Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology

2009 ◽  
pp. 2337-2340
Author(s):  
T. Yagi ◽  
S. Wakasa ◽  
N. Tokunaga ◽  
Y. Akimoto ◽  
M. Umezu
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Speed ◽  
Real Time Imaging ◽  
Microfluidic Technology

Development of a high-speed VCSEL OCT system for real-time imaging of conscious patients larynx using a hand-held probe (Conference Presentation)

2016 ◽  
Author(s):  
Swathi Rangarajan ◽  
Li-Dek Chou ◽  
Carolyn Coughlan ◽  
Giriraj Sharma ◽  
Brian J. F. Wong ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Real Time Imaging

Application of single-cell real-time imaging flow cytometry in rapid detection of pathogenic fungi in clinical liquid specimens

2021 ◽  
Vol 9 (2) ◽  
pp. 025004
Author(s):  
Linting Lv ◽  
Li Dong ◽  
Jiajia Zheng ◽  
Tuohutaerbieke Maermaer ◽  
Xiangbo Huang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Real Time ◽  
Rapid Detection ◽  
Pathogenic Fungi ◽  
Real Time Imaging ◽  
Imaging Flow Cytometry

scholarly journals Single-cell real-time imaging of transgene expression upon lipofection

2016 ◽  
Vol 474 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Giuseppe Fiume ◽  
Carmine Di Rienzo ◽  
Laura Marchetti ◽  
Daniela Pozzi ◽  
Giulio Caracciolo ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Transgene Expression ◽  
Real Time Imaging

scholarly journals High-Speed, High-Performance Real-Time Imaging of Physiological Sarcomere Dynamics in the Beating Mouse Heart in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 463a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Takashi Ohki ◽  
Takako Terui ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

Real-time imaging of DNA–streptavidin complex formation in solution using a high-speed atomic force microscope

2007 ◽  
Vol 107 (2-3) ◽  
pp. 184-190 ◽  
Author(s):  
Mime Kobayashi ◽  
Koji Sumitomo ◽  
Keiichi Torimitsu
Keyword(s):  
Complex Formation ◽  
Atomic Force Microscope ◽  
Real Time ◽  
High Speed ◽  
Real Time Imaging ◽  
Atomic Force

scholarly journals Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

2020 ◽  
Author(s):  
Lang Zhou ◽  
Pengyu Chen ◽  
Aleksandr Simonian
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Intercellular Communication ◽  
Single Cells ◽  
Bone Marrow Failure ◽  
Cellular Protein ◽  
Label Free ◽  
Immune Effector ◽  
Real Time Imaging

Protein secretion of cells plays a vital role in intercellular communication. The abnormality and dysfunction of cellular protein secretion are associated with various physiological disorders, such as malignant proliferation of cells, aberrant immune function, and bone marrow failure. The heterogeneity of protein secretion exists not only between varying populations of cells, but also in the same phenotype of cells. Therefore, characterization of protein secretion from single cell contributes not only to the understanding of intercellular communication in immune effector, carcinogenesis and metastasis, but also to the development and improvement of diagnosis and therapy of relative diseases. In spite of abundant highly sensitive methods that have been developed for the detection of secreted proteins, majority of them fall short in providing sufficient spatial and temporal resolution for comprehensive profiling of protein secretion from single cells. The real-time imaging techniques allow rapid acquisition and manipulation of analyte information on a 2D plane, providing high spatiotemporal resolution. Here, we summarize recent advances in real-time imaging of secretory proteins from single cell, including label-free and labelling techniques, shedding light on the development of simple yet powerful methodology for real-time imaging of single-cell protein secretion.

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