The purpose of this study was to develop a functional microscope system
to overcome the inherent disadvantages of using the light microscope/camera
lucida system to reconstruct sections of biological material. The problem is
perhaps most acute in the nervous system, where reconstruction of
3-dimensional neurons or the tracing of neuronal pathways over extensive
distances must be attempted. The camera lucida system suffers from many
inaccuracies and is an extremely time-consuming and tedious
procedure.
Point projection x-ray microscopy in the SEM, entails utilizing the
primary electron beam in the spot mode to generate a point source of x-rays
from a suitable target (Al, Cu, Au). This technique was first successfully
demonstrated by Horn and Waltinger (1978).