An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques

1998 ◽  
pp. 99-128 ◽  
Author(s):  
R. J. Snowdon ◽  
A. Langsdorf
Keyword(s):  
Dna Fingerprinting

DNA fingerprinting of medicinally used Derris species by RAPD molecular markers

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
S Sukrong ◽  
T Phadungcharoen ◽  
N Ruangrungsi
Keyword(s):  
Molecular Markers ◽  
Dna Fingerprinting

Construction of DNA fingerprinting database of germplasm materials of Porphyra haitanensis

2010 ◽  
Vol 34 (6) ◽  
pp. 733-740 ◽  
Author(s):  
Chao-tian XIE ◽  
Chang-sheng CHEN ◽  
De-hua JI ◽  
Guo-rui ZHAO ◽  
Yan XU ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Porphyra Haitanensis

Identification of Western Wheatgrass Cultivars and Accessions by DNA Fingerprinting and Geographic Provenance

Crop Science ◽  
2003 ◽  
Vol 43 (1) ◽  
pp. 394 ◽  
Author(s):  
S. R. Larson ◽  
A. J. Palazzo ◽  
K. B. Jensen
Keyword(s):  
Dna Fingerprinting ◽  
Western Wheatgrass

LEGITIMACY OF PROGENIES USING SSR MARKERS AS CONTROLLING SYSTEM AND EARLIER SELECTION IN THE OIL PALM NURSERY (Elaeis guineensis JACQ.)

2018 ◽  
Vol 25 (1) ◽  
pp. 21-30
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Abdul Razak Purba
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oil Palm ◽  
Dna Markers ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Controlling System ◽  
Crossing Probability ◽  
Simple Sequence

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

OPTIMATION OF DNA EXTRACTION PROTOCOL OF Elaeidobius kamerunicus

2018 ◽  
Vol 24 (2) ◽  
pp. 77-86
Author(s):  
Sri Wening ◽  
Agus Eko Prasetyo ◽  
Tjut Ahmad Perdana Rozziansha ◽  
Agus Susanto
Keyword(s):  
Dna Fingerprinting ◽  
Dna Extraction ◽  
Oil Palm ◽  
Fruit Set ◽  
Fragment Length Polymorphism ◽  
Basic Knowledge ◽  
Biological Study ◽  
Palm Fruit ◽  
Extraction Protocol ◽  
Elaeidobius Kamerunicus

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.

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