Skeletal Tracer Kinetics: Science and Practice

2012 ◽  
pp. 85-107
Author(s):  
Glen M. Blake ◽  
Michelle Frost ◽  
Amelia E. B. Moore ◽  
Muhammad Siddique ◽  
Ignac Fogelman
Keyword(s):  
Tracer Kinetics

IC-P-112: Bias correction for linear simplifications of tracer kinetics with application in fibrillar amyloid beta measurements

2009 ◽  
Vol 5 (4S_Part_2) ◽  
pp. P46-P47
Author(s):  
Hongbin Guo ◽  
Rosemary A. Renaut ◽  
Kewei Chen ◽  
Eric Reiman
Keyword(s):  
Amyloid Beta ◽  
Bias Correction ◽  
Tracer Kinetics

Uptake and tracer kinetics of O-(2-18F-fluoroethyl)-l-tyrosine in meningiomas: preliminary results

2014 ◽  
Vol 42 (3) ◽  
pp. 459-467 ◽  
Author(s):  
Jan F. Cornelius ◽  
Gabriele Stoffels ◽  
Christian Filß ◽  
Norbert Galldiks ◽  
Philipp Slotty ◽  
...  
Keyword(s):  
Tracer Kinetics ◽  
Preliminary Results ◽  
Kinetics Of

scholarly journals Differential equation methods for simulation of GFP kinetics in non–steady state experiments

2018 ◽  
Vol 29 (6) ◽  
pp. 763-771 ◽  
Author(s):  
Robert D. Phair
Keyword(s):  
Differential Equation ◽  
Steady State ◽  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Fluorescent Proteins ◽  
Quantitative Information ◽  
Tracer Kinetics ◽  
Mathematical Framework ◽  
Experimental Protocols ◽  
Tracer Kinetic

Genetically encoded fluorescent proteins, combined with fluorescence microscopy, are widely used in cell biology to collect kinetic data on intracellular trafficking. Methods for extraction of quantitative information from these data are based on the mathematics of diffusion and tracer kinetics. Current methods, although useful and powerful, depend on the assumption that the cellular system being studied is in a steady state, that is, the assumption that all the molecular concentrations and fluxes are constant for the duration of the experiment. Here, we derive new tracer kinetic analytical methods for non–steady state biological systems by constructing mechanistic nonlinear differential equation models of the underlying cell biological processes and linking them to a separate set of differential equations governing the kinetics of the fluorescent tracer. Linking the two sets of equations is based on a new application of the fundamental tracer principle of indistinguishability and, unlike current methods, supports correct dependence of tracer kinetics on cellular dynamics. This approach thus provides a general mathematical framework for applications of GFP fluorescence microscopy (including photobleaching [FRAP, FLIP] and photoactivation to frequently encountered experimental protocols involving physiological or pharmacological perturbations (e.g., growth factors, neurotransmitters, acute knockouts, inhibitors, hormones, cytokines, and metabolites) that initiate mechanistically informative intracellular transients. When a new steady state is achieved, these methods automatically reduce to classical steady state tracer kinetic analysis.

Tracer Kinetics in Biomedical Research

2002 ◽  
Author(s):  
Claudio Cobelli ◽  
David Foster ◽  
Gianna Toffolo
Keyword(s):  
Biomedical Research ◽  
Tracer Kinetics

Ligand Tracer Kinetics: Theory and Application

2004 ◽  
pp. 75-93 ◽  
Author(s):  
Mark Slifstein ◽  
W. Gordon Frankle ◽  
Marc Laruelle
Keyword(s):  
Tracer Kinetics

Extracellular water measurements: organ tracer kinetics of bromide and sucrose in rats and man

1978 ◽  
Vol 235 (3) ◽  
pp. F254-F264 ◽  
Author(s):  
R. N. Pierson ◽  
D. C. Price ◽  
J. Wang ◽  
R. K. Jain
Keyword(s):  
Compartmental Model ◽  
Human Subjects ◽  
Water Volume ◽  
Tracer Kinetics ◽  
Sucrose Content ◽  
Extracellular Water ◽  
Tracer Injection ◽  
Diffusion Constants ◽  
Specific Organ ◽  
Kinetics Of

Bromide and sucrose distributions were measured as functions of time after tracer injection into 14 rat organs that accounted for 93% of body wt, with the goal of evaluating the use of bromide and sucrose as tracers for the extracellular water volume (ECW). The tracers, Na, 82Br, 125I-labeled human serum albumin, [14C]sucrose, and 3H2O, were used to calculate bromide and sucrose content in red cells, plasma, and 13 blood-free organs. Selective concentration of Br- occurs in RBC, stomach, and skin, accounting in part for the discrepancy between the Br- space and the smaller ECW volume as derived from other methods. Sucrose is rapidly metabolized in the rat and its 14C tracer cannot be used for ECW determination in this species. The kinetics of Br- distribution were estimated in rats and in 16 human subjects by measuring plasma disappearance values and specific organ uptakes. A pharmacokinetic compartmental model was derived, containing explicit parameters for blood flow, diffusion constants, and ECW spaces separately for each organ. Precise fitting of experimental bromide data was achieved for the rat; satisfactory fitting was also achieved in man from more limited plasma and biopsy data.

Modeling tracer kinetics in dynamic Gd-DTPA MR imaging

1997 ◽  
Vol 7 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Paul S. Tofts
Keyword(s):  
Mr Imaging ◽  
Tracer Kinetics

In vivo leucine oxidation in the gastrointestinal tract of lambs infected with nematode parasite Trichostrongylus colubriformis

1999 ◽  
Vol 1999 ◽  
pp. 107-107
Author(s):  
Feng Yu ◽  
L.A. Bruce ◽  
R.L. Coop ◽  
F Jackson ◽  
J.C. MacRae
Keyword(s):  
Gastrointestinal Tract ◽  
Tracer Kinetics ◽  
Isotope Tracer ◽  
Major Consequence ◽  
Nematode Parasites ◽  
Arterial Blood ◽  
First Pass Metabolism ◽  
First Pass ◽  
Pass Metabolism

One major consequence of the presence of the nematode parasites in the gastrointestinal tract (GIT) of ruminants appears to be an elevated flow of endogenous N component from the small intestine, leading to adverse changes in host productivity (MacRae, 1993). However, many of these aspects have remained speculative because of a lack of appropriate methodology to quantify the influence of parasites on GIT protein metabolism. In the present study oxidation of leucine sequestrated from arterial blood and digesta-derived leucine during “first pass” metabolism in the GIT of lambs subjected to subclinical T. colubriformis infection were quantified directly, using trans-organ catheterisation procedures coupled with stable isotope tracer kinetics.

Leucine metabolism across the gastrointestinal tract of sheep infected with Trichostrongylus colubriformis

1998 ◽  
Vol 1998 ◽  
pp. 1-1 ◽  
Author(s):  
Feng Yu ◽  
L.A. Bruce ◽  
R.L. Coop ◽  
J.C. MacRae
Keyword(s):  
Immune Response ◽  
Protein Synthesis ◽  
Gastrointestinal Tract ◽  
Protein Metabolism ◽  
Farm Animals ◽  
Tracer Kinetics ◽  
Trichostrongylus Colubriformis ◽  
Initial Infection ◽  
Leucine Metabolism ◽  
Isotope Tracer

In previous studies where sheep were subjected to experimental subclinical Trichostrongylus colubriformis infections, protein metabolism was seriously impaired during both the initial infection (5-7 weeks at early dosing) and the subsequent immune response (11-13 weeks of dosing) periods (see MacRae, 1993). Symonds and Jones (1983) reported that T. colubriformis infection increased the rates of protein synthesis in the small and large intestines of guinea pigs by 24 and 70% respectively, however there are no equivalent data in farm animals. In the present study trans-organ catheterisation procedures have been coupled with mass isotope tracer kinetics to examine leucine metabolism across the gastrointestinal (g.i.) tract of lambs subjected to subclinical T. colubriformis infection.

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