C-Banding in Zea mays

Author(s):  
E. K. Gubar ◽  
V. A. Kunakh
Keyword(s):  
Zea Mays ◽  
1983 ◽  
Vol 25 (3) ◽  
pp. 203-209 ◽  
Author(s):  
Ineke Mastenbroek ◽  
J. M. J. de. Wet

An established C-banding technique was modified to consistently yield complete sets of distinct, sharply banded chromosomes in Zea. It was used to demonstrate similarities and differences among heterochromatin patterns of different Zea taxa. The C-banding patterns showed a general agreement with heterochromatic knob positions known from pachytene studies. Two groups of banding patterns could be distinguished. Those taxa with bands that were almost exclusively terminally positioned included Z. mays ssp. parviglumis var. huehuetenangensis, Z. diploperennis, Z. perennis, and Z. luxurians. In the last mentioned, previously unknown heterochromatic regions were found. Both terminal and subterminal band positions were found in Z. mays, Z. mays ssp. mexicana, and Z. mays ssp. parviglumis var. parviglumis. On the basis of these results, and those of other workers, the taxonomic treatments of Z. mays ssp. parviglumis var. huehuetenangensis, and of Z. diploperennis and its autotetraploid derivative Z. perennis are questioned.


Author(s):  
O. E. Bradfute ◽  
R. E. Whitmoyer ◽  
L. R. Nault

A pathogen transmitted by the eriophyid mite, Aceria tulipae, infects a number of Gramineae producing symptoms similar to wheat spot mosaic virus (1). An electron microscope study of leaf ultrastructure from systemically infected Zea mays, Hordeum vulgare, and Triticum aestivum showed the presence of ovoid, double membrane bodies (0.1 - 0.2 microns) in the cytoplasm of parenchyma, phloem and epidermis cells (Fig. 1 ).


Author(s):  
O. E. Bradfute

Maize mosaic virus (MMV) causes a severe disease of Zea mays in many tropical and subtropical regions of the world, including the southern U.S. (1-3). Fig. 1 shows internal cross striations of helical nucleoprotein and bounding membrane with surface projections typical of many plant rhabdovirus particles including MMV (3). Immunoelectron microscopy (IEM) was investigated as a method for identifying MMV. Antiserum to MMV was supplied by Ramon Lastra (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela).


Author(s):  
O. E. Bradfute

Maize rayado fino virus (MRFV) causes a severe disease of corn (Zea mays) in many locations throughout the neotropics and as far north as southern U.S. MRFV particles detected by direct electron microscopy of negatively stained sap from infected leaves are not necessarily distinguishable from many other small isometric viruses infecting plants (Fig. 1).Immunosorbent trapping of virus particles on antibody-coated grids and the antibody coating or decoration of trapped virus particles, was used to confirm the identification of MRFV. Antiserum to MRFV was supplied by R. Gamez (Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica).Virus particles, appearing as a continuous lawn, were trapped on grids coated with MRFV antiserum (Fig. 2-4). In contrast, virus particles were infrequently found on grids not exposed to antiserum or grids coated with normal rabbit serum (similar to Fig. 1). In Fig. 3, the appearance of the virus particles (isometric morphology, 30 nm diameter, stain penetration of some particles, and morphological subunits in other particles) is characteristic of negatively stained MRFV particles. Decoration or coating of these particles with MRFV antiserum confirms their identification as MRFV (Fig. 4).


1995 ◽  
Vol 95 (3) ◽  
pp. 409-414 ◽  
Author(s):  
Pierre Haldimann ◽  
Yvan Fracheboud ◽  
Peter Stamp

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