Effect of Crosslinkers on Physical Properties of Gelatin Hollow Tubes for Tissue Engineering Application

Author(s):  
Chu-Hua Chiu ◽  
Han-Chin Shih ◽  
Shyh-Chuan Jwo ◽  
Ming-Fa Hsieh
2016 ◽  
Vol 872 ◽  
pp. 261-265 ◽  
Author(s):  
Wassanai Wattanutchariya ◽  
Atitaya Oonjai ◽  
Kittiya Thunsiri

This study reports the effects of the mixing ratio of hydroxyapatite (HA), silk fibroin (SF) and chitosan (CS) on the physical properties of the scaffold used in tissue engineering. Experimental design based on mixture design was implemented to investigate the degradation rate of the scaffolds fabricated from various ratios of those biomaterials. Furthermore, pore morphology and pore size were evaluated to confirm the compatibility of the scaffold topography for cell growth and adhesion. The results from the study showed that all ratios, except pure HA solution, can be fabricated into porous scaffolds with an interconnected pore structure and appropriate pore sizes to allow all types of human cells to pass through. Furthermore, the scaffold solutions with high CS ratio resulted in a uniform pore structure and lower rates of biodegradation. Therefore, CS is recommended as the main structure because it provides the highest resistance to biodegradation. The scaffolds from various ratios may be applied for different tissue replacements in the near future.


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PLoS ONE ◽  
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Su Ryon Shin ◽  
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