effect of various application of amendment on morphometric parameters of cultivars revealed in 2017 that at 36 weeks after sprouting (WAS), DPM and OPBA +DPM significantly (P≤ 0.05) enhanced plant heights of TMS 3572 and LV but had no influence on number of nodes, stem girth and leaf area. However, the interactions resulted in significant (P≤ 0.05) uptake and concentration of the heavy metals Fe, Cu, and Pb in leaves and Fe in roots ofTMS30572 and NR8082, indicating efficient metal removal by these varieties. Results of amendment also revealed remarkable increment in bacterial and fungal (from 104 to 106 and 103 to 104 ) populations respectively, correlating with reduction in soil total petroleum hydrocarbon (TPH) from 156.45 mg/kg to 146.73 mg/Kg. This is observed to diminish oil toxicity and improve soil status. The response of these cassava varieties to crude oil contamination appears to be optimistic. Consequently crop farmers are adviced against planting cassava and other arable crop at crude oil impacted soil for the risk of uptake of heavy metal in crop tissues. Keywords: Remediation, Heavy metal, Oil-contaminated soil, Organic amendment, Growth properties, Cassava

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Heavy Metal Removal from a Contaminated Soil using Chemical Reagents

International Journal of Engineering Research and ◽

10.17577/ijertv6is020350 ◽

2017 ◽

Vol V6 (02) ◽

Author(s):

Susan K Santy ◽

Febina A Manaf ◽

Keyword(s):

Heavy Metal ◽

Contaminated Soil ◽

Metal Removal ◽

Heavy Metal Removal ◽

Chemical Reagents

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Bioremediation of heavy metals from artisanal crude oil refinery (Kpo-Fire) impacted soil using Bacillus flexus and Pseudomonas aeruginosa in Ngie Community, Degema Local Government Area, Rivers State, Nigeria

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v24i12.6 ◽

2021 ◽

Vol 24 (12) ◽

pp. 2049-2054

Author(s):

C.J. Ugboma ◽

T. Sampson ◽

N.E. Mbonu

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Pseudomonas Aeruginosa ◽

Contaminated Soil ◽

Metal Removal ◽

Heavy Metal Removal ◽

Oil Refinery ◽

Experimental Setup ◽

Strong Positive Correlation ◽

Bacillus Flexus

The existence of heavy metals in “kpo-fire” impacted soil creates significant risks to human health and the ecosystem. In this study, the efficacy of the elimination of heavy metal from “kpo-fire” impacted soil was evaluated using bacterial treatments. The organisms (Bacillus flexus and Pseudomonas aeruginosa) used in the bioremediation setup were isolated from the impacted soil. Heavy metal analysis was carried out using an Atomic Absorption Spectrophotometer. The experimental setup involved the recreation of the contaminated soil sample in three (3) vessels labeled as: Flask A containing 300g of un-amended sample (control) to monitor natural process; Flask B containing 300g of sterilized sample; Flask C containing 300g of sample with Pseudomonas aeruginosa and Bacillus flexus. Soil baseline physicochemical composition was determined to have a pH of 6.18, Temperature of 29.2oC, Total Organic Carbon 7.58 mg/kg and Phosphate concentration 37.56 mg/kg. At the end of the investigation, experimental setup C, containing bacterial inocula was observed to possess the best bio-removal rates for Mercury (99.32%), Cadmium (77.59%), Boron (72.84%) and Arsenic (93.43%) after a 42-day period of study. Also, the concentrations declined from 1.05264mg/kg to 0.00621mg/kg for Mercury; Cadmium declined from 5.93mg/kg to 1.16mg/kg; Boron declined from 3.61mg/kg to 0.82mmg/kg and Arsenic declined from 2.78mg/kg to 0.16mg/kg. Molecular characterization revealed the contaminated soil had predominance of isolates with genomic molecular weight of 1,500 bp and the phylogenetic construct showed the bacterial isolates were Pseudomonas aeruginosa (MT023359), Bacillus flexus (MT023375) and Lysinibacillus macroides (MT023377). Statistical analysis revealed a strong positive correlation between the bacterial biomass and heavy metal removal. The synergistic parts played by bacterial consortia in the bio-removal of heavy metals from the polluted soil have been established and these potentials can be harnessed as a roadmap for eco-recovery of impacted environment in the Niger Delta. Bacillus flexus and Pseudomonas aeruginosa in consortium are efficient in remediation of kpo-fire contaminated soil.

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Production of a non-cytotoxic bioflocculant by a bacterium utilizing a petroleum hydrocarbon source and its application in heavy metal removal

RSC Advances ◽

10.1039/c5ra08636a ◽

2015 ◽

Vol 5 (81) ◽

pp. 66037-66046 ◽

Cited By ~ 21

Author(s):

M. Pathak ◽

A. Devi ◽

K. G. Bhattacharyya ◽

H. K. Sarma ◽

S. Subudhi ◽

...

Keyword(s):

Heavy Metal ◽

Carbon Source ◽

Activated Sludge ◽

Crude Oil ◽

Petroleum Hydrocarbon ◽

Metal Removal ◽

Heavy Metal Removal ◽

Oil Refinery

A bacterium isolated from the activated sludge of an oil refinery of Assam, India retained efficient bioflocculating activity through production of the bioflocculant when it was grown on a crude oil amended medium void of any other carbon source.

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