scholarly journals High-Speed, High-Performance Real-Time Imaging of Physiological Sarcomere Dynamics in the Beating Mouse Heart in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 463a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Takashi Ohki ◽  
Takako Terui ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

scholarly journals Real-Time Imaging of Sarcomere Dynamics in the Mouse Heart In Vivo

2014 ◽  
Vol 106 (2) ◽  
pp. 645a-646a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Akari Mizuno ◽  
Takako Terui ◽  
Togo Shimozawa ◽  
...  
Keyword(s):  
Real Time ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

scholarly journals Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function

2015 ◽  
Vol 147 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Akari Mizuno ◽  
Takashi Ohki ◽  
...  
Keyword(s):  
High Speed ◽  
Sarcomere Length ◽  
Imaging System ◽  
Critical Role ◽  
Left Ventricular Pressure ◽  
Left Ventricular ◽  
Mouse Heart ◽  
Tight Coupling ◽  
Sarcomere Dynamics

Sarcomeric contraction in cardiomyocytes serves as the basis for the heart’s pump functions in mammals. Although it plays a critical role in the circulatory system, myocardial sarcomere length (SL) change has not been directly measured in vivo under physiological conditions because of technical difficulties. In this study, we developed a high speed (100–frames per second), high resolution (20-nm) imaging system for myocardial sarcomeres in living mice. Using this system, we conducted three-dimensional analysis of sarcomere dynamics in left ventricular myocytes during the cardiac cycle, simultaneously with electrocardiogram and left ventricular pressure measurements. We found that (a) the working range of SL was on the shorter end of the resting distribution, and (b) the left ventricular–developed pressure was positively correlated with the SL change between diastole and systole. The present findings provide the first direct evidence for the tight coupling of sarcomere dynamics and ventricular pump functions in the physiology of the heart.

scholarly journals Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

2009 ◽  
Vol 3 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Paul Timpson ◽  
Alan Serrels ◽  
Marta Canel ◽  
Margaret C. Frame ◽  
Valerie G. Brunton ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Real Time ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Real Time Imaging

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

2013 ◽  
Vol 4 (3) ◽  
pp. 1079 ◽  
Author(s):  
Kehua Xu ◽  
Mingming Qiang ◽  
Wen Gao ◽  
Ruixian Su ◽  
Na Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Redox Status ◽  
Real Time Imaging

Development of a high-speed VCSEL OCT system for real-time imaging of conscious patients larynx using a hand-held probe (Conference Presentation)

2016 ◽  
Author(s):  
Swathi Rangarajan ◽  
Li-Dek Chou ◽  
Carolyn Coughlan ◽  
Giriraj Sharma ◽  
Brian J. F. Wong ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Real Time Imaging

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

scholarly journals Monitoring and characterization of vibration and shock conditions aboard high-performance marine craft

2018 ◽  
Vol 233 (4) ◽  
pp. 1068-1081
Author(s):  
Manudul Pahansen de Alwis ◽  
Karl Garme
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Severity Index ◽  
Irregular Waves ◽  
Vibration Exposure ◽  
Data Set ◽  
Real Time Analysis ◽  
Impact Acceleration ◽  
The Impact

The stochastic environmental conditions together with craft design and operational characteristics make it difficult to predict the vibration environments aboard high-performance marine craft, particularly the risk of impact acceleration events and the shock component of the exposure often being associated with structural failure and human injuries. The different timescales and the magnitudes involved complicate the real-time analysis of vibration and shock conditions aboard these craft. The article introduces a new measure, severity index, indicating the risk of severe impact acceleration, and proposes a method for real-time feedback on the severity of impact exposure together with accumulated vibration exposure. The method analyzes the immediate 60 s of vibration exposure history and computes the severity of impact exposure as for the present state based on severity index. The severity index probes the characteristic of the present acceleration stochastic process, that is, the risk of an upcoming heavy impact, and serves as an alert to the crew. The accumulated vibration exposure, important for mapping and logging the crew exposure, is determined by the ISO 2631:1997 vibration dose value. The severity due to the impact and accumulated vibration exposure is communicated to the crew every second as a color-coded indicator: green, yellow and red, representing low, medium and high, based on defined impact and dose limits. The severity index and feedback method are developed and validated by a data set of 27 three-hour simulations of a planning craft in irregular waves and verified for its feasibility in real-world applications by full-scale acceleration data recorded aboard high-speed planing craft in operation.

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