Aqueous Two-Phase System Strategies for the Recovery and Partial Purification of Bioactive Low Molecular Weight Compounds

2017 ◽  
pp. 79-96 ◽  
Author(s):  
Juan Carlos Sánchez-Rangel ◽  
Daniel A. Jacobo-Velázquez ◽  
Jorge Benavides
Keyword(s):  
Molecular Weight ◽  
Partial Purification ◽  
Low Molecular Weight ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Low Molecular Weight Compounds

scholarly journals A novel and environmentally friendly bioprocess for separation and partial purification of polysaccharides from Cordyceps sinensis mycelia by an aqueous two-phase system

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37659-37665 ◽  
Author(s):  
Zhigang Li ◽  
Ailin Chen ◽  
Zhencheng Li ◽  
Man Qu ◽  
Huayong Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Environmentally Friendly ◽  
Cordyceps Sinensis ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P ◽  
Active Substances

An aqueous two-phase system comprising ethanol and salt not only could purify CS polysaccharides with high efficiency, but also simultaneously separated multiple active substances through coupling with ultrafiltration.

Partial purification of soluble potato polyphenol oxidase by partitioning in an aqueous two-phase system

1993 ◽  
Vol 41 (8) ◽  
pp. 1219-1224 ◽  
Author(s):  
Alvaro. Sanchez-Ferrer ◽  
Francisco. Laveda ◽  
Francisco. Garcia-Carmona
Keyword(s):  
Polyphenol Oxidase ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

2017 ◽  
Vol 52 (5) ◽  
pp. 834-842 ◽  
Author(s):  
Hadi Sofijan ◽  
Tan Joo Shun ◽  
Sahar Abbasiliasi ◽  
Shuhaimi Mustafa ◽  
Nyoman Tri Puspaningsih ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Recovery and partial purification of fibrinolytic enzymes of Auricularia polytricha (Mont.) Sacc by an aqueous two-phase system

2014 ◽  
Vol 122 ◽  
pp. 359-366 ◽  
Author(s):  
Sharjahan Mohamed Ali ◽  
Tau Chuan Ling ◽  
Sekaran Muniandy ◽  
Yee Shin Tan ◽  
Jegadeesh Raman ◽  
...  
Keyword(s):  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Fibrinolytic Enzymes ◽  
Auricularia Polytricha ◽  
Aqueous Two Phase System

Extraction of protease from ora‐pro‐nobis ( Pereskia aculeata Miller) and partial purification in polyethylene glycol + sodium phosphate aqueous two‐phase system

2021 ◽  
Author(s):  
Annie Nolasco Alves ◽  
Priscilla Amaral Nascimento ◽  
Rafael da Costa Ilhéu Fontan ◽  
Evaldo Cardozo de Sousa Júnior ◽  
Paulo Bonomo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Phosphate ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Application of Aqueous Two-Phase Systems for the Recovery of Bioactive Low-Molecular Weight Compounds

2014 ◽  
Vol 49 (12) ◽  
pp. 1872-1882 ◽  
Author(s):  
Jesús Simental-Martínez ◽  
Bertha Montalvo-Hernández ◽  
Marco Rito-Palomares ◽  
Jorge Benavides
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Two Phase ◽  
Low Molecular Weight Compounds ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Utilization of Integrative Technique for Partial Recovery of Proteases from Soil Microbes

2018 ◽  
Author(s):  
Said Nawab ◽  
Muhammad Aasim ◽  
Haris Saddique ◽  
Fazal Rabi ◽  
Waqar Ali ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Partition Coefficient ◽  
Cost Effective ◽  
Maximum Activity ◽  
Partial Purification ◽  
Phase System ◽  
Partial Recovery ◽  
Two Phase ◽  
Peg 4000 ◽  
Partitioning Behavior

Aqueous two-phase system (ATPS) is an efficient, cost effective, fast, simple and ecofriendly method for the recovery of biomolecules. In the present study, an ATPS composed of polyethylene glycol and ammonium sulphate (NH4)2SO4 was used for the partial purification of proteases from microbial source. The effects of different parameters such as molecular weight of PEG (4000, 6000 and 10000), concentration of PEG (15, 17.5 and 20 %) and concentration of (NH4)2SO4 (7.5, 8.3, 9.1 and 9.9 %) on the partitioning behavior of proteases at room temperature were investigated. Generally, increasing the concentration of PEG and (NH4)2SO4 moved the protease to the top i.e., polymer-rich phase. Increasing the molecular weight of PEG from 4000 to 10000 the partition coefficient decreased subsequently. The highest partition coefficient i.e., 3.32 and maximum activity i.e., 16.06 soxhlet unit was found in an optimum system composed of 20 % PEG 4000 and 9.9 % (NH4)2SO4.

Partial purification of fibrinolytic and fibrinogenolytic protease from Gliricidia sepium seeds by aqueous two-phase system

2020 ◽  
Vol 27 ◽  
pp. 101669 ◽  
Author(s):  
Aníbia Vicente da Silva ◽  
Jéssica Miranda do Nascimento ◽  
Cláudio Henrique Rodrigues ◽  
Débora Cristina Silva Nascimento ◽  
Romero Marcos Pedrosa Brandão Costa ◽  
...  
Keyword(s):  
Gliricidia Sepium ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Low Molecular Weight Microfibers with Light Sensing Properties

2017 ◽  
Vol 54 (4) ◽  
pp. 655-658
Author(s):  
Andrei Bejan ◽  
Dragos Peptanariu ◽  
Bogdan Chiricuta ◽  
Elena Bicu ◽  
Dalila Belei
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
X Ray Diffraction ◽  
Aromatic Rings ◽  
X Ray ◽  
Force Microscopy ◽  
Light Sensing ◽  
Sensing Applications ◽  
Atomic Force ◽  
Low Molecular Weight Compounds

Microfibers were obtained from organic low molecular weight compounds based on heteroaromatic and aromatic rings connected by aliphatic spacers. The obtaining of microfibers was proved by scanning electron microscopy. The deciphering of the mechanism of microfiber formation has been elucidated by X-ray diffraction, infrared spectroscopy, and atomic force microscopy measurements. By exciting with light of different wavelength, florescence microscopy revealed a specific optical response, recommending these materials for light sensing applications.

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