Transmission characteristics of allexiviruses by the eriophyid mite, Aceria tulipae (Keifer) (Acari: Eriophyidae) from naturally mixed infected garlic (Allium sativum L.)

The transmission characteristics of members of the genus Allexivirus to leek (Allium porrum L.) by its eriophyid mite vector, Aceria tulipae (Keifer), were studied. Prior to conducting transmission tests, colonies of nonviruliferous A. tulipae were established on healthy leek seedlings. A single A. tulipae transmitted the viruses with up to 50 % efficiency but transmission efficiency increased when > 10 mites per plant were used. Allexiviruses were acquired by A. tulipae after a minimum acquisition access period (AAP) of 30 minutes, whereas transmission tests suggest that a one hour inoculation access period (IAP) was needed for successful transmission. Allexiviruses were transmitted from garlic to leek plants by A. tulipae and mixed infections by more than one virus were observed. ShVX, GarV-A, -C, -D, and -B were detected in most inoculated plants, whereas other members of the genus (GarV-E, -X, and GarMbFV) were found only occasionally. None of the mites that originated from eggs deposited on infected plants transmitted allexiviruses, indicating that the viruses are not transmitted transovarially. No latent period was demonstrated. Taken together, these data suggest a semipersistent mode of transmission of Allexivirus members by A. tulipae. The output of this study will assist in the better management of the vector and the associated diseases.

Plodia interpunctella (Hübner, 1813) and Nemapogon granellus (Linnaeus, 1758) – Uncommon Pests on Stored Garlic (Allium sativum L.) in the Czech Republic

Garlic is one of several important vegetable species grown in the Czech Republic. Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae), Aceria tulipae (Keifer, 1938) (Acari: Eriophyidae), Rhizoglyphus echinopus (Fumouze & Robin, 1868) (Acari: Acaridae) and R. robinii Claparède, 1869 (Acari: Acaridae), Suillia lurida (Meigen, 1830) (Diptera: Heleomyzidae) are common pest species found on garlic plants in the Czech Republic. Nowadays Aceria tulipae (Keifer, 1938) is beginning to be considered as the one causing damage. Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae) and Nemapogon granellus (Linnaeus, 1758) (Lepidoptera: Tineidae) are small moths which are common pests usually found on stored products. Both of these species larvae were found as the uncommon pests of stored garlic in the Czech Republic in January 2016. Until now, their harmfulness on stored garlic has been reported only from Central America.

Infestation of different garlic varieties by dry bulb mite Aceria tulipae (Keifer) (Acari: Eriophyidae)

The intensity of garlic infestation by dry bulb mite Aceria tulipae (Keifer, 1938) was observed on different garlic varieties in central Moravia. The aim of this study was to determine infestation of different garlic varieties during storage in the winter period 2011–2012. Current studies on 11 garlic varieties from the Centre of the Region Haná at an altitude of 210 m showed high abundance of Aceria tulipae on 10 varieties. Bolting garlic varieties (Bzenecky Mutant VF, Sochi 25, Tiraspol, Zailijskij) were highly infested, non-bolting varieties (Czerga, SIR 10 new breeding, Gjirokaster) were infested weakly or not at all (Kelcyre). The highest abundance of mite was found out in semi-bolting garlic variety (Plovdiv Rogosh) with total number up to 1 500 individuals in one clove. Significant differences in infestation between external and internal part of the clove were observed in 4 of 11 evaluated varieties. Root segment was significantly the most infested part of the clove. The most resistant kinds to mite infestation were the Kelcyre, Gjirokaster and SIR 10 new breeding varieties. The highest mite introduction to inside cloves was observed in the Plovdiv Rogosh variety. The choice of suitable varieties can significantly eliminate occurrence of A. tulipae and their infestation.

Transmission of a chromosome from decaploid Agropyron elongatum that confers resistance to the wheat curl mite in common wheat

One of the group 6 chromosomes from Agropyron elongatum (Host) Beauv. confers resistance to colonization by the wheat curl mite (Aceria tulipae Keifer). The transmission of this chromosome was evaluated when added to or substituted for the group 6 homoeologues in two cultivars of common wheat (Triticum aestivum L.). Female transmission of the alien chromosome was similar for all substitutions (8.9%) but significantly less than expected. Male transmission (39.7%) differed among the substituted chromosomes and also was lower than expected except for the substitution involving chromosome 6A. These reduced transmission frequencies were reflected in lower than expected resistance in the F2 progeny. Both male (14.5%) and female (5.2%) transmission of the alien chromosome were reduced in monosomic additions. Meiotic analyses detected all expected F2 cytotypes but some of their frequencies differed among the substituted chromosomes. A possible wheat –alien translocation was detected in BC1F1 progeny.Key words: Agropyron elongatum, Thinopyrum, Elytrigia, Lyphopyrum, wheat curl mite, chromosome substitution.