Extraction of protease from ora‐pro‐nobis ( Pereskia aculeata Miller) and partial purification in polyethylene glycol + sodium phosphate aqueous two‐phase system

2021 ◽  
Author(s):  
Annie Nolasco Alves ◽  
Priscilla Amaral Nascimento ◽  
Rafael da Costa Ilhéu Fontan ◽  
Evaldo Cardozo de Sousa Júnior ◽  
Paulo Bonomo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Phosphate ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate

2008 ◽  
pp. 171-180 ◽  
Author(s):  
Draginja Pericin ◽  
Senka Madjarev-Popovic ◽  
Zuzana Vastag
Keyword(s):  
Polyethylene Glycol ◽  
Factorial Design ◽  
Separation Factor ◽  
Sodium Phosphate ◽  
Acid Protease ◽  
Phase System ◽  
Penicillium Roqueforti ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Peg 4000

The partitioning of acid protease produced by submerged fermentation of Penicillium roqueforti in the aqueous two-phase system polyethylene glycol/sodium phosphate was studied. In the system with 15% polyethylene glycol 4000/25% NaH2PO4 favourable partition coefficient and the top phase yield were obtained, 2.651 and 56.51%, respectively. The purification of acid protease from crude enzyme was optimized by optimizing separation factor. Completely randomized factorial design was applied and the system 15.54% PEG 4000/20% NaH2PO4 appeared to be optimal. Statistically calculated separation factor of 3.553, was similar to the experimentally obtained value (3.084), which indicates the possible application of the factorial design onto ATPS partition and purifycation.

Polyethylene glycol-modified avidin: A novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system

1996 ◽  
Vol 7 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Hiroyuki Nishimura ◽  
Nobuhiro Munakata ◽  
Konosuke Hayashi ◽  
Masao Hayakawa ◽  
Hideki Iwamoto ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complex ◽  
Selective Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Novel Agent

Aqueous Two-Phase Extraction of Potassium Glycyrrhizinate with Polyethylene Glycol/(NH4)2SO4

2012 ◽  
Vol 550-553 ◽  
pp. 1687-1690
Author(s):  
Chen Sun ◽  
Wen Ju Liu ◽  
Hai Ming Zhang
Keyword(s):  
Polyethylene Glycol ◽  
Mass Fraction ◽  
Ph Value ◽  
Phase System ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Result Show ◽  
A New Technique ◽  
Aqueous Two Phase Extraction

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

scholarly journals Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

2017 ◽  
Vol 133 ◽  
pp. 8-14 ◽  
Author(s):  
Maria Carolina de Albuquerque Wanderley ◽  
José Manoel Wanderley Duarte Neto ◽  
Wendell Wagner Campos Albuquerque ◽  
Daniela de Araújo Viana Marques ◽  
Carolina de Albuquerque Lima ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Phase System ◽  
Purification And Characterization ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Penicillium Sp
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