Effects of Quartz and Asbestos on Alveolar Macrophages: Interspecies Comparison and Cell Biological Studies

1990 ◽  
pp. 199-202 ◽  
Author(s):  
A. Seidel ◽  
U. Nieder ◽  
S. Pätzold ◽  
J. Schimmelpfeng ◽  
A. Schmidt ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Interspecies Comparison ◽  
Biological Studies

scholarly journals Cell Size of Alveolar Macrophages: An Interspecies Comparison

1997 ◽  
Vol 105 ◽  
pp. 1261 ◽  
Author(s):  
Fritz Krombach ◽  
Silvia Munzing ◽  
Anne-Marie Allmeling ◽  
J. Tilman Gerlach ◽  
Jurgen Behr ◽  
...  
Keyword(s):  
Cell Size ◽  
Alveolar Macrophages ◽  
Interspecies Comparison

scholarly journals Cell size of alveolar macrophages: an interspecies comparison.

1997 ◽  
Vol 105 (suppl 5) ◽  
pp. 1261-1263 ◽  
Author(s):  
F Krombach ◽  
S Münzing ◽  
A M Allmeling ◽  
J T Gerlach ◽  
J Behr ◽  
...  
Keyword(s):  
Cell Size ◽  
Alveolar Macrophages ◽  
Interspecies Comparison

INTERSPECIES COMPARISON OF iNOS EXPRESSION AND NO FORMATION BY ALVEOLAR MACROPHAGES

Shock ◽  
1997 ◽  
Vol 7 (Supplement) ◽  
pp. 68
Author(s):  
M. Dörger ◽  
N. K. Jesch ◽  
G. Rieder ◽  
J. Behr ◽  
E. Schütze ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Inos Expression ◽  
Interspecies Comparison ◽  
No Formation

Interspecies Comparison of Phagolysosomal pH in Alveolar Macrophages

1991 ◽  
Vol 3 (1) ◽  
pp. 91-100 ◽  
Author(s):  
W. G. Kreyling ◽  
K. Nyberg ◽  
D. Nolibé ◽  
C. G. Collier ◽  
P. Camner ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Interspecies Comparison

Modification of the Electron Microscope for Investigation of Fully Hydrated Biological Specimens

1969 ◽  
Vol 27 ◽  
pp. 418-419 ◽  
Author(s):  
R. C. Moretz ◽  
D. F. Parsons
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Structural Damage ◽  
Lower Percentage ◽  
Vacuum Drying ◽  
Total Removal ◽  
Total Absence ◽  
Biological Studies ◽  
Electron Microscopes ◽  
Beam Damage

Short lifetime or total absence of electron diffraction of ordered biological specimens is an indication that the specimen undergoes extensive molecular structural damage in the electron microscope. The specimen damage is due to the interaction of the electron beam (40-100 kV) with the specimen and the total removal of water from the structure by vacuum drying. The lower percentage of inelastic scattering at 1 MeV makes it possible to minimize the beam damage to the specimen. The elimination of vacuum drying by modification of the electron microscope is expected to allow more meaningful investigations of biological specimens at 100 kV until 1 MeV electron microscopes become more readily available. One modification, two-film microchambers, has been explored for both biological and non-biological studies.

Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

1982 ◽  
Vol 40 ◽  
pp. 332-333
Author(s):  
V. V. Damiano ◽  
R. P. Daniele ◽  
H. T. Tucker ◽  
J. H. Dauber
Keyword(s):  
Quantitative Analysis ◽  
Alveolar Macrophages ◽  
Bulk Sample ◽  
Silica Particles ◽  
Empirical Method ◽  
Phagocytic Cells ◽  
Calibration Standards ◽  
X Ray ◽  
Accurate Quantitative Analysis ◽  
Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

Sign in / Sign up

Export Citation Format

Share Document